Assessment of Chromogenic Media in Bacterial Fish Pathogens

Abstract

Accurate and quick identification of bacterial fish disease has great importance for effective disease control and prevent economic loss in aquaculture facilities. Generally used agar media allow the growth of wide-range bacteria but new chromogenic media results in colored colonies that lead to presumptive identification without requiring biochemical or serological tests. In this study, the recently commercialized chromogenic media; chromID ® CPS ® Elite, chromID ® CPS ® Elite / Columbia CNA +5% sheep blood agar, chromID ® Vibrio, chromID ® MRSA Smart and chromID ® Staphylococcus aureus Elite (BioMérieux, France) were tested with growth performance and colony coloration of bacterial fish pathogens. chromID ® CPS ® Elite and chromID ® CPS ® Elite / Columbia CNA +5% sheep blood agar were detected the most suitable culture media to grow for all five isolated fish pathogens (i.e., Aeromonas hydrophila, Lactococcus garvieae, Pseudomonas fluorescens, Yersinia ruckeri and Vibrio anguillarum). Of which, L. garvieae was the only one observed with green colonies. chromID ® Vibrio designed for V. parahaemolyticus and V cholerae was lead dark pink/purple colonies by V. anguillarum. The chromID ® MRSA Smart and chromID ® S. aureus Elite were detected not effective for incubation of bacterial fish pathogens.