Assessment of Bacillus pumilus Isolated from Fresh Water Milieu for Bioflocculant Production

Makapela, Busisiwe; Okaiyeto, Kunle; Ntozonke, Ncedo; Nwodo, Uchechukwu U.; Green, Ezekiel; Mabinya, Leonard V.; Okoh, Anthony I.

doi:10.3390/app6080211

Cited by 33 publications

(30 citation statements)

References 65 publications

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“…Similar findings were reported by Makapela et al (2016) with a strain of B. pumilus from Thyume River in Alice, Eastern Cape (South Africa). For this strain, maltose and sucrose were the favorable carbon sources for the production of bioflocculants (respectively 71.7% and 69.8% of activities), after 120 h of incubation in a production medium composed of (g.L -1 ): maltose, 20; K 2 HPO 4 , 5; KH 2 PO 4 , 2, urea 0.5; yeast extract, 0.5; (NH 4 ) 2 SO 4 , 0.2 and MgSO 4 .7H 2 O, Figure 2.…”

Section: Optimization Of Culture Medium For Bioflocculant Productionsupporting

confidence: 88%

OPTIMIZATION OF BIOFLOCCULANT PRODUCTION BY Bacillus spp. FROM SUGARCANE CROP SOIL OR FROM SLUDGE OF THE AGROINDUSTRIAL EFFLUENT

Gouveia

Silva

Santos

et al. 2019

Braz. J. Chem. Eng.

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Biological flocculants are advantageous due to their biodegradability and safety to living beings. In this work, four bacterial strains, isolated from sugarcane cropped soil (Bacillus megaterium LBPMA-APFSG3Isox and B. toyonensis LBPMA-ACOPR1.Isox) and sludge of an agroindustrial effluent (B. pumilus LBPMA-BLD07 and B. thuringiensis LBPMA-EFIII), were studied. It was found that all of them secreted bioflocculants, at 37 ± 1 °C, with no pH changes over time and the flocculant activity increased during the time course of incubation. These results stimulated the optimization of the culture conditions to improve flocculation rates, such as the pH, nitrogen sources and carbon. For B. toyonensis LBPMA-ACOPR1.Isox and B. thuringiensis LBPMA-EFIII, the best pH for the bioflocculant production was 5.0. Sucrose and maltose were the best sources of carbon, while urea was the preferred source of nitrogen for two of the tested isolates (B. pumilus LBPMA-BLD07 and B. toyonensis LBPMA-ACOPR1.Isox), followed by (NH 4)2SO 4 (B. megaterium LBPMA-APFSG3Isox) and peptone (B. thuringiensis LBPMA-EFIII). The FTIR-ATR spectra of each extracted material responsible for the flocculant activities of the strains displayed carboxyl, hydroxyl and methoxy functional groups characteristic of polysaccharides.

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Section: Optimization Of Culture Medium For Bioflocculant Productionsupporting

confidence: 88%

OPTIMIZATION OF BIOFLOCCULANT PRODUCTION BY Bacillus spp. FROM SUGARCANE CROP SOIL OR FROM SLUDGE OF THE AGROINDUSTRIAL EFFLUENT

Gouveia

Silva

Santos

et al. 2019

Braz. J. Chem. Eng.

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“…The pH of the cultivation environment significantly influences the electric charge of the microbial cell, consequently affecting the absorption of nutrients and intracellular enzymatic activities [33,34]. The effect of initial medium pH on exoperoxidase production by Ensifer adhaerens NWODO-2 is presented in Figure 1a.…”

Section: Determination Of Process Parameters For Optimal Exoperoxidasmentioning

confidence: 99%

Exoproduction and Molecular Characterization of Peroxidase from Ensifer adhaerens

