Assessment of capillary volumetric blood microsampling for the analysis of central nervous system drugs and metabolites

Protti, Michele; Marasca, Camilla; Cirrincione, Marco; Cavalli, Andrea; Mandrioli, Roberto; Mercolini, Laura

doi:10.1039/d0an01039a

Cited by 29 publications

(26 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Initial performance evaluations show promise, 46,47 and the device has been employed for the measurement of fluoxetine and sertraline, and their respective main active metabolites, by LC-MS/MS. 48 Blood samples were collected from patients undergoing treatment and used to fill the device, with the spots collected for preparation. Among the usual validation parameters, the effect of Hct was tested at 0.30, 0.50, and 0.70, as well as for how long the blood drop was sampled.…”

Section: Volumetric Dbsmentioning

confidence: 99%

Microsampling Devices for Routine Therapeutic Drug Monitoring—Are We There Yet?

Morgan

2021

Therapeutic Drug Monitoring

View full text Add to dashboard Cite

Background: The use of microsampling for therapeutic drug monitoring (TDM) is increasingly feasible as sensitive methods have become more accessible. There exists an increasing interest in the use of microsampling, and new microsampling devices and techniques can potentially improve patient convenience and care, among other features. This review provides an update on currently validated methods for measuring drugs pertinent to TDM, including data from clinical samples.Methods: A literature record search was undertaken, including PubMed and Google Scholar. Reports that included the use of microsampling to measure concentrations of drugs associated with TDM were reviewed and included if data from patient samples were reported. The studies are described in brief, including sample preparation and analyte stability, with the most pertinent findings reported.Results: Sensitive analyses and innovative designs and materials have resulted in an increasing number of reported evaluations and validations for measuring drugs using microsamples. Novel designs largely overcome common problems associated with traditional dried blood spot sampling. Although examples of patient self-sampling are rare at present, studies that evaluated feedback found it to be largely positive, revealing the feasibility of microsampling for TDM. Conclusions:Microsampling is suited to the TDM of numerous drugs in diverse situations, and it will play an increasingly important role. The issues with traditional dried blood spot samples have been largely overcome by employing novel methods to obtain volumetric samples.

show abstract

Section: Volumetric Dbsmentioning

confidence: 99%

Microsampling Devices for Routine Therapeutic Drug Monitoring—Are We There Yet?

Morgan

2021

Therapeutic Drug Monitoring

View full text Add to dashboard Cite

show abstract

“…Unfortunately, this strategy does not always guarantee sampling accuracy because blood hematocrit (HCT) affects spot size and homogeneity, as well as analytical accuracy ( 18 ). To overcome the HCT-dependence of classic DBS, several technologies for volumetric blood microsampling have been proposed ( 19 , 20 ). In this research, an innovative microfluidic channel-based technology is proposed for consecutive generation of four capillary volumetric blood spots, avoiding potential HCT issues.…”

Section: Introductionmentioning

confidence: 99%

Dried Volumetric Microsampling Approaches for the Therapeutic Drug Monitoring of Psychiatric Patients Undergoing Clozapine Treatment

et al. 2022

Self Cite

View full text Add to dashboard Cite

Clozapine is one of the most widely used second-generation antipsychotic drugs (SGAs) for the treatment of schizophrenia. Despite advantages over first-generation drugs, clozapine still shows significant side effects and interindividual variations in efficacy. In order to ensure frequent therapeutic drug monitoring (TDM) and improve the compliance of psychiatric patients undergoing clozapine treatment, two novel dried microsampling approaches based on whole blood and plasma volumetric absorptive microsampling (b-VAMS and p-VAMS) and microfluidic generated-dried blood spot technology (mfDBS) were developed and coupled to HPLC with electrochemical detection (ED). The proposed miniaturized strategies by means of VAMS and microfluidic channel-based devices provide several advantages in terms of collection, storage, and handling compared to classical blood and plasma processing. Satisfactory validation results were obtained for all microsampling platforms, with mean extraction yields >85.1%, precision as relative standard deviation (RSD) < 5.1%, and stability < 4.5% analyte loss after 30 days for p-VAMS; mean extraction yields > 83.4%, precision RSD < 5.4%, and stability < 4.6% analyte loss after 30 days for b-VAMS, and mean extraction yields > 74.0%, precision RSD < 5.6%, and stability < 4.9% analyte loss after 30 days for mfDBS. The original microsampling methodologies have been successfully applied to the blood and plasma collected from five psychiatric patients for the monitoring of the levels of clozapine and its main metabolites, providing robust and reliable quali-quantitative results. Comparisons between results of the two dried microsampling technologies with those obtained by classic fluid plasma analysis were in good agreement and have demonstrated that the proposed miniaturized approaches could be suitable for TDM purposes.

show abstract

“…Blood collection was achieved using a microsampling device named hemaPEN ® (Trajan Scientific and Medical, VIC, Australia). This pen-shaped device allows the accurate and precise collection of samples from a single drop of blood and, depending on the studied compounds, is influenced in a limited way or not influenced by hematocrit compared to classical DBS ( Deprez et al, 2019 ; Protti et al, 2020 ). Blood is absorbed by four capillaries and transferred to four paper discs.…”

Section: Introductionmentioning

confidence: 99%

Blood Microsampling to Monitor Metabolic Profiles During Physical Exercise

Nix

Hemmati

Cobraiville

et al. 2021

Front. Mol. Biosci.

View full text Add to dashboard Cite

Monitoring approaches and technical improvements are key factors to improve a sportsman’s health, training, and recovery after an injury. In this study, a targeted metabolomics approach using microsampling with hemaPEN® was developed to measure changes in blood concentrations of nine amino acids and four organic acids before, during, and after exercise. The aim of this research project was to investigate if a reliable monitoring of metabolite levels during sports activity can be achieved by collecting one drop of whole blood at different time points. A hemaPEN device is an easy-to-use and noninvasive microsampling technique designed to collect four accurate and precise blood volumes simultaneously (10.96 µl). Twenty healthy volunteers between 19 and 30 years of age were included in this study. Physical activity consisted in running as fast as possible 1,600 m after 400 m warm-up. One drop of blood was collected at five time points: before exercise, after 800-m running, after 1,600 m, and 30 min and 60 min after finishing the exercise. The influence of physical activity on metabolite levels was evaluated using two ultrahigh-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) methods. Analytical performance criteria such as metabolite stability, method precision, trueness, and accuracy were found to be satisfactory. Expected significant metabolic changes were identified for lactic acid, main TCA cycle intermediates, and some amino acids (e.g., creatinine, choline, and taurine). This preliminary study performed on a small cohort demonstrated a high interest of using microsampling for fluxomics analysis, not only to collect quickly and easily biological samples during sports events but also because it is much easier to store and to process the samples than classical plasma/serum samples obtained by venipuncture. The present results open new avenue for fluxomics analysis in the context of health care.

show abstract

Assessment of capillary volumetric blood microsampling for the analysis of central nervous system drugs and metabolites

Abstract: Development of an advanced analytical method coupling capillary dried blood spots and LC-MS/MS for the TDM of patients undergoing treatment with CNS drugs.

Cited by 29 publications

References 36 publications

Microsampling Devices for Routine Therapeutic Drug Monitoring—Are We There Yet?

Microsampling Devices for Routine Therapeutic Drug Monitoring—Are We There Yet?

Dried Volumetric Microsampling Approaches for the Therapeutic Drug Monitoring of Psychiatric Patients Undergoing Clozapine Treatment

Blood Microsampling to Monitor Metabolic Profiles During Physical Exercise

Contact Info

Product

Resources

About