Assessment of Bacterial Sealing Ability of Two Different Bio-Ceramic Sealers in Single-Rooted Teeth Using Single Cone Obturation Technique: An In Vitro Study

AlEraky, Doaa M.; Rahoma, Ahmed; Abuohashish, Hatem M.; AlQasser, Abdullh; AlHamali, Abbas; AlHussain, Hussain M.; AlShoalah, Hussain M.; AlSaghah, Zakrya; Khattar, Abdulrahman; Rifaat, Shimaa

doi:10.3390/app13052906

Cited by 5 publications

(4 citation statements)

References 42 publications

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“…This finding aligns with our descriptive CLSM images analysis. Furthermore, the antibacterial properties of calcium silicate sealers may contribute to the elimination of any remaining microorganisms [ 43 ], thereby potentially enhancing the success rate of root end treatment.…”

Section: Discussionmentioning

confidence: 99%

Bacterial sealing ability of calcium silicate-based sealer for endodontic surgery: an in-vitro study

Mansour,

Moussa,

Meheissen

et al. 2024

BMC Oral Health

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Background Apical surgery with standard retrograde maneuvers may be challenging in certain cases. Simplifying apical surgery to reduce operating time and streamline retrograde manipulation is an emerging need in clinical endodontics. Aim of the study The aim of the study was to compare the bacterial sealing ability of a calcium silicate-based sealer with the single cone technique combined with root end resection only, and calcium silicate-based sealer as a retrograde filling versus MTA retrofilling, and to analyze bacterial viability using confocal laser scanning microscope (CLSM). Materials and methods In this in vitro experimental study, 50 extracted human maxillary incisor teeth were instrumented and randomly divided into five groups: three experimental groups, a positive control group, and a negative control group (n = 10/group). In the experimental groups, the roots were obturated using the single cone technique (SCT) and a calcium silicate-based sealer. In group 1, the roots were resected 3 mm from the apex with no further retrograde preparation or filling. In groups 2 and 3, the roots were resected, retroprepared, and retrofilled with either a calcium silicate-based sealer or MTA, respectively. Group 4 (positive control) was filled with a single gutta-percha cone without any sealer. In group 5 (negative control), the canals were left empty, and the roots were sealed with wax and nail varnish. A bacterial leakage model using Enterococcus faecalis was employed to assess the sealing ability over a 30-day period, checking for turbidity and analyzing colony forming units (CFUs) per milliliter. Five specimens from each group were examined using CLSM for bacterial viability. Data for the bacterial sealing ability were statistically analyzed using chi-squared and Kruskal-Wallis tests. Results The three experimental groups did not show significant differences in terms of bacterial leakage, or bacterial counts (CFUs) (P > 0.05). However, significant differences were observed when comparing the experimental groups to the positive control group. Notably, the calcium silicate-based sealer, when used as a retrofilling, yielded the best sealing ability. CLSM imaging revealed viable bacterial penetration in all the positive control group specimens while for the experimental groups, dead bacteria was the prominent feature seen. Conclusion Within the limitations of this study, it could be concluded that the bacterial sealing ability of calcium silicate-based sealer with the single cone technique combined with root end resection only and calcium silicate-based sealer as a retrograde filling were comparable with MTA retrofilling during endodontic surgical procedures.

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Section: Discussionmentioning

confidence: 99%

Bacterial sealing ability of calcium silicate-based sealer for endodontic surgery: an in-vitro study

Mansour,

Moussa,

Meheissen

et al. 2024

BMC Oral Health

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“…In this study, AH Plus (Dentsply) sealer was chosen for its renowned ease of handling, sealability, and favorable biological performance in endodontic procedures [ 8 ]. The micro-retention of radicular dentin by AH Plus (Dentsply) contributes to enhanced adhesion to obturating materials compared to other sealers [ 12 , 13 ].…”

Section: Discussionmentioning

confidence: 99%

“…Chitosan is a polysaccharide that naturally occurs, and because it is non-toxic, biocompatible, ecological, and bioadhesive in nature, it has attracted interest in dentistry research [5][6][7] Among the many sealants used for root canal treatment, AH Plus (Dentsply Sirona, NC, USA) sealant has gained popularity due to its ability to improve dentin wettability, and gutta-percha sealability with adequate biological performance [8,9].…”

Section: Introductionmentioning

confidence: 99%

Effect of Chitosan Irrigant and Lubricating Gel on Bond Strength of Resin Sealer to Radicular Dentin: An In Vitro Study

