2014
DOI: 10.1371/journal.pone.0104620
|View full text |Cite
|
Sign up to set email alerts
|

Assessment of Cultivation Factors that Affect Biomass and Geraniol Production in Transgenic Tobacco Cell Suspension Cultures

Abstract: A large-scale statistical experimental design was used to determine essential cultivation parameters that affect biomass accumulation and geraniol production in transgenic tobacco (Nicotiana tabacum cv. Samsun NN) cell suspension cultures. The carbohydrate source played a major role in determining the geraniol yield and factors such as filling volume, inoculum size and light were less important. Sucrose, filling volume and inoculum size had a positive effect on geraniol yield by boosting growth of plant cell c… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
25
0

Year Published

2015
2015
2023
2023

Publication Types

Select...
5
4

Relationship

1
8

Authors

Journals

citations
Cited by 36 publications
(25 citation statements)
references
References 37 publications
0
25
0
Order By: Relevance
“…An alternative commonly used in industrial applications are orthogonal (Taguchi) arrays (Roy, 2010), which allow the main effects of a large number of factors to be estimated with a limited number of treatments. Although orthogonal arrays have been successfully applied to address the problem of determining adequate combinations of factors in biological and biotechnological processes (Rao et al, 2008;Assemi et al, 2012;Sedghi et al, 2014;Vasilev et al, 2014), their use for establishing seed germination protocols has been highly limited (Wu et al, 2011;Poinapen et al, 2013) and largely overlooked.…”
Section: Introductionmentioning
confidence: 99%
“…An alternative commonly used in industrial applications are orthogonal (Taguchi) arrays (Roy, 2010), which allow the main effects of a large number of factors to be estimated with a limited number of treatments. Although orthogonal arrays have been successfully applied to address the problem of determining adequate combinations of factors in biological and biotechnological processes (Rao et al, 2008;Assemi et al, 2012;Sedghi et al, 2014;Vasilev et al, 2014), their use for establishing seed germination protocols has been highly limited (Wu et al, 2011;Poinapen et al, 2013) and largely overlooked.…”
Section: Introductionmentioning
confidence: 99%
“…An example from plant biotechnology Vasilev et al (2014) investigated cultivation factors for geraniol production by plant cells. There were four quantitative 2-level factors, two 3-level factors (one quantitative and one qualitative) and one qualitative 4-level factor.…”
Section: Inspection Methods For Factorial Designsmentioning
confidence: 99%
“…Among sucrose, glucose and D -mannitol, the use of sucrose led to the highest geraniol yield and biomass accumulated in the cell cultures. Light, which can promote the synthesis of carbohydrates by photosynthesis, had also a potent effect on geraniol biosynthesis (Vasilev et al, 2014). Overexpressing the neutral/alkaline invertase gene ( NINV ), a key gene of sucrose hydrolysis, can significantly enhance the expression level of taxadiene synthase gene ( TAS ) in T. chinensis cells.…”
Section: Plant Metabolic Engineering Strategies For Production Of Phamentioning
confidence: 99%