Assessment of Conjugate Complexes of Chitosan and Urtica dioica or Equisetum arvense Extracts for the Control of Grapevine Trunk Pathogens

Langa-Lomba, Natalia; Buzón-Durán, Laura; Martín-Ramos, Pablo; Casanova-Gascón, José; Martín‐Gil, Jesús; Sánchez-Hernández, Eva; García, Vicente González

doi:10.3390/agronomy11050976

Cited by 25 publications

(23 citation statements)

References 60 publications

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“…The bioactive constituents present in each of the extracts, characterized by gas chromatography-mass spectroscopy (GC-MS), have been reported in previous works [19][20][21].…”

Section: Preparation Of Plant Extractsmentioning

confidence: 97%

“…After cooling, the decoction was filtered and diluted by a factor of 10 with water to obtain a final concentration of 2 mg•mL −1 . For the nettle extract, dried nettle leaves (15 g•L −1 ) were macerated for 3-4 days at 20 • C, followed by filtering and dilution of the filtrate to obtain a final concentration of 2 mg•mL −1 [19].…”

Section: Preparation Of Plant Extractsmentioning

confidence: 99%

“…Simmons [18]. As an alternative, this work proposes the use of plant extracts for which inhibitory concentrations below 1000 ppm against Botryosphaeriaceae species have been previously reported by our group, such as Rubia tinctorum L., Silybum marianum (L.) Gaertn., Urtica dioica L., or Equisetum arvense L. [19][20][21], and which have proven effective for pruning wound protection applications. These products of natural origin would be suitable for use in organic or conventional agriculture (in fact, the latter two are 'basic substances' under the EU plant protection products regulation, Article 23 of (EC) No 1107/2009).…”

Section: Introductionmentioning

confidence: 99%

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Lignin–Chitosan Nanocarriers for the Delivery of Bioactive Natural Products against Wood-Decay Phytopathogens

et al. 2022

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The use of nanocarriers (NCs), i.e., nanomaterials capable of encapsulating drugs and releasing them selectively, is an emerging field in agriculture. In this study, the synthesis, characterization, and in vitro and in vivo testing of biodegradable NCs loaded with natural bioactive products was investigated for the control of certain phytopathogens responsible for wood degradation. In particular, NCs based on methacrylated lignin and chitosan oligomers, loaded with extracts from Rubia tinctorum, Silybum marianum, Equisetum arvense, and Urtica dioica, were first assayed in vitro against Neofusicoccum parvum, an aggressive fungus that causes cankers and diebacks in numerous woody hosts around the world. The in vitro antimicrobial activity of the most effective treatment was further explored against another fungal pathogen and two bacteria related to trunk diseases: Diplodia seriata, Xylophilus ampelinus, and Pseudomonas syringae pv. syringae, respectively. Subsequently, it was evaluated in field conditions, in which it was applied by endotherapy for the control of grapevine trunk diseases. In the in vitro mycelial growth inhibition tests, the NCs loaded with R. tinctorum resulted in EC90 concentrations of 65.8 and 91.0 μg·mL−1 against N. parvum and D. seriata, respectively. Concerning their antibacterial activity, a minimum inhibitory concentration of 37.5 μg·mL−1 was obtained for this treatment against both phytopathogens. Upon application via endotherapy on 20-year-old grapevines with clear esca and Botryosphaeria decay symptoms, no phytotoxicity effects were observed (according to SPAD and chlorophyll fluorescence measurements) and the sugar content of the grape juice was not affected either. Nonetheless, the treatment led to a noticeable decrease in foliar symptoms as well as a higher yield in the treated arms as compared to the control arms (3177 vs. 1932 g/arm), suggestive of high efficacy. Given the advantages in terms of controlled release and antimicrobial product savings, these biodegradable NCs loaded with natural extracts may deserve further research in large-scale field tests.

