Assessment of Beta-lactamases and Integrons Genes among Bacteria Isolated From Bladder Cancer Patients with Urinary Tract Infections

Abstract

Background and Aim: One of the most dangerous side effects and the main factor in both morbidity and mortality in cancer patients are infections. Urinary tract infections (UTIs) are among the most prevalent infections in cancer patients. The Recent study aimed to isolation , identification of bacteria from bladder cancer patients with UTIs treated with BCG OR Mitomycin C (MMC) and characterized the presence of bla SHV , bla IMP by PCR in addition to detection the prevalence of Integron gene in the isolated bacterium . Methods: Two hundred urine samples were taken from patients with bladder cancer one hundred from bladder cancer patients treated with BCG (Group-1) and one hundred treated with Mitomycin C (Group -II) between the dates of 1 April 2021 and 15 October 2021 while they were enrolled in third floor Ghazi Al-Hariri Hospital, Medical City, Baghdad Province, and private clinics in Nasiriyah Province. Forty urine samples were taken from patients with UTI without Bladder Ca (Control ,Group -III). Bacterial strains were identified using the Indole test ,Oxidase test , β-hemolytic activity, API20 E test and by 16sRNA . Results: The most commonly pathogens were Escherichia coli , followed by Klebsiella pneumoniae , Pseudomonas aeruginosa and Staphyllococcus aureus and other genera of UTI bacterium. The total BlaSHV Producers in the current study from 38 isolates were 18 isolate(47.3%).The highest BlaSHV Producer in all Groups was E.coli followed by K.pneumoniae and P.aeruginosae . None of all tested isolates were positive to BlaIMP gene while the presence of Integron class 1 was detected in( 34 )89.4% of total isolates.