Assessment of biofilm formation in Pseudomonas aeruginosa by antisense mazE-PNA

Valadbeigi, Hassan; Sadeghifard, Nourkhoda; Salehi, Majid Baseri

doi:10.1016/j.micpath.2017.01.009

Cited by 11 publications

(5 citation statements)

References 8 publications

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“…Upon oxidative stress, the antitoxin MqsA is degraded by Lon and ClpXP proteases, and, as a result, the stringent response is triggered, and the bacterial state changed from planktonic to sessile (biofilm) [ 64 ]. In the case of the widespread mazEF TA system [ 65 , 66 ], it was recently shown that the antitoxin MazE from Pseudomonas aeruginosa participates in biofilm formation, rather than the toxin MazF [ 67 ]. Further, genes responsive to stress are triggered during colonization, and they also participate in biofilm formation which, in turn, is influenced by multiple TA systems (extensively reviewed in References [ 57 , 68 ]).…”

Section: Discussionmentioning

confidence: 99%

The Streptococcus pneumoniae yefM-yoeB and relBE Toxin-Antitoxin Operons Participate in Oxidative Stress and Biofilm Formation

Chan¹,

Domenech

Moreno-Córdoba

et al. 2018

Toxins

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Type II (proteic) toxin-antitoxin systems (TAs) are widely distributed among bacteria and archaea. They are generally organized as operons integrated by two genes, the first encoding the antitoxin that binds to its cognate toxin to generate a harmless protein–protein complex. Under stress conditions, the unstable antitoxin is degraded by host proteases, releasing the toxin to achieve its toxic effect. In the Gram-positive pathogen Streptococcus pneumoniae we have characterized four TAs: pezAT, relBE, yefM-yoeB, and phD-doc, although the latter is missing in strain R6. We have assessed the role of the two yefM-yoeB and relBE systems encoded by S. pneumoniae R6 by construction of isogenic strains lacking one or two of the operons, and by complementation assays. We have analyzed the phenotypes of the wild type and mutants in terms of cell growth, response to environmental stress, and ability to generate biofilms. Compared to the wild-type, the mutants exhibited lower resistance to oxidative stress. Further, strains deleted in yefM-yoeB and the double mutant lacking yefM-yoeB and relBE exhibited a significant reduction in their ability for biofilm formation. Complementation assays showed that defective phenotypes were restored to wild type levels. We conclude that these two loci may play a relevant role in these aspects of the S. pneumoniae lifestyle and contribute to the bacterial colonization of new niches.

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Section: Discussionmentioning

confidence: 99%

The Streptococcus pneumoniae yefM-yoeB and relBE Toxin-Antitoxin Operons Participate in Oxidative Stress and Biofilm Formation

Chan¹,

Domenech

Moreno-Córdoba

et al. 2018

Toxins

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“…These mostly include phages infecting Escherichia species, together with phages infecting Pseudomonas aeruginosa, Caulobacter crescentus and Acinetobacter baumannii respectively, as well as one phage with the broad host range that includes Escherichia and Pseudomonas species [Olsen and Shipley 1973] ( Table 1 ). mazF homologs have been also found in the genomes of P. aeruginosa and A. baumannii , however their sequence specificity has not been investigated so far [Jurenaite et al 2013, Valadbeigi et al 2017]. Here we analyzed the frequency of ACA sites in the collected genomes, and found that the ACA trinucleotide was less frequent than expected in the RNA phage genomes, i.e.…”

Section: Resultsmentioning

confidence: 90%

Bacterial toxin-antitoxin system MazEF as a native defense mechanism against RNA phages inEscherichia coli

