Assessment of Chromosomal Aberrations and Micronuclei in Peripheral Lymphocytes from Tunisian Hospital Workers Exposed to Ionizing Radiation

Sakly, Amina; Ayed, Yosra; Chaari, Néila; Akrout, Mohamed; Bacha, Hassen; Cheikh, Hassen Ben

doi:10.1089/gtmb.2012.0111

Cited by 21 publications

(18 citation statements)

References 42 publications

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“…Our study established that the incidence of MN significantly increased for health workers in nuclear medicine departments occupationally exposed to ionizing radiation in comparison to unexposed individuals. Our findings are in accordance with the most other literature data [ 15 – 17 ]. The novelty of this study, that sets it apart from other similar investigations, is construction of a new model for prediction of micronuclei formation in nuclear medicine department workers professionally exposed to ionizing radiation.…”

Section: Discussionsupporting

confidence: 94%

New models for prediction of micronuclei formation in nuclear medicine department workers

Terzić¹,

Milovanović

Dotlić

et al. 2015

J Occup Med Toxicol

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BackgroundIonizing radiation causes detrimental health effects such as cancer and genetic damage. The study aim was to determine predictors for micronuclei (MN) occurrence and frequency in peripheral blood lymphocytes of health workers professionally exposed to radiation.MethodsHealth workers, age matched, selected for the study on regular check-ups, were divided according to the radiation exposure. The exposed group involved nuclear medicine department employees (54) and the control group comprised workers from other departments (36). Data about workers characteristics and habits, received annual doses (AD), total years of service (TYS) and exposed years of service (EYS) were taken from each subject. Blood samples were taken and micronuclei (MN) number in peripheral blood lymphocytes was calculated using CBMN assay according to standard protocols.ResultsMost workers were female, technicians, with mean age of 45.67 years and EYS about 15 years. Health workers exposed to radiation had significantly more MN than controls (p = 0.001). Female gender, older age, higher received annual doses, longer EYS and TYS increased the MN number. Technicians and laboratory workers have higher risk for MN occurrence. Significant predictors of MN formation according to constructed model were workers age, sex, AD and EYS. One EYS year increases MN frequency 1.017 times, while receiving 0.1 mSy raises MN frequency by 26 %. EYS accurately predicts 86.30 % of MN frequencies and AD 64.60 %.ConclusionsThe model, developed for the first time in this study, showed that received annual doses and duration of exposure to radiation can be used for prediction of MN numbers.

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Section: Discussionsupporting

confidence: 94%

New models for prediction of micronuclei formation in nuclear medicine department workers

Terzić¹,

Milovanović

Dotlić

et al. 2015

J Occup Med Toxicol

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“…study the mean frequencies of micronuclei in the 30 cardiology department workers (25.57-4.79‰) and 30 radiology department workers (21.90-4.23‰) were significantly higher than that in the 27 controls (10.78-1.47‰) (Sakly et al, 2013). The MN frequency in the 67 healthcare workers occupationally exposed to ionizing radiation below the permissible dose of 50 mSv/year showed a significant higher MN frequency compared to the control group (13.63 ± 4.9‰ vs. 6.52 ± 4.21‰, p < 0.05).…”

Section: Resultsmentioning

confidence: 87%

Occupational radiation exposure and genetic polymorphisms in DNA repair genes

Zakeri

Farshidpour²,

Rajabpour³

2017

Radioprotection

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Radiation sensitivity appears to be influenced by genetic polymorphisms in some DNA repair genes and individuals with low DNA repair capacity can be at increased risk of developing cancer. The aim of the present study was to determine the impact of gene polymorphisms on DNA damages in the form of micronuclei (MN) in circulating lymphocytes of interventional cardiologists (ICs), occupationally exposed to ionizing radiation. MN frequency was significantly higher in the ICs than in the controls (27.7 ± 17.2‰ vs. 8.0 ± 2.6‰, p < 0.0005). MN values were higher in the ICs with exposure of ≥ 10 years and ≥ 3 mSv in comparison to the related referents. The analysis of different interactions showed significantly higher MN frequencies in ICs exposed to ≥ 10 mSv carrying the variant-type XRCC3 241Met genotype compared to those of wild-type XRCC3 (Thr/Thr) genotype. In the ICs carrying both wild-type alleles for XRCC3 and MSH3 polymorphisms showed a significantly lower MN frequency compared to the exposed individuals with one or two variant alleles for XRCC3 and MSH3 polymorphisms. In conclusion, ICs showed significantly higher MN frequencies than in the control, and this effect was higher in individuals carrying the variant-type XRCC3 241Met genotype. These results not only show the need for improvement of safety and training programs for ICs but also may be helpful in developing amenable screening tests for identifying radiosensitive workers and applying suitable radiation protection job-specific strategies for effective dose reduction.

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“…Indeed, most of the occupational exposures are below the lowest resolution power observed in ex vivo cytogenetic assays [ 92 , 93 , 94 ], but previous studies did show a significant increase in cytogenetic endpoints in patients who received radiation exposure during a single medical examination, such as a CT scan or catheterization procedure, where the radiation dose is far lower than 100 mSv [ 95 , 96 , 97 ]. Similarly the effect of confounding factors on CA frequencies was inconsistent between studies, with some authors reporting the effect of certain factors such as age, smoking and gender [ 54 , 57 , 60 , 72 ], while others showing no influence [ 62 , 64 , 76 , 87 ]. Furthermore, statistically significant differences in CA frequencies were reported between cohorts employed at different working places, with specificity of certain types of CA depending on the job.…”

Section: Discussionmentioning

confidence: 99%

Biomarkers of Genotoxicity in Medical Workers Exposed to Low-Dose Ionizing Radiation: Systematic Review and Meta-Analyses

Baudin¹,

Bernier²,

Klokov

et al. 2021

IJMS

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Medical staff represent the largest group of workers occupationally exposed to ionizing radiation (IR). Chronic exposure to low-dose IR may result in DNA damage and genotoxicity associated with increased risk of cancer. This review aims to identify the genotoxicity biomarkers that are the most elevated in IR-exposed vs. unexposed health workers. A systematic review of the literature was performed to retrieve relevant studies with various biomarkers of genotoxicity. Subsequent meta-analyses produced a pooled effect size for several endpoints. The search procedure yielded 65 studies. Chromosome aberrations (CA) and micronuclei (MN) frequencies were significantly different between IR-exposed and unexposed workers (θpooled = 3.19, 95% CI 1.46–4.93; and θpooled = 1.41, 95% CI 0.97–1.86, for total aberrant cells and MN frequencies, respectively), which was not the case for ring chromosomes and nucleoplasmic bridges. Although less frequently used, stable translocations, sister chromatid exchanges (SCE) and comet assay endpoints were also statistically different between IR-exposed and unexposed workers. This review confirms the relevance of CA and MN as genotoxicity biomarkers that are consistently elevated in IR-exposed vs. unexposed workers. Other endpoints are strong candidates but require further studies to validate their usefulness. The integration of the identified biomarkers in future prospective epidemiological studies is encouraged.

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Assessment of Chromosomal Aberrations and Micronuclei in Peripheral Lymphocytes from Tunisian Hospital Workers Exposed to Ionizing Radiation

Cited by 21 publications

References 42 publications

New models for prediction of micronuclei formation in nuclear medicine department workers

New models for prediction of micronuclei formation in nuclear medicine department workers

Occupational radiation exposure and genetic polymorphisms in DNA repair genes

Biomarkers of Genotoxicity in Medical Workers Exposed to Low-Dose Ionizing Radiation: Systematic Review and Meta-Analyses

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