Assessment of DNA damage and lipid peroxidation in diabetic mice: Effects of propolis and epigallocatechin gallate (EGCG)

Oršolić, Nada; Sirovina, Damir; Gajski, Goran; Garaj‐Vrhovac, Vera; Jembrek, Maja Jazvinšćak; Kosalec, Ivan

doi:10.1016/j.mrgentox.2013.04.022

Cited by 63 publications

(61 citation statements)

References 50 publications

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“…This result is in agreement with previous investigations where animals suffering from insulin dependent diabetics (type 1) showed increased lipid peroxidation [21,33]. It was also reported that diabetic patients are more susceptible to membrane damage [32,34]. However, mixture of PUFAs from stem bark Alstonia boonei and antidiabetic drug (METAG) caused a significant reduction in MDA content of diabetic-induced rats.…”

Section: Discussionsupporting

confidence: 92%

“…This is in support of previous studies which reported phytochemicals as chemoprevention of diverse diseases and ailments [13]. Recent experimental studies have established the antioxidant and anti-diabetic properties of stem bark Alstonia boonei (SBAB) in experimental animal [26,32] and Escherichia coli [22] models. However, the underlying biochemical and especially molecular mechanisms of hepato-and testo-protection by SBEAB remain to be elucidated.…”

Section: Discussionsupporting

confidence: 88%

“…Posttreatment is most efficacious in inhibiting CAT activity in type 1 diabetic rat; followed by pre-and METAG-treatment respectively. Reduction on enzymatic antioxidant-CAT was complementary with the results of previous studies [32,36]. Also, protective effects of SBEAB on pre and post-treated group were due to free radical scavenging activity.…”

Section: Discussionsupporting

confidence: 83%

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PUFAs from Stem Bark of Alstonia boonei Synergistically Modulates Diabetic, Hepatic and Androgenic Damage by Low Expression of COX-2 and iNOS in Rats

Ajiboye¹,

Akintunde²,

So³

et al. 2017

J Mol Biomark Diagn

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PUFAs, from the stem bark extract of Alstonia boonei (SBEAB) was hypothesized to possess anti-inflammatory, antioxidant, anti-diabetic, pro-spermatogenic and hepatoprotective activities. The present study investigated the possible biochemical and molecular mechanisms underlying the hepatoprotective and testoprotective effects of SBEAB in diabetic rat. Biomarkers of hepatic and testicular damage, histological and immunohistochemical techniques were used. The expression of cyclooxygenase (COX-2) and inducible nitric oxide synthase (iNOS) were also estimated. SBEAB administered orally at dose of 100 mg/kg for 14 days significantly lowered the activities of serum transaminases and MDA levels induced by single intraperitoneal administration of streptozotoxin (STREP) (80 mg/kg) and preserved the integrity of both hepatocytes and spermatocytes. Also, SBEAB elevated the STREPinduced reduced activities of Δ5-17β-HSD and Δ5-17β-HSD with corresponding decrease in the activity of CAT. SBEAB inhibited the STREP induced expression of COX-2 and iNOS. The protective effect of SBEAB was compared to that of metaglomide (METAG), an established anti-diabetic drug. METAG treatment on hepatic damage was most efficacious in diabetic rats; followed by post and pre-treatment respectively while pre-and post-treatment were more efficacious on testicular damage than anti-diabetic drug. Furthermore, pre and post-treatment were more efficacious in preventing pro-inflammation and testicular cancer in diabetic rats than METAG-administration. We therefore concluded that the repression of genes encoding COX-2 and iNOS proteins by SBEAB validates the molecular basis of testicular protection and further suggests the links between the hepatocellular damage and male reproductive dysfunctions in diabetic individuals.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 83%

See 1 more Smart Citation

PUFAs from Stem Bark of Alstonia boonei Synergistically Modulates Diabetic, Hepatic and Androgenic Damage by Low Expression of COX-2 and iNOS in Rats