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“…The bioactive constituents present in each of the extracts, characterized by gas chromatography-mass spectroscopy (GC-MS), have been reported in previous works [19][20][21].…”

Section: Preparation Of Plant Extractsmentioning

confidence: 97%

Section: Preparation Of Plant Extractsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Lignin–Chitosan Nanocarriers for the Delivery of Bioactive Natural Products against Wood-Decay Phytopathogens

et al. 2022

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“…Nonetheless, none of the aforementioned combinations are conjugated complexes, and the existence of interactions between the two components in terms of antimicrobial activity was not explored. Albeit for other phytochemicals different from the ones present in L. binervosum, a synergistic behavior upon conjugation with COS was reported in the literature against phytopathogens: e.g., for horsetail (Equisetum arvense L.) and nettle (Urtica dioica L.) extracts against eight fungal species involved in grapevine trunk diseases [68], with EC 90 values in the 208-1000 µg•mL −1 range (depending on the extract and on the Botryosphaeriaceae taxa). The value reported in this work for the COS-flower extract complex (914 µg•mL −1 ) would be on the upper limit.…”

Section: On the Synergistic Behavior Observed For The Conjugate Complexesmentioning

confidence: 98%

Characterization and Antimicrobial Activity of a Halophyte from the Asturian Coast (Spain): Limonium binervosum (G.E.Sm.) C.E.Salmon

Sánchez-Hernández

Buzón-Durán

Langa-Lomba

et al. 2021

Plants

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The work presented herein deals with the characterization and valorization of a halophyte from the cliffs of the Asturian coast: Limonium binervosum (G.E.Sm.) C.E.Salmon (rock sea-lavender). Its biomass and hydromethanolic extracts were studied by elemental and thermal analysis, infrared spectroscopy and gas chromatography–mass spectroscopy. Tetradecanoic acid/esters and 1,2-tetradecanediol were identified in its flower extract, while the leaf extract was rich in linolenic and linoleic acids and their esters, hexadecanoic acid and its esters, and phytol. Both flower and leaf hydromethanolic extracts contained eicosane, sitosterol and tocopherols in significant amounts. With a view to its valorization, the antimicrobial activity of these extracts was investigated against three apple tree and grapevine phytopathogens. Both the hydroalcoholic extracts and their main constituents, alone or in combination with chitosan oligomers (COS), were tested in vitro. A remarkable antibacterial activity was observed for the conjugated complexes of the flower extract with COS, both against Xylophilus ampelinus (MIC = 250 μg·mL−1) and Erwinia amylovora (MIC = 500 μg·mL−1), and complete inhibition of the mycelial growth of Diplodia seriata was found at concentrations <1000 μg·mL−1. In view of these results, this extremophile plant can be put forward as a promising source of bioactive metabolites.

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“…They point out the relationship of pathogen density and effectiveness of the basic substance to hamper the pathogen. Langa-Lomba et al (2021) found synergistic effects, while using chitosan-polymers in combination with an E. arvenseextract. For a phytosanitary programme, these approaches, the combination of copper or chitosan with E. arvenseextract can compensate for the expected lack of efficiency between a basic substance and a conventional or chemical plant protection product.…”

Section: Discussionmentioning

confidence: 95%

Potential of basic substances in plant protection to reduce Podosphaera pannosa in cut roses

et al. 2022

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Alternative strategies in plant protection are required due to political and environmental conditions. Basic substances can potentially benefit for modern plant protection in conventional and organic horticulture. In the case of plant protection in ornamentals, little is known about the effect of basic substances within the canopy to reduce foliar diseases. Podosphaera pannosa is one of the most severe diseases in the production of cut roses. In this study, different cultivars of cut roses were sprayed weekly with an extract of Equisetum arvense (4 g dried plant material/L), an aqueous solution of hydrogen peroxide (10 g/L) and a commercial plant protection product containing potassium hydrogen carbonate (VitiSan, Biofa GmbH, Münsingen, Germany) during two experimental trials. As a result, the symptoms of infected leaf area could be reduced significantly as the experiments progressed. Furthermore, no negative effects such as a reduction of plant growth or the number of marketable flowers were observed. The results indicate that the foliar application of the basic substances hydrogen peroxide and E. arvense-extract has the potential to reduce the use of synthetic plant protection products in conventional and organic horticulture.

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Assessment of Conjugate Complexes of Chitosan and Urtica dioica or Equisetum arvense Extracts for the Control of Grapevine Trunk Pathogens

Cited by 25 publications

References 60 publications

Lignin–Chitosan Nanocarriers for the Delivery of Bioactive Natural Products against Wood-Decay Phytopathogens

Lignin–Chitosan Nanocarriers for the Delivery of Bioactive Natural Products against Wood-Decay Phytopathogens

Characterization and Antimicrobial Activity of a Halophyte from the Asturian Coast (Spain): Limonium binervosum (G.E.Sm.) C.E.Salmon

Potential of basic substances in plant protection to reduce Podosphaera pannosa in cut roses

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