Kumar,

Pai,

Joshi

et al. 2024

Cureus

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Background: The adhesive strength of sealers to dentin is influenced by various factors, and the presence of a smear layer is among the critical variables. Chitosan, known for its dentin compatibility, has previously demonstrated a reduction in dentin change and resin sealer bond strength comparable to ethylenediaminetetraacetic acid (EDTA) when used as an irrigant and final rinse. The study investigates the impact of chitosan, used as both a lubricating gel and final rinse, on the push-out bond strength of resin sealer.Materials and method: Forty single-rooted premolar teeth, each with a fully formed root and a single root canal, were collected post-extraction. During canal preparation, 1 ml sodium hypochlorite (3%) was used for irrigation at every change of instrument, followed by applying specific chelating gel and final rinse for each experimental group. The groups included: Group 1 (17% EDTA chelating gel, final rinse with saline), Group 2 (17% EDTA chelating gel, final rinse with 17% EDTA solution), Group 3 (chitosan chelating gel, final rinse with saline solution), and Group 4 (chitosan chelating gel, final rinse with 0.2% chitosan solution), 10 specimens in each group. After obturation, specimens were sealed and incubated for a week at 37°C with 100% humidity. The universal testing machine was used for push-out tests, and specimens were examined using a scanning electron microscope (SEM) to identify various types of bond failure.Results: Among the four groups, Group 2 exhibited the highest mean push-out bond strength (7.33 ± 0.26 MPa), followed by Group 4 (5.33 ± 0.25 MPa), Group 1 (4.61 ± 0.30 MPa), and Group 3 (2.94 ± 0.32 MPa). The variations in bond strength suggest a notable impact of the chelating agents and final rinse solutions on the resin sealer's interaction with dentin. Conclusion:The study concludes that the use of EDTA as both a lubricating gel and a final rinse significantly enhances push-out bond strength, outperforming chitosan in this study. Groups with saline as the final rinse (Group 1 and Group 3) exhibited the least bond strength, highlighting the importance of the final rinse in root canal therapy.

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“…The MTA-BCS have received scientific interest due to their superior properties in comparison to other sealers. Previous studies showed variations in properties of different commercially available MTA-BCS which might affect quality and durability (9)(10)(11) . The aim of the current study was to evaluate 3 commercially available MTA-BCS sealers regarding flow, film thickness and setting time.…”

Section: Introductionmentioning

confidence: 99%

Flow, Film thickness and Setting Time Properties of Three Mineral Trioxide Aggregate Based Bio-ceramic Sealers: An in Vitro Study

2023

The Egyptian Journal of Hospital Medicine

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Background: The mineral trioxide aggregate (MTA) based bio-ceramic (MTA-BCS) has become quite well known for its top-notch amenities. The quality of the obturation procedure is influenced by the composition and handling characteristics of the root canal sealers. Flow, film thickness and setting time are the most important handling properties as they affected the ability of the sealers to enter narrow and irregular spaces. Objective: The aim of the current study was to evaluate 3 commercially available MTA-BCS sealers regarding flow, film thickness and setting time. Materials and methods: Three commercially available MTA based sealers were used in this study. Samples were divided into three groups; Group I (MTA fillapex), Group II (Ceramoseal HBC) and Group III (Endoseal MTA). Flow, film thickness, initial and final setting time were tested according to ISO 6876 standards. Results: For the flow, test Group I showed the highest mean value (26.50±0.50 mm) and the lowest statistically significant mean value was Group II (22.59±1.94 mm). For the film thickness, the highest statistically significant mean value was recorded in Group II (29.4±4.72 μm) while the lowest mean value was recorded in Group III (15±3.53μm). For initial setting time, the highest statistically significant mean value was recorded in Group I (858.51±11.82 min). The lowest statistically significant mean value was recorded in Group III (35.2±2.86 min). For final setting time, group I was unable to set up to one month. Group III was the shortest statistically significant mean value (150.4±3.44 min) compared to Group II (832±6.78 min). Conclusion:Within the limitation of this study, it can be concluded that all the tested sealers fulfilled the requirements of ISO specifications no 6876 for flow and film thickness. Regarding setting time, only Group II fulfilled their manufacturer declaration for initial setting as stated by ISO.

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Assessment of Bacterial Sealing Ability of Two Different Bio-Ceramic Sealers in Single-Rooted Teeth Using Single Cone Obturation Technique: An In Vitro Study

Cited by 5 publications

References 42 publications

Bacterial sealing ability of calcium silicate-based sealer for endodontic surgery: an in-vitro study

Bacterial sealing ability of calcium silicate-based sealer for endodontic surgery: an in-vitro study

Effect of Chitosan Irrigant and Lubricating Gel on Bond Strength of Resin Sealer to Radicular Dentin: An In Vitro Study

Flow, Film thickness and Setting Time Properties of Three Mineral Trioxide Aggregate Based Bio-ceramic Sealers: An in Vitro Study

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