Ajiboye¹,

Akintunde²,

So³

et al. 2017

J Mol Biomark Diagn

View full text Add to dashboard Cite

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“…The elevated TBARS after 24 hours may be responsible for reduction in RNA and DNA levels. In a similar manner a significant increase in DNA strand breakdown observed after 24 hours in BPA exposed cells (Orsolic et al, 2013). Earlier studies on BPA metabolites have shown to bind to DNA in a cellular system (Chen et al, 2002).…”

Section: Discussionsupporting

confidence: 62%

Lipid Peroxidation Induced Nucleic Acid Damage in Liver and Brain of Bisphenol-a Treated Chick Embryos

2017

IJAPSA

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A study was conducted on effect of Bisphenol-A (BPA) toxicity on nucleic acid damage during embryonic development in chicks. Bisphenol-A dissolved in distilled water in three concentrations of 100µM, 250µM and 500µM injected into the aircell separately to 11 th and 14 th day old chick embryos. Embryos were sacrificed after 24 and 48 hours of BPA exposure to collect liver and brain tissues for estimation of Thiobarbituric acid reacting substances (TBARS) and nucleic acids (DNA & RNA). A dose dependent decrease in DNA levels was observed in all treatment groups after 24 hours in both 11 th and 14 th day BPA treated chick embryos compared to control. A significant dose dependent decrease in RNA levels was observed after BPA exposure after 24 hours in both 11 th and 14 th day BPA treated chick embryos compared to control.

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“…Due to the dominant role of antioxidant and antiglycation properties, EGCG are increasingly being investigated in diabetic nephropathy [31,32] . In a study performed on streptozotocin-induced diabetic rats fed with diet enriched with EGCG for 50 days, it has been shown that EGCG reduced the level of mRNAs for glyconeogenesis enzymes, suppressed hyperglycemia, proteinuria, and lipid peroxidation in diabetic nephropathy [33] .…”

Section: Diabetic Nephropathymentioning

confidence: 99%

The Green Tea Polyphenol(—)-epigallocatechin-3-gallate and its beneficial roles in chronic kidney disease

Bao

Peng

2016

Journal of Translational Internal Medicine

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Chronic kidney disease (CKD), a condition that affects around 10% of the population, has become a significant public health concern. Current therapeutic strategies to slow down the progression of CKD remain limited. Thus, it is urgent to develop new strategies to manage the patients with CKD. Work within the past decade has improved our understanding of the mechanisms contributing to CKD. In particular, oxidative stress as well as inflammation appears to play a pivotal role in CKD progression. (-)-Epigallocatechin-3-gallate (EGCG), the major catechin of green tea extract, is known as a powerful antioxidant and reactive oxygen species scavenger. Various studies have shown EGCG has a potential role in chronic kidney disease models. It is suggested that EGCG modulates cellular and molecular mechanisms via inflammation-related NF-κB and Nrf2 signaling pathway, as well as apoptosis-related ER stress pathway and mitochondrial pathway. Therefore, based on these studies, this review attempts to present a recent state of our knowledge and understanding of mechanisms of its role on the process of CKD, with the aim of providing some clues for the future optimization of EGCG in renal diseases.

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Assessment of DNA damage and lipid peroxidation in diabetic mice: Effects of propolis and epigallocatechin gallate (EGCG)

Cited by 63 publications

References 50 publications

PUFAs from Stem Bark of Alstonia boonei Synergistically Modulates Diabetic, Hepatic and Androgenic Damage by Low Expression of COX-2 and iNOS in Rats

PUFAs from Stem Bark of Alstonia boonei Synergistically Modulates Diabetic, Hepatic and Androgenic Damage by Low Expression of COX-2 and iNOS in Rats

Lipid Peroxidation Induced Nucleic Acid Damage in Liver and Brain of Bisphenol-a Treated Chick Embryos

The Green Tea Polyphenol(—)-epigallocatechin-3-gallate and its beneficial roles in chronic kidney disease

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