Assessment of Disease Severity and  Role of Cytomegalo Virus Infection  in Patients with Ulcerative Colitis

Kalappurayil, Nobin Babu; Thomas, J; Mankuni, Baburajan; Thomas, Varghese

doi:10.7860/jcdr/2017/22816.9332

Cited by 1 publication

(1 citation statement)

References 26 publications

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“…Notably, CMV infections occur frequently in IBD patients, with high incidence of CMV reactivations particularly in steroid-refractory UC patients, and are associated with worse clinical outcomes in IBDs. 43 , 44 Intriguingly, we found that intestinal CCR5 + Th1/17 cells, the subset that reacted strongly with CMV, were selectively reduced in UC. These findings suggest that intestinal Th1/17 cells contain CMV infections, and may thus play an unexpected protective role in IBDs.…”

Section: Discussionmentioning

confidence: 68%

An Intestinal Th17 Subset is Associated with Inflammation in Crohn’s Disease and Activated by Adherent-invasive Escherichia coli

Paroni

Leccese

Ranzani

et al. 2023

Journal of Crohn's and Colitis

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IFNγ-producing ex-Th17-cells (“Th1/17”) were shown to play a key pathogenic role in experimental colitis and are abundant in the intestine. Here, we identified and characterized a novel, potentially colitogenic subset of Th17-cells in the intestine of patients with Crohn’s Disease (CD). Human Th17-cells expressing CCR5 (“pTh17”) co-expressed T-bet and RORC/γt and produced very high levels of IL-17, together with IFN-γ. They had a gene signature of Th17 effector cells and were distinct from established Th1/17-cells. pTh17-cells, but not Th1/17-cells, were associated with intestinal inflammation in CD, and decreased upon successful anti-TNF therapy with infliximab. Conventional CCR5(-)Th17-cells differentiated to pTh17 cells with IL-23 in vitro. Moreover, anti-IL-23 therapy with risankizumab strongly reduced pTh17-cells in the intestine. Importantly, intestinal pTh17-cells were selectively activated by adherent-invasive Escherichia coli (AIEC), but not by a commensal/probiotic E. coli strain. AIEC induced high levels of IL-23 and RANTES from DC. Intestinal CCR5 +Th1/17-cells responded instead to Cytomegalovirus and were reduced in UC, suggesting an unexpected protective role. In conclusion, we identified an IL-23-inducible subset of human intestinal Th17-cells. pTh17 cells produced high levels of pro-inflammatory cytokines, were selectively associated with intestinal inflammation in CD, and responded to CD-associated AIEC, suggesting a key colitogenic role.

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Section: Discussionmentioning

confidence: 68%