Assessment of CD24 expression on bone marrow neutrophilic granulocytes: CD24 Is a marker for the myelocytic stage of development

Abstract

The understanding of normal surface maturation pattern of granulocytes plays an essential role in identifying abnormal patterns, which may be of diagnostic or pathogenetic significance in some disorders, such as myclodysplastic syndromes. CD24, which is expressed only on granulocytes and not on monocytes, has not been adequately studied. Surface marker studies on eight control marrows indicate that CD24 is expressed on bone marrow granulocytes in a pattern that is similar to CD11b while much different than the more mature markers, CD16b and CD35. Three sorting experiments show that the majority of CD24 − cells are promyelocytes. We conclude that CD24 is expressed on the neutrophilic granulocytes at the stage of myelocytes, and hence it could potentially be used to study normal and abnormal maturation of granulocytes. Am. J. Hematol. 71:348-349, 2002.

Normal patterns of expression of glycosylphosphatidylinositol‐anchored proteins on different subsets of peripheral blood cells: A frame of reference for the diagnosis of paroxysmal nocturnal hemoglobinuria

“…CD157, an ectoenzyme of the CD38 family (20), could be potentially useful for the analysis of monocytes and neutrophils, CD24 for the study of neutrophils and B cells (27,28), whereas in line with our findings, CD14 and CD66b are already widely used in PNH patients to detect clonal cells among PB monocytes and neutrophils, respectively. In turn, expression of CD87 (29,30), the urokinase plasminogen activator receptor, despite having been observed on both monocytes and neutrophils, was found to be heterogeneous, overlapping with negative cells, which limits its utility for the identification of PNH cells, unless more sensitive approaches are used for its detection.…”

CD24-Triggered Caspase-Dependent Apoptosis via Mitochondrial Membrane Depolarization and Reactive Oxygen Species Production of Human Neutrophils Is Impaired in Sepsis

“…CD24 is a small mucin-like protein localized at the outer cell surface and expressed on a broad range of hematopoietic cells (12). Data from the current study define the function of CD24 on human neutrophils in both normal and inflammatory or sepsis conditions.…”

“…In hematopoietic cells, CD24 is expressed on B cells, T cells, eosinophils, dendritic cells, and, most prominently, neutrophils (12). It has been stated that CD24 expression on T cells is required for optimal homeostatic proliferation (13), whereas several other reports showed that cross-linking of CD24 transduces apoptotic signals in other cell types (14)(15)(16).…”

Quantitative analysis of the expression of glycosylphosphatidylinositol‐anchored proteins during the maturation of different hematopoietic cell compartments of normal bone marrow

“…at the myelocyte and band maturation stages, respectively (5,6); in turn CD66b and CD16 would be expressed at earlier stages of maturation, the highest expression of CD16 being observed on bands and mature neutrophils (7)(8)(9)(10)(11). CD14 expression would be restricted to the latest stages of the maturation of normal monocytic cells (12).…”