Assessment of density gradient centrifugation (DGC) and sperm chromatin dispersion (SCD) measurements in couples with male factor infertility undergoing ICSI

Abstract

DGC highly significantly reduces sperm DNA fragmentation in the semen of ICSI patients, with the exception of those with severe oligozoospermia. The results of the SCD test of sperm DNA fragmentation in native or DGC semen do not correlate with the fertilization rate, implantation rate, or clinical pregnancy rate in patients undergoing ICSI.

Sperm DNA fragmentation index influences assisted reproductive technology outcome: A systematic review and meta‐analysis combined with a retrospective cohort study

“…The size of the samples varied from 18 (Greco et al, ) to 1633 (Oleszczuk, Giwercman, & Bungum, ). Studies of the miscarriage rate included 25 studies comprising 3,992 couples, in which our unpublished data of 1,333 cycles were included (Anifandis et al, ; Benchaib et al, ; Boe‐Hansen, Fedder, Ersboll, & Christensen, ; Borini et al, ; Bungum et al, ; Chi et al, ; Esbert et al, ; Frydman et al, ; Gandini et al, ; Greco et al, ; Jiang et al, ; Lin et al, ; Morris, Ilott, Dixon, & Brison, ; Ni et al, ; Niu et al, ; Oleszczuk et al, ; Simon et al, ; Sivanarayana et al, ; Speyer et al, ; Virro, Larson‐Cook, & Evenson, ; Wang et al, ; Xue et al, ; Yang et al, ; Zheng et al, ). Studies of the rate of good‐quality embryos included eight studies comprising 17,879 embryos (Chi et al, ; Fang, Lou, Ye, Jin, & Zhou, ; Jiang, He, Wang et al, ; Jiang et al, ; Niu et al, ; Oleszczuk et al, ; Xue et al, ).…”

“…Studies of the rate of good‐quality embryos included eight studies comprising 17,879 embryos (Chi et al, ; Fang, Lou, Ye, Jin, & Zhou, ; Jiang, He, Wang et al, ; Jiang et al, ; Niu et al, ; Oleszczuk et al, ; Xue et al, ). Studies of the clinical pregnancy rate included 23 studies comprising 6,771 cycles (Al Omrani et al, ; Anifandis et al, ; Boe‐Hansen et al, ; Bungum et al, ; Chi et al, ; Esbert et al, ; Fang et al, ; Gandini et al, ; Jiang, He, Wang et al, ; Jiang et al, ; Lin et al, ; Miciński, Pawlicki, Wielgus, Bochenek, & Tworkowska, ; Ni et al, ; Niu et al, ; Oleszczuk et al, ; Simon et al, ; Sivanarayana et al, ; Speyer et al, ; Wang et al, ; Xue et al, ; Yang et al, ; Zheng et al, ).…”

Diagnostics of DNA fragmentation in human spermatozoa: Are sperm chromatin structure analysis and sperm chromatin dispersion tests (SCD‐HaloSpermG2 ® ) comparable?

“…When their semen was subjected to gradient selection, the proportion of damaged spermatozoa decreased and pregnancies‐to‐term were established. However, it should be considered that gradient selection, although selecting for DNA intactness using the SCD‐HaloSpermG2 ® assay, does not seem to statistically correlate with pregnancy‐to‐term after IVF (Comhaire et al, ; Zheng et al, ) or ICSI (Wang et al, ).…”

Efficient isolation of sperm with high DNA integrity and stable chromatin packaging by a combination of density-gradient centrifugation and magnetic-activated cell sorting

“…Recently, new methods to isolate sperm with high DNA integrity using density-gradient centrifugation (DGC) [2829] and magnetic-activated cell sorting (MACS) have been introduced [30]. DGC significantly reduced the sperm DFI in the semen of intracytoplasmic sperm injection patients [31] and in teratozoospermic patients [32]. MACS not only efficiently reduces the sperm DFI [33], but also effectively separates apoptotic from non-apoptotic spermatozoa [34].…”