Assessment of DNA damage in car spray painters exposed to organic solvents by the high-throughput comet assay

Londoño-Velasco, Elizabeth; Martínez-Perafán, Fabián; Carvajal-Varona, Silvio; García-Vallejo, Felipe; Hoyos-Giraldo, Luz Stella

doi:10.3109/15376516.2016.1158892

Cited by 13 publications

(11 citation statements)

References 31 publications

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“…Only limited data were found in the literature that demonstrates health impacts experienced by auto body shop workers from VOCs and other hazardous chemicals used in auto body basecoat products. A 2016 study concluded that occupational exposure as a car spray painter was associated with DNA damage and the development of cancer (Londono-Velasco et al, 2016). Another study demonstrated that the use of appropriate personal protective equipment and good workplace hygiene were associated with reduced risk of symptoms of neurotoxicity in solvent-exposed vehicle spray painters (Keer et al, 2018).…”

Section: Social Impact Evaluationmentioning

confidence: 99%

Using an alternatives assessment framework to evaluate waterborne versus solventborne basecoats used in automotive refinishing

Brown

Fisk

Cohen

et al. 2021

Integr Envir Assess & Manag

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We evaluated two types of automotive basecoats used in automotive refinishing (i.e., in auto body shops): waterborne and solventborne. The primary tool we used for this evaluation was the Alternatives Assessment Guide (version 1.0) developed by the Interstate Chemicals Clearinghouse (IC2). The term “solventborne,” as used in this article, refers to products that contain relatively high concentrations of volatile organic compounds (referred to as “high‐volatile organic compound [VOC] basecoats” in the industry). These products are distinct from the “low‐VOC” solventborne products used widely in California and elsewhere. From a health and environmental hazard perspective, our evaluation found that waterborne basecoats contained fewer hazardous ingredients and at lower concentrations than their solventborne counterparts. Automotive painters who spray‐painted vehicles with waterborne products experienced significantly lower exposures to several harmful solvents. Waterborne products are readily available and offer advantages with regard to social impacts. Some of these advantages include lower worker and community exposure to VOCs, reduced VOC release and smog formation, and reduced potential for generation of and exposure to hazardous waste. Based on our assessment in auto body shops, we consider waterborne basecoats to be safer alternatives from both a human health and environmental perspective. Integr Environ Assess Manag 2022;18:1101–1113. Published 2021. This article is a U.S. Government work and is in the public domain in the USA.

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Section: Social Impact Evaluationmentioning

confidence: 99%

Using an alternatives assessment framework to evaluate waterborne versus solventborne basecoats used in automotive refinishing

Brown

Fisk

Cohen

et al. 2021

Integr Envir Assess & Manag

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“…It was reported that benzene within the range of 31.5 to 57.3 μg•m -3 can lead to health risks, such as leukemia (D. . Occupational exposure to organic solvent for sprayers has been associated with DNA damage and even caused cancer (Londoño-Velasco et al 2016). ARIs administrators generally provide personal protective equipment, such as respirator masks and allwrapped work clothes, with a certain prevention for sprayers.…”

Section: Introductionmentioning

confidence: 99%

High-solution emission patterns and health risks of volatile organic compounds from automobile repair industries in Beijing

Yao

et al. 2023

Preprint

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The increasing automobile repair industries (ARIs) with spray facilities have become an important volatile organic compounds (VOCs) pollution source in China. However, the VOCs health risk assessment for long-term exposure in ARIs have not been well characterized. In this study, though sampled VOCs from 51 typical ARIs in Beijing, the relationship between emission patterns, average daily exposure concentrations (EC) and health risks were comprehensively analyzed with the health assessment method. Results showed that concentrations of 117 VOCs from the samples ranged from 68.53 to 19863.32 µg·m− 3, aromatics were the main VOCs component in mix paint (MP) workshops and water-based paint spraying (WPS) workshops, accounting for 44.00% and 70.08%, respectively. And ARIs operator's daily VOCs inhalation EC was 11.24-1460.70 µg·m− 3. The method of inhalation exposure health risk assessment was firstly used to evaluate carcinogenicity and non-carcinogenicity risk for sprayers in ARIs. The cumulative lifetime carcinogenic risk (LCR) for 24 sampled VOCs were within acceptable ranges, while the mean hazard index (HI) with 44 sampled VOCs was over 1. Among them, the LCR and HI of VOCs in solvent-based spraying workshops were 8.72E-12 to 5.66E-7 and 0.13 to 22.37, and ethanol had a high carcinogenic risk. Of these, the mean HI associated with aromatics were 6.45 to 9.64. Our study will provide the important references for the standard of VOCs occupational exposure health limits in ARIs.

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“…Painting activity is giving work to more than 16,000 employees only in Italy. Several studies evaluated the potential adverse effects of exposure to toluene, xylenes, ethylbenzene, styrene and paints in painters, and they showed mainly oxidative and genotoxic effects [ 6 , 11 , 12 , 13 , 14 , 15 ]. In particular, DNA damage by comet assay, but not micronuclei induction was found by Moro et al [ 12 ] on lymphocytes of industrial painters exposed to low toluene levels.…”

Section: Introductionmentioning

confidence: 99%

Occupational Exposure in Industrial Painters: Sensitive and Noninvasive Biomarkers to Evaluate Early Cytotoxicity, Genotoxicity and Oxidative Stress

Cavallo

Ursini

Fresegna

et al. 2021

IJERPH

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This study aimed to identify sensitive and noninvasive biomarkers of early cyto-genotoxic, oxidative and inflammatory effects for exposure to volatile organic compounds (VOCs) in shipyard painters. On 17 (11 spray and 6 roller) painters (previously characterized for VOCs exposure to toluene, xylenes, ethylbenzene, ethyl acetate) and on 18 controls, we performed buccal micronucleus cytome (BMCyt) assay; Fpg-comet assay on lymphocytes; detection of urinary 8-oxoGua (8-oxo-7,8-dihydroguanine), 8-oