Assessment of Bandsharing Values in RAPD-PCR Analysis of Dwarf Cattle of Kerala

Abstract

Random amplified polymorphic DNA (RAPD-PCR) analysis of 56 animals of four different genetic groups of dwarf cattle in Kerala was done as a single step analysis. Bandsharing (BS) values were calculated for animals of each group and between groups as an analytical tool to find out genetic variation among animals. The different factors affecting BS values were estimated using Harvey's Least squares analysis. The effects of genetic group, Guanine-cytosine (GC) content of primer and gel on BS values were found significant. Bandsharing values of Kasargode-Highrange dwarf animals were significantly different from Vechur, Vatakara and their combinations. The Vechur, Vatakara and Vechur-Vatakara combinations were found to be more uniform (high BS value) compared with other combinations. The bandsharing value was lowest with primers of GC content 90% and highest with 80% GC content. The effect of gel on BS value points to the need of adjustments of gel factor for calculation of BS values.

Assessment of Genetic Variability in Two North Indian Buffalo Breeds Using Random Amplified Polymorphic DNA (RAPD) Markers

“…Genetic characterization of native breeds is the first step in prioritization of breeds for conservation. In the recent past, RAPD-PCR approach has been used successfully in numerous studies to reveal informative polymorphism in several species and characterization of genetic variability among breeds of species (Caetano-Anolles et al, 1991;Hunt et al, 1992;Kantanen et al, 1995;Mohd-Azmi et al, 2000;Shilin et al, 2001;Appannavar et al, 2002;Saifi et al, 2004;Suprabha et al, 2005). Technically RAPD assay is simple, rapid, sensitive and low cost method for producing much polymorphic information of the target genome.…”

Analysis of Geographical Genetic Differences of Arkshell Populations in Korea

“…https://doi.org/10.12717/DR.2021.25.2.105 population identification in finfish and crustaceans (Smith et al, 1997;Klinbunga et al, 2000;McCormack et al, 2000;Yoon & Park, 2002;Park et al, 2005;Siti Azizah et al, 2005), plant (Bartish et al, 2000;Nozaki et al, 2000) and cattle (Suprabha et al, 2005). PCR-based analysis can determine the genetic characteristics of individuals and populations, as highlighted by the substantial GD observed between the two Scapharca populations.…”

“…The Euclidean GDs between two populations of bastard halibut ( Paralichthys olivaceus ) were determined to be 0.018–0.489 from the pooled data for the eight primers used ( Yoon, 2018 ). This technique might help define the diagnostic markers related to the breed, species, genus, and geographic population identification in finfish and crustaceans ( Smith et al, 1997 ; Klinbunga et al, 2000 ; McCormack et al., 2000 ; Yoon & Park, 2002 ; Yoon & Kim, 2004 ; Park et al, 2005 ; Siti Azizah et al, 2005 ), plant ( Bartish et al, 2000 ; Nozaki et al, 2000 ) and cattle ( Suprabha et al, 2005 ). PCR-based analysis can determine the genetic characteristics of individuals and populations, as highlighted by the substantial GD observed between the two Scapharca populations.…”