Objective: The purpose of this study was to evaluate of gender effect in treatment of alveolar cleft with iliac bone graft by means of volumetric analysis. Methods: In this study; 22 patients with alveolar cleft including 13 unilateral, and 9 bilateral were treated with anterior iliac crest bone graft. Patients were divided into subgroups according to their gender. Cone Beam Computed Tomography scans were obtained preoperatively and postoperatively. 3D reconstruction of Cone Beam Computed Tomography images was made by using Mimics software. Preoperative alveolar cleft volume and postoperative newly formed bone volume was assessed volumetrically. Results and Conclusion: The percentage of preoperative alveolar cleft volume in female ranged from 0.51 to 1.45 cm3 with a mean volume of 0.89±0.27 cm3, in male ranged from 0.72 to 2.04 cm3 with a mean volume of 1.11±0.36 cm3. The percentage of newly formed bone in female ranged from 47.02% to 80.09% with a mean percentage of %68.23±%9.43, in male ranged from 50.70% to 77.49% with a mean percentage of 63.09%±10.72%. There is no statistically significant difference between the female and male for postoperative newly formed bone in treatment of alveolar cleft with iliac bone graft (p>0.05).