Assessment of Detoxifying Markers for Florfenicol in Rainbow Trout Liver

Elia, Antonia Concetta; Pacini, Nicole; Fioravanti, Marialetizia; Dörr, Ambrosius Josef Martin; Zaccaroni, Annalisa; Parmeggiani, Alba Maria; Gustinelli, Andrea; Mordenti, Oliviero; Abete, Maria Cesarina; Prearo, Marino

doi:10.1080/08997659.2016.1206637

Cited by 19 publications

(8 citation statements)

References 54 publications

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“…It has been reported that amphenicols (Memik 1975) and oxytetracycline (Julinta et al 2019) are hepatotoxic so also our study with FFC. Also, the FFC can cause an increase in the weight of the liver (Elia et al 2016). Our results con rm the ndings of Er and Dik (2014), who observed a hike in AST levels upon FFC application in Oncorhynchus mykiss.…”

Section: Discussionsupporting

confidence: 91%

The effects of extended feeding of florfenicol coated medicated diets on the safety, serum biomarkers and blood cells morphology of Nile tilapia Oreochromis niloticus (L.)

Bardhan

Abraham

Singha

et al. 2022

Environ Sci Pollut Res

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Tilapia is one of the most consumed farmed sh, which requires the use of antibiotics in certain phases of its production. This study assessed the safety of 30 days of oral orfenicol (FFC)-dosing at 0-10 times the therapeutic dose (1X: 10 mg/kg biomass/day) in Oreochromis niloticus juveniles. Behavioural changes, feed consumption, mortality and biomass were evaluated. Besides, the levels of serum glucose, calcium, chloride, creatinine, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and blood cell morphology were determined at scheduled intervals. The 30 days of oral FFC-dosing caused 3.33% (1X) to 18.33% (10X) mortalities, reduced feed intake and biomass in a dosedependent manner. The sh fed the therapeutic dose recorded 1.25 folds increase in biomass; while the control group recorded 1.45 folds increase in 30 days. No signi cant erythrocyte morphological alterations were observed in the 1X group compared to the control. However, marked morphological alterations like tear-shaped, spindle-shaped and degenerative erythrocytes in higher dosing groups indicated FFC cytotoxicity. All the serum biomarkers of O. niloticus increased signi cantly on day 10 and day 30 FFC-dosing in a dose-dependent manner, except for calcium and chloride, which reduced signi cantly during the dosing period. Within 2 weeks of suspension of FFC-dosing, the serum biomarker levels became normal except for alkaline phosphatase and creatinine. The recovery of biomass, feed intake, serum biomarker levels and erythrocyte morphological changes suggested that the FFC induced changes are reversible. This study has, thus, proclaimed the safety of FFC at the therapeutic dose in O. niloticus.

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Section: Discussionsupporting

confidence: 91%

The effects of extended feeding of florfenicol coated medicated diets on the safety, serum biomarkers and blood cells morphology of Nile tilapia Oreochromis niloticus (L.)

Bardhan

Abraham

Singha

et al. 2022

Environ Sci Pollut Res

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Section: Discussionsupporting

confidence: 90%

Effects of Extended Feeding of Florfenicol Coated Medicated Diets on the Safety, Serum Biomarkers and Blood Cells Morphology of Nile Tilapia Oreochromis Niloticus (L.) Juveniles