Nikolić

Bergmiller

Pleška

et al. 2023

Preprint

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Bacteria have evolved a wide range of defense strategies to protect themselves against bacterial viruses (phages). However, the known mechanisms almost exclusively target phages with DNA genomes. While several bacterial toxin-antitoxin systems have been considered to cleave single-stranded bacterial RNA in response to stressful conditions, their role in protecting bacteria against phages with single-stranded RNA genomes has not been studied. Here we investigate the role of a representative toxin-antitoxin system, MazEF, in protecting Escherichia coli against two RNA phages - MS2 and QBeta. Our population-level experiments revealed that a mazEF deletion strain is more susceptible to RNA phage infection than the wild-type. At the single-cell level, deletion of the mazEF locus significantly shortened the time to lysis of individual bacteria challenged with RNA phage. At the genomic level, we found that the adenine-cytosine-adenine sequence, directly recognized and cleaved by the MazF toxin, is systematically underrepresented in the genomes of RNA phages that are known to infect E. coli, indicating selection for decreased probability of cleavage. These results suggest that in addition to other physiological roles, RNA-degrading toxin-antitoxin modules can function as a primitive immune system against RNA phages.

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“…As we know, there are different types among TA systems, of which type II is the most famous and well-known [ 14 ] so, TA systems such as higBA , mazEF , and mqsRA can be potentially associated with biofilm formation [ 33 ]. Different studies showed a correlation of TA systems and biofilm formation.…”

Section: Discussionmentioning

confidence: 99%

Effect of ZnO nanoparticles on biofilm formation and gene expression of the toxin-antitoxin system in clinical isolates of Pseudomonas aeruginosa

Valadbeigi,

Sadeghifard,

Kaviar

et al. 2023

Ann Clin Microbiol Antimicrob

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Background Biofilm formation by Pseudomonas aeruginosa (P. aeruginosa) is known to be characteristic of this organism. This bacterium is considered one of the most life-threatening bacteria and has been identified as a priority pathogen for research by WHO. Biofilm-producing P. aeruginosa is a concern in many parts of the world due to antibiotic resistance. Alginate also plays an important role in the biofilm formation of P. aeruginosa as well as the emergence of antibiotic resistance in biofilms. In addition, the systems of toxin-antitoxin( TA) play an important role in biofilm formation. Metal nanoparticle(NP) such as zinc oxide (ZnO) also have extensive biological properties, especially anti-biofilm properties. Therefore, this study was conducted in relation to the importance of zinc oxide nanoparticles (ZnO NPs) in biofilm formation and also the correlation of gene expression of TA systems in clinical isolates of P. aeruginosa. Methods A total of 52 P. aeruginosa isolates were collected from burns (n = 15), UTI (n = 31), and trachea (n = 6) in hospitals in Ilam between May 2020 and October 2020. Biofilm formation was assessed using a microtiter plate assay. MIC and sub-MIC concentrations of ZnO NPs (10–30 nm with purity greater than 99.8%) in P. aeruginosa were determined. Subsequently, biofilm formation was investigated using sub-MIC concentrations of ZnO NPs. Finally, total RNA was extracted and RT- qPCR was used to determine the expression levels of genes of mazEF, mqsRA, and higBA of TA systems. Results Six isolates of P. aeruginosa were found to form strong biofilms. The results showed that ZnO NPs were able to inhibit biofilm formation. In our experiments, we found that the sub-MIC concentration of ZnO NPs increased the gene expression of antitoxins mazE and mqsA and toxin higB of TA systems treated with ZnO NPs. Conclusions In the present study, ZnO NPs were shown to effectively inhibit biofilm formation in P. aeruginosa. Our results support the relationship between TA systems and ZnO NPs in biofilm formation in P. aeruginosa. Importantly, the expression of antitoxins mazE and mqsA was high after treatment with ZnO NPs, but not that of antitoxin higA.

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Assessment of biofilm formation in Pseudomonas aeruginosa by antisense mazE-PNA

Cited by 11 publications

References 8 publications

The Streptococcus pneumoniae yefM-yoeB and relBE Toxin-Antitoxin Operons Participate in Oxidative Stress and Biofilm Formation

The Streptococcus pneumoniae yefM-yoeB and relBE Toxin-Antitoxin Operons Participate in Oxidative Stress and Biofilm Formation

Bacterial toxin-antitoxin system MazEF as a native defense mechanism against RNA phages inEscherichia coli

Effect of ZnO nanoparticles on biofilm formation and gene expression of the toxin-antitoxin system in clinical isolates of Pseudomonas aeruginosa

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