Banana micropropagation is commercially established by shoot tips obtained from intact plants on a shoot multiplication medium under optimal culture conditions. However, this is somewhat associated with tissue browning, which may be associated with reduced efficiency of the shoot multiplication process. It was found that changes in culture conditions such as increase or decrease of incubation temperature around 27 ºC, change in the texture of the media from the solid or liquid to semisolid state, addition of ascorbic acid, and substitution of optimal concentration of benzyl amino purine (5 mg/l BAP) with 2-isopentyl adenine (4 mg/l 2ip), could reduce tissue browning but decreased shoot multiplication and growth. The present results indicated that the chemical composition of the MS medium containing 5 mg/l BAP was ideal for banana shoot multiplication. Adding some natural ingredients rich in many important components (yeast extract, coconut endosperm milk, or moringa leaf extract) was unsuitable for in vitro multiplication of bananas. The observed exception was that the growth parameters of shoots formed on MS medium of 4 mg/l 2ip were better than those formed on MS of 5 mg/l BAP. In addition, shoot multiplication on 2-isopentenyl adenine (2ip) containing media showed root formation, adaptation, and transfer of plants to soil.