Assessment of Centella Asiatica Extract Containing Dual-crosslinked Gel-MA/Pec Hydrogels as Wound Dressing

Abstract

In the present work, 3D-printed wound dressings containing different amounts of Centella Asiatica extract were synthesized via dual-crosslinking method. Methacrylic anhydride modified gelatin (Gel-MA) and pectin (Pec) were chosen as the base material for dressings. A dual crosslinked network was formed with Gel-MA photo-crosslinking using Irgacure 2959 and Pectin (Pec) psychical-crosslinking using Ca++ ions. Meanwhile, the developed dual-crosslinked hydrogel dressings were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, and mechanical, swelling, in vitro degradation as well as ex vivo bioadhesion tests. It has been observed that the preapred dressings provide good bioadhesion on the chicken skin. Moreover, MTT assay revealed cell viability of Gel-MA/Pec hydrogel dressings containing Centella Asiatica extract. The results presented the dressings as a promising biomaterial to be used for wound healing purposes, which should be further investigated in future.

Novel design and development of Centella Asiatica extract - loaded poloxamer/ZnO nanocomposite wound closure material to improve anti-bacterial action and enhanced wound healing efficacy in diabetic foot ulcer

“…Wound dressings formulated with CAE have an advantage in healing because it promotes collagen synthesis and fibroblast proliferation [ [22] , [23] , [24] ]. As a result, several biomaterials have been studied in relation to CAE's potential use as a wound dressing [ [25] , [26] , [27] ]. As a result, there is growing interest in using CAE in wound dressing applications, and a number of biomaterials have been studied [ 28 , 29 ].…”