et al. 2019

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The increased industrial application potentials of peroxidase have led to high market demand, which has outweighed the commercially available peroxidases. Hence, the need for alternative and efficient peroxidase-producers is imperative. This study reported the process parameters for enhanced exoperoxidase production by Ensifer adhaerens NWODO-2 (accession number: KX640918) for the first time, and characterized the enzyme using molecular methods. Peroxidase production by the bacteria was optimal at 48 h, with specific productivity of 12.76 U mg−1 at pH 7, 30 °C and 100 rpm in an alkali lignin fermentation medium supplemented with guaiacol as the most effective inducer and ammonium sulphate as the best inorganic nitrogen source. Upon assessment of some agricultural residues as sources of carbon for the enzyme production, sawdust gave the highest peroxidase productivity (37.50 U mg−1) under solid-state fermentation. A search of the polymerase chain reaction (PCR)-amplified peroxidase gene in UniProtKB using blastx showed 70.5% similarity to an uncharacterized protein in Ensifer adhaerens but phylogenetic analysis suggests that the gene may encode a catalase-peroxidase with an estimated molecular weight of approximately 31 kDa and isoelectric point of about 11. The nucleotide sequence of the detected gene was deposited in the GenBank under the accession number MF374336. In conclusion, the ability of the strain to utilize lignocellulosic materials for peroxidase production augurs well for biotechnological application as this would greatly reduce cost, which is a major challenge in industrial enzyme production.

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“…The bio-flocculant fermentation medium was composed using the following: 10 g of glucose, 1 g of peptone, 0.3 g of MgSO4.7H2O, 5 g of K2HPO4 in one litre of distilled/sterile water. The initial pH was adjusted to 7.0 with either NaOH (0.1M) or HCl (0.1M) (Busisiwe et al, 2016).…”

Section: Media Preparation and Medium Constitutionmentioning

confidence: 99%

Screening for Bio-flocculant Producing Bacterial Strains from Asa River in Ilorin Kwara State

Kolawole

Yahaya

Lawal

et al. 2019

Annals of Science and Technology

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Synthetic flocculants in water treatment have been reported to be detrimental to both human health and the environment. Thus, there is a constant search for bio-flocculants that is safe and addresses the effects of synthetic polymers. This study was aimed at isolating bacteria with bio-flocculating potential, their molecular identification and phylogenetic relatedness, and the optimization of their flocculating abilities from an open water (Asa River) in Ilorin Kwara State. The effect of carbon source (glucose, lactose and starch), cations (FeSO4, KCl and CaCl2) and pH (2 to 12) was evaluated on the bio-flocculating activities of the isolates using kaolin clay. The seven (7) novel (new strains) isolates with their respective accession number from National Center for Biotechnology Information (NCBI) after molecular confirmation are Pseudomonas otitidis MTK01 (MK263227), Aeromonas caviea MTK02 (MK263228), Providencia alcalifaciens MTK03 (MK263229), Providencia sp. MTK05 (MK263230), Alcaligenes sp. MTK06 (MK263231), Klebsiella pneumoniae MTK07 (MK263232) and Klebsiella sp. MTK08 (MK263233) while Raoultella ornithinolytica MTK04 was also identified. Phylogenetic tree of relatedness showed close kin of the isolates to established bacteria sequence deposited at the NCBI GenBank. Although the flocculating rate of each isolates varied with different parameters that was used in the study, glucose was the most supportive followed by lactose and starch, CaCl2 was most supportive cation followed by KCl and FeSO4 while pH 12, 6, 8, 10, 2 and 4 were the order of decreasing flocculating rate of the medium. This study has reported the presence of eight (8) bio-flocculating bacteria (out of which 7 are new strains of bacteria) in an open water which has been further optimized for effective flocculating rate and thus provides an ecofriendly and harmless flocculants source that can be employed in water treatment procedures.

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Assessment of Bacillus pumilus Isolated from Fresh Water Milieu for Bioflocculant Production

Cited by 33 publications

References 65 publications

OPTIMIZATION OF BIOFLOCCULANT PRODUCTION BY Bacillus spp. FROM SUGARCANE CROP SOIL OR FROM SLUDGE OF THE AGROINDUSTRIAL EFFLUENT

OPTIMIZATION OF BIOFLOCCULANT PRODUCTION BY Bacillus spp. FROM SUGARCANE CROP SOIL OR FROM SLUDGE OF THE AGROINDUSTRIAL EFFLUENT

Exoproduction and Molecular Characterization of Peroxidase from Ensifer adhaerens

Screening for Bio-flocculant Producing Bacterial Strains from Asa River in Ilorin Kwara State

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