Assessment of Cellular Proliferation in Oral Verrucous Carcinoma and Well-Differentiated Oral Squamous Cell Carcinoma Using Ki67: A Non-Reliable Factor for Differential Diagnosis?

Zargaran, Massoumeh; Eshghyar, Nosratollah; Baghaei, Fariba; Moghimbeigi, Abbas

doi:10.7314/apjcp.2012.13.11.5811

Cited by 13 publications

(10 citation statements)

References 30 publications

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“…However, it is currently difficult to differentiate them by simply observing clinical and pathological features because OVC has similar biological behavior to OSCC, including tendency to local invasion, insidious lymph node metastasis and occurrence of malignant lesions (10). These similarities usually cause clinical misdiagnosis and mistreatment (11). Undoubtedly, it is critical to seek reliable molecular markers of OVC to resolve such challenges.…”

Section: Introductionmentioning

confidence: 99%

Oral verrucous carcinoma: From multifactorial etiology to diverse treatment regimens (Review)

Peng

Wang

Quan

et al. 2016

International Journal of Oncology

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Abstract. Oral verrucous carcinoma (OVC) is a verrucous variant of oral squamous cell carcinoma (OSCC), which accounts for 2-12% of all oral carcinomas with a 5-year survival rate of only approximately 50%. Enormous effort has been dedicated to this cancer, and the past decades have witnessed significant advances in relevant diagnostic and therapeutic approaches. Currently, there exist three challenges from primary sub-fields of research and clinical practice of the cancer, namely multifactorial etiology, complex molecular mechanism, and deficient treatment. This study reviews the existing literature on the cancer, encompassing its etiology, clinical manifestations and pathology, molecular mechanism, diagnosis and differential diagnosis, and treatment. For improved treatment of OVC, multifactorial etiology analysis, incorporation of effective biomarkers for mechanism illustration, and integration of multidisciplinary modalities are expounded, in an attempt to resolve the challenges and to provide a useful guide for future research in the field.

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Section: Introductionmentioning

confidence: 99%

Oral verrucous carcinoma: From multifactorial etiology to diverse treatment regimens (Review)

Peng

Wang

Quan

et al. 2016

International Journal of Oncology

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“…1 In addition to this, the studying of the histopathological profiles of the cancer cell has largely remained subjective, embodying a possibility of individual bias toward mendacious interpretation of the disease. Therefore, a genuine reason thus culminated to find out an objective guideline that would attribute collectively and resultantly for an absolutely clear diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Proliferative Index in Invasive Tumor Front of Oral Squamous Cell Carcinoma: A Potential Prognostic Indicator

Bhuyan¹,

Sarangi²,

Das³

et al. 2018

The Journal of Contemporary Dental Practice

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Aim:The aim of the study was to evaluate the proliferative index (PI) at their invasive front of oral cancer and their association with Bryne's grades of oral squamous cell carcinoma (OSCC) and compare the PI with lymph node metastasis, site of involvement, and habits. Materials and methods:The Ki-67 antigen expression was immunohistochemically evaluated in a total of 102 cases that included the histopathologically diagnosed archival specimens of OSCCs. They were subdivided by Bryne's histopathological grading into grade I (40 cases), grade II (32 cases), and grade III (30 cases). The nucleus with brown stain was considered positive. Cells were counted under 400× magnification. The proliferative activity thus determined was then expressed as a percentage of Ki-67 labeling index (Ki-67 LI) positive cells. Results:A stepwise increase in the mean Ki-67 LI was found from grade I to III squamous cell carcinoma, thus correlating with the histological grading. In addition, there was a higher PI seen in cases associated with metastatic lymph node, which concords with the higher biologic aggressiveness and poor prognosis of the lesion. Conclusion:The present study shows a definitive correlation of Ki-67 antigen with the Bryne's histological grading, all the parameters of Bryne's grading for OSCC and lymph node status of the patient proving its association as an effective tool to grade the tumors and finally read the prognosis of the tumor. Clinical significance: Cell proliferation is regarded as one of the most important biologic mechanisms in oncogenesis. The role of cell proliferation in tumor progression has been inferred in studies concerned with human cancer by comparing the PI of normal tissue, preneoplastic and neoplastic lesions. The Ki-67 antigen-labeled cells can prove to be an effective aid to grade the tumors. It might be possible to standardize and objectify tumor grading among pathology laboratories. Proliferative Index in Invasive Tumor

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“…This suggests that OVC is characterised by a lower degree of chromosomal instability than OSCC. This lower level of chromosomal instability could be linked to the minimal or absent histological cytological atypia found in OVC . We used logistic regression analysis to obtain statistical quantitation of the CN differences, correctly identifying 56 of 57 OVC samples and 41 of 45 OSCCs.…”

Section: Discussionmentioning

confidence: 99%

A novel genomic signature reclassifies an oral cancer subtype

Samman

Wood

Conway

et al. 2015

Intl Journal of Cancer

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Verrucous carcinoma of the oral cavity (OVC) is considered a subtype of classical oral squamous cell carcinoma (OSCC). Diagnosis is problematic, and additional biomarkers are needed to better stratify patients. To investigate their molecular signature, we performed low-coverage copy number (CN) sequencing on 57 OVC and exome and RNA sequencing on a subset of these and compared the data to the same OSCC parameters. CN results showed that OVC lacked any of the classical OSCC patterns such as gain of 3q and loss of 3p and demonstrated considerably fewer genomic rearrangements compared to the OSCC cohort. OVC and OSCC samples could be clearly differentiated. Exome sequencing showed that OVC samples lacked mutations in genes commonly associated with OSCC (TP53, NOTCH1, NOTCH2, CDKN2A and FAT1). RNA sequencing identified genes that were differentially expressed between the groups. In silico functional analysis showed that the mutated and differentially expressed genes in OVC samples were involved in cell adhesion and keratinocyte proliferation, while those in the OSCC cohort were enriched for cell death and apoptosis pathways. This is the largest and most detailed genomic and transcriptomic analysis yet performed on this tumour type, which, as an example of non-metastatic cancer, may shed light on the nature of metastases. These three independent investigations consistently show substantial differences between the cohorts. Taken together, they lead to the conclusion that OVC is not a subtype of OSCC, but should be classified as a distinct entity.

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Assessment of Cellular Proliferation in Oral Verrucous Carcinoma and Well-Differentiated Oral Squamous Cell Carcinoma Using Ki67: A Non-Reliable Factor for Differential Diagnosis?

Abstract: -1) is not a good immunohistochemical marker to assess invasion status and differentiate OVC from well-differentiated OSCC; also, it cannot be used as a diagnostic tool to distinguish between variants of OSCC with similar grade.

Cited by 13 publications

References 30 publications

Oral verrucous carcinoma: From multifactorial etiology to diverse treatment regimens (Review)

Oral verrucous carcinoma: From multifactorial etiology to diverse treatment regimens (Review)

Proliferative Index in Invasive Tumor Front of Oral Squamous Cell Carcinoma: A Potential Prognostic Indicator

A novel genomic signature reclassifies an oral cancer subtype

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