Assessment of butorphanol-azaperone-medetomidine combination as anesthesia for semen collection and evaluation of semen quality in white-tailed deer ( Odocoileus virginianus )

Kirschner, S.M.; Rodenkirch, R.

doi:10.1016/j.anireprosci.2017.07.016

Cited by 6 publications

(4 citation statements)

References 37 publications

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“…The measurements obtained in the current study reflect these values. Similar results were obtained for other cervids, including Dama dama, Odocoileus virginianus, Elaphodus cephalophus, C. unicolor, and M. nana (Chacur et al, 2014;de Abreu et al, 2009;Fernández, Sestelo, Rivolta, & Córdoba, 2013;Kirschner & Rodenkirch, 2017;Panyaboriban et al, 2016).…”

Section: Discussionsupporting

confidence: 83%

“…Sperm morphometry is a component of morphological analysis that is used to evaluate changes to the size of a cellular region, contributing toward determining a standard for sperm size of different species (Beracochea et al, 2014). Species‐specific morphometric description could be used to perform computer‐assisted sperm analysis of kinetic parameters and morphology (Kirschner & Rodenkirch, 2017; Yániz, Soler, & Santolaria, 2015). Measurements of M. gouazoubira sperm in the current study were similar to those described previously for this species, although tail and total length had slightly lower means in the present work.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of seminal parameters, sperm morphometry, micromorphology, and ultrastructure in gray brocket deer (Mazama gouazoubira, Fischer, 1814)

Cunha

Souza

Brito

et al. 2020

Microscopy Res & Technique

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Populations of gray brocket deer (Mazama gouazoubira) are declining; yet, knowledge on the reproductive biology of this species remains limited. Therefore, this study aimed to describe morphology, viability, membrane integrity, mitochondrial activity, morphometry, micromorphology, and ultrastructure of the gray brocket deer sperm. Three adult male gray brocket deer were used in the study. Semen collection was performed using electroejaculation. Semen were analyzed by evaluating pH, motilities, vigor, mass movement, volume, concentration, viability, membrane integrity, mitochondrial activity, morphology, and morphometry. Micromorphology and ultrastructure of sperm were analyzed using scanning and transmission electron microscopy (SEM and TEM), respectively. There was no significant difference among males regarding on pH, motilities, vigor, mass movement, volume, concentration, viability. High values for membrane integrity, mitochondrial activity, and normal sperm were observed. The most frequent defects were simple bent tail and bowed midpiece. The head length, and width, midpiece, and tail length were 8.5, 4.4, 11.5, and 41.3 μm, respectively. SEM sperm showed paddle‐shaped heads, with apical ridge and serrated band on the equatorial segment. TEM revealed the nucleus, acrosome, plasma membrane, mitochondria sheath, proximal centrioles, segmented columns, axoneme, outer dense fibers, and fibrous sheath. SEM and TEM showed the presence of some abnormalities. These results are expected to provide baseline values of diverse semen parameters, contributing toward the development of reproductive biotechnologies for gray brocket deer and, other deer species at risk of extinction.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Characterization of seminal parameters, sperm morphometry, micromorphology, and ultrastructure in gray brocket deer (Mazama gouazoubira, Fischer, 1814)

Cunha

Souza

Brito

et al. 2020

Microscopy Res & Technique

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“…General anaesthesia study has been performed successfully in various species of deer (25), and is an eminent in deer semen collection procedure. However, the study of general anaesthesia in relation to semen collection remains limited (26), though it is the only method of semen collection in untrained animals. Moreover, the recorded anaesthesia protocol in R. timorensis remains scarce (8).…”

Section: Discussionmentioning

confidence: 99%

Semen collection in Rusa timorensis under general anaesthesia by using ketamine-xylazine: Clinical study

Fitri¹,

Wahid²,

Jesse³

et al. 2021

IJVS

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“…Digital massage alone has been a successful tool in collecting semen from various species of wildlife, such as crocodiles, Bali cattle and endangered nonhuman primates [43][44][45]. In this study, general anesthesia provides better welfare to the animal as it is painless and less stressful [46]. Apart from that, the drug also has an alpha-adrenergic effect on the smooth muscle involved with semen release, allowing the flow of semen in the reproductive tract to the penile tip [47].…”

Section: Discussionmentioning

confidence: 99%

The Comparison of Semen Collection in Electroejaculation, Rectal Massage and Combination of Both Methods in the Critically Endangered Malayan Pangolin, Manis javanica

Tarmizi

Chee

Sipangkui

et al. 2020

Animals

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This article describes the semen characteristics from different collection methods between captive and confiscated Malayan pangolins, Manis javanica. Semen was collected from 15 pangolins; two captive and 13 confiscated individuals at the mean weight of 9.36 ± 1.94 kg. The three semen collection methods employed were electroejaculation, rectal massage and a combination of both techniques. The semen characteristics (mean ± standard deviation) of the Malayan pangolin are volume (73.75 ± 144.57 µL), pH (7.63 ± 0.53), spermatozoa concentration (997.19 ± 728.98 × 106 /mL), total motility (59.60% ± 30.00%), progressive motility (48.95% ± 30.93%), mass motility (3.50 ± 1.50) and live spermatozoa (80.25% ± 13.45%). There was no significant difference in semen characteristics between the three collection methods. The percentages of live spermatozoa were significantly different, suggesting better samples from captive compared to confiscated animals. However, there was no significant difference in spermatozoa kinetics between the captive and confiscated samples, suggesting the potential of utilizing confiscated individuals for gamete recovery to conserve the genetic pool of pangolins. All three methods of semen collection were successfully performed in pangolins and should be considered; however, electroejaculation remains the most consistent method of obtaining semen from the species.

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Assessment of butorphanol-azaperone-medetomidine combination as anesthesia for semen collection and evaluation of semen quality in white-tailed deer ( Odocoileus virginianus )

Cited by 6 publications

References 37 publications

Characterization of seminal parameters, sperm morphometry, micromorphology, and ultrastructure in gray brocket deer (Mazama gouazoubira, Fischer, 1814)

Characterization of seminal parameters, sperm morphometry, micromorphology, and ultrastructure in gray brocket deer (Mazama gouazoubira, Fischer, 1814)

Semen collection in Rusa timorensis under general anaesthesia by using ketamine-xylazine: Clinical study

The Comparison of Semen Collection in Electroejaculation, Rectal Massage and Combination of Both Methods in the Critically Endangered Malayan Pangolin, Manis javanica

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