Bardhan¹,

Abraham²,

Singha³

et al. 2021

Preprint

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Tilapia is one of the most consumed farmed fish, which requires the use of antibiotics in certain phases of its production. This study assessed the safety of 30 days of oral florfenicol (FFC)-dosing at 0-10 times the therapeutic dose (1X: 10 mg/kg biomass/day) in Oreochromis niloticus juveniles. Behavioural changes, feed consumption, mortality and biomass were evaluated. Besides, the levels of serum glucose, calcium, chloride, creatinine, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and blood cell morphology were determined at scheduled intervals. The 30 days of oral FFC-dosing caused 3.33% (1X) to 18.33% (10X) mortalities, reduced feed intake and biomass in a dose-dependent manner. The fish fed the therapeutic dose recorded 1.25 folds increase in biomass; while the control group recorded 1.45 folds increase in 30 days. No significant erythrocyte morphological alterations were observed in the 1X group compared to the control. However, marked morphological alterations like tear-shaped, spindle-shaped and degenerative erythrocytes in higher dosing groups indicated FFC cytotoxicity. All the serum biomarkers of O. niloticus increased significantly on day 10 and day 30 FFC-dosing in a dose-dependent manner, except for calcium and chloride, which reduced significantly during the dosing period. Within 2 weeks of suspension of FFC-dosing, the serum biomarker levels became normal except for alkaline phosphatase and creatinine. The recovery of biomass, feed intake, serum biomarker levels and erythrocyte morphological changes suggested that the FFC induced changes are reversible. This study has, thus, proclaimed the safety of FFC at the therapeutic dose in O. niloticus.

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“…Nevertheless, the reduced activity of this enzyme reported in our study indicates damage to the system in phase II of the FF detoxification process, making it difficult for fish to eliminate FF from their bodies. Elia et al (2016) evaluated the effect of FF on Rainbow Trout Oncorhynchus mykiss treated for 10 d with 7.5 and 15 mg/kg FF followed by a withdrawal period of 5 d,and detected oxidative damage during the antibiotic treatment as a consequence of the effect of FF toxicity and a rise in total glutathione and glutathione S-transferase levels, even after the withdrawal period.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of health and environmental risks for juvenile tilapia (Oreochromis niloticus) exposed to florfenicol

Shiroma

Soares

Cardoso

et al. 2020

Heliyon

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Intensive fish cultivation has a high incidence of infection, which is often controlled by administering antibiotics. Florfenicol (FF) is one of the two antimicrobial drugs permitted for aquaculture in Brazil. Due to their intensive use, potentially harmful effects on aquatic organisms are of great concern. In this sense, we investigated whether the presence of FF in cultivation water could change the health parameters of Nile tilapia. For this, we evaluated hemoglobin, hematocrit, mean corpuscular hemoglobin (MCHC) concentration, mean corpuscular volume (MCV), total plasma protein (TPP), number of circulating red blood cells and leukocytes, as lipid peroxidation levels, catalase activity and glutathione S-transferase activity of fish exposed to 11.72 mg L −1 of FF in water for 48 h. The fish were divided into two groups: Nile tilapia in water with FF or without FF (control). Exposure to FF in cultivation water for a short period didn't change the hematological variables analyzed, but caused changes in liver ROS (Reactive oxygen species) markers of the Nile tilapia, which was revealed by lipid peroxidation levels, catalase activity, and glutathione S-transferase. The 48h exposure period was enough to induce oxidative stress in hepatocytes, causing cellular oxidative damage. Therefore, the antibiotic florfenicol may cause toxicity to organisms and aquatic ecosystems, even at a sublethal concentrations near 1/100 LC50-48h for fish species.

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Assessment of Detoxifying Markers for Florfenicol in Rainbow Trout Liver

Cited by 19 publications

References 54 publications

The effects of extended feeding of florfenicol coated medicated diets on the safety, serum biomarkers and blood cells morphology of Nile tilapia Oreochromis niloticus (L.)

The effects of extended feeding of florfenicol coated medicated diets on the safety, serum biomarkers and blood cells morphology of Nile tilapia Oreochromis niloticus (L.)

Effects of Extended Feeding of Florfenicol Coated Medicated Diets on the Safety, Serum Biomarkers and Blood Cells Morphology of Nile Tilapia Oreochromis Niloticus (L.) Juveniles

Evaluation of health and environmental risks for juvenile tilapia (Oreochromis niloticus) exposed to florfenicol

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