Assessment of Biocompatibility and Vascular Effects of Polymeric Materials Using Chick Chorioallantoic Membrane, an Alternative Method

Abstract

During the development of polymeric wound coating materials, the in vivo reactions play a key role in the success of the material for tissue engineering. In this work, the chick chorioallantoic membrane (CAM) model is highlighted as an alternative test to evaluate the biocompatibility and changes through the vascular network topography of CAM after the incubation with polymeric membranes. The eggshells of fertilized chicken eggs are opened on the third day of embryo development and the CAM is exposed. Polymeric membranes are placed on the CAM surface and incubated for 96 h. After, the CAM is photographed, the vessels are quantified and histological sections are prepared. In the groups that received spunbonded and cellulose, it is possible to observe tissue growth of the CAM involving the membranes. Regarding the changes in the vascular metrics, a significant increase in the total vessel length and the number of junctions is detected for the spunbonded group. Also, there is an expressive decrease of the lacunarity for this group. Histological analysis revealed an increase of new vessels on the spunbonded, which corroborates to the results mentioned before. The results obtained point out that the CAM assay provides a new perspective on the biocompatibility tests.

Cu2O Nanocubes Embedded in Polycaprolactone Nanofibers for Photo–chemotherapeutic Wound Disinfection and Regeneration

“…The biocompatibility of various samples was further evaluated in ovo embracing the chorioallantoic membrane (CAM) assay concept. 33 To do that, fertilized chicken eggs were maintained in a humidified incubator at 37 °C for 9−10 days to allow embryo development. Then, a circular opening (∼2.5 cm 2 ) was implemented in the eggshell air chamber.…”