Assessment of cytogenetic damage in bovine peripheral lymphocytes exposed to in vitro tebuconazole-based fungicide

Šiviková, Katarı́na; Dianovský, Ján; Holečková, Beáta; Galdíková, Martina; Kolesárová, Viera

doi:10.1016/j.chemosphere.2013.04.001

Cited by 33 publications

(10 citation statements)

References 41 publications

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“…As a triazole fungicide, TEB has been widely used in agriculture, leading to adverse effects on humans and wildlife (Sivikova, Dianovsky, Holeckova, Galdikova, & Kolesarova, ; Renzo et al, 2011; Castro et al, ; Bernardes, Andrade‐Vieira, Aragao, Ferreira, & Ferreira, ; Mercadante et al, ). The persistence in the environment and long half‐life mean that transgenerational investigation by parental exposure becomes very significant.…”

Section: Discussionmentioning

confidence: 99%

Transgenerational reproductive and developmental toxicity of tebuconazole in Caenorhabditis elegans

Liu

2020

J of Applied Toxicology

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The transgenerational reproductive and developmental toxicity of tebuconazole (TEB) in Caenorhabditis elegans was investigated over five generations (P0 − F4). Only parental C.elegans (P0) were exposed to TEB (0, 0.01, 0.1, 1, and 10 μg/L) for 24 h and the subsequent offspring (F1-F4) were grown under TEB-free conditions. TEB exposure caused dose-dependent reproductive defects and developmental impairments in C.elegans. In the P0 generation reproductive defects were observed such as:reduced brood size and embryo hatchability, prolonged generation time, retarded gonadal development, and slower germline proliferation, even at 0.01 μg/L, together with developmental toxicity with significant reduced body length and narrowed body width at 10 μg/L. Additionally, the brood size significantly reduced in F2, which began to recover from F3, but was still lower than the control in F4. The proportion of abnormalities increased significantly in F2 and reduced from F3, but was still higher than the control, suggesting that TEB could have cumulative potential and be passed to offspring through parental exposure. Furthermore, exposure to TEB (10 μg/L) in P0 significantly reduced the body length in F1, which began to recover from F2, and was the same level as the control in F4. There was a concentrationdependent increase in body width in F1-F4, with a significant increase only observed in F1 at 10 μg/L. Thus, parental exposure to TEB induced transgenerational defects in both reproduction and development, emphasizing the significance of considering bio-toxicity over multiple generations to conduct accurate assessment of environmental risks of toxicants. K E Y W O R D SCaenorhabditis elegans, parental exposure, reproductive and developmental toxicity, Tebuconazole, trans-generation

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Section: Discussionmentioning

confidence: 99%

Transgenerational reproductive and developmental toxicity of tebuconazole in Caenorhabditis elegans

Liu

2020

J of Applied Toxicology

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“…Given its wide uses and environmental persistence, potential human and environmental health effects of tebuconazole have received increasing attention. Toxicological studies have shown that tebuconazole increased chromosomal aberration and sister chromatid exchange in bovine peripheral lymphocytes and caused malformations at the development of branchial arches in rat embryos in vitro . Dietary tebuconazole treatment induced hypertrophy, eosinophilic altered foci, and adenomas in mouse liver .…”

Section: Introductionmentioning

confidence: 99%

Effects of tebuconazole on cytochrome P450 enzymes, oxidative stress, and endocrine disruption in male rats

Yang

Liu

Liao

et al. 2018

Environmental Toxicology

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The major objective of the present study was to determine the ability of a triazole fungicide tebuconazole to induce cytochrome P450-dependent monooxygenases, oxidative stress, and endocrine-disrupting activity using male rats treated with tebuconazole at 10, 25, and 50 mg/kg p.o. once daily for 28 days. In liver, tebuconazole dose-dependently increased microsomal contents of cytochrome P450 and cytochrome b and the activities of NADPH-cytochrome P450 reductase, 7-ethoxyresorufin O-deethylase, methoxyresorufin O-demethylase, pentoxyresorufin O-dealkylase, 7-ethoxycoumarin O-deethylase, aniline hydroxylase, and erythromycin N-demethylase. In kidney, tebuconazole increased 7-ethoxycoumarin O-deethylase activity without affecting other monooxygenase activities. In marked contrast to liver and kidney, tebuconazole decreased testicular 7-ethoxyresorufin O-deethylase, methoxyresorufin O-demethylase, 7-ethoxycoumarin O-deethylase, aniline hydroxylase, and erythromycin N-demethylase activities. The results of immunoblot analysis of liver microsomes of controls and tebuconazole-treated rats revealed that tebuconazole induced CYP1A1/2, CYP2B1/2, CYP2E1, and CYP3A proteins in liver. Additions of tebuconazole to liver microsomes inhibited microsomal 7-ethoxycoumarin O-deethylase activity in vitro (IC = 1.50-1.69 µM). Treatment of rats with tebuconazole decreased glutathione content and increased glutathione S-transferase, superoxide dismutase, catalase, and glutathione peroxidase activities in liver; increased superoxide dismutase activities in kidney and testis; but decreased glutathione S-transferase activity in testis. Treatments with tebuconazole decreased serum testosterone concentration and cauda epididymal sperm count. The present study demonstrates that tebuconazole induces a multiplicity of CYPs and oxidative stress in liver; inhibits testicular P450 and glutathione S-transferase activities; and produces anti-androgenic effects in male rats.

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“…Tebuconazole belongs to the triazole group of biocides and is used for the control of mildew, rust, net blotch, root rot, and seed-borne diseases. The most commonly available formulations are emulsifiable concentrates and wettable powders due to the low solubility in water (Šiviková et al 2013;Zhang et al 2015). Tebuconazole exhibits moderate toxicity to freshwater fish and a half-life in soil equal to 49-610 days under aerobic conditions (Bernabò et al 2016;Muñoz-Leoz et al 2011) The main action mode is the inhibition of the 14α-demethylase in ergosterol biosynthesis, an important component in fungal cell membranes, by influencing the cytochrome P450 enzyme activity (Ngo et al 2016;Bordagaray et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Dicationic triazolium fungicidal ionic liquids with herbicidal properties

et al. 2019

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The purpose of the present study was to synthesize the dicationic ionic liquids exhibiting herbicidal and fungicidal activities. A homologue series of ten dicationic ionic liquids was synthesized using commercial triazole fungicide (tebuconazole) and evaluated in terms of their possible antifungal and herbicidal activities. Dicationic triazolium compounds containing different linkers were prepared via acid-base reaction between the corresponding dicationic hydroxides and herbicides in the form of free acids. Additionally, the ionic liquids were fully characterized using NMR and thermal analysis. The herbicidal efficacy was tested during greenhouse experiments, using lambsquarters and oilseed rape as the test plants. In the case of determination of antifungal activity, six fungal species were used: Sclerotinia sclerotiorum, Botrytis cinerea, Fusarium culmorum, Fusarium oxysporum, Colletotrichum sp. and Monographella nivalis. The obtained results indicate that the conversion of compounds into dicationic ionic liquids affects their fungicidal and herbicidal activity. In the case of tests carried out on fungi, no difference in activity was observed at higher doses, except Monographella nivalis. However, at lower concentration (10 ppm), there is a reduction in efficacy compared to the reference substance. In addition, all obtained compounds are characterized by high activity.

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Assessment of cytogenetic damage in bovine peripheral lymphocytes exposed to in vitro tebuconazole-based fungicide

Cited by 33 publications

References 41 publications

Transgenerational reproductive and developmental toxicity of tebuconazole in Caenorhabditis elegans

Transgenerational reproductive and developmental toxicity of tebuconazole in Caenorhabditis elegans

Effects of tebuconazole on cytochrome P450 enzymes, oxidative stress, and endocrine disruption in male rats

Dicationic triazolium fungicidal ionic liquids with herbicidal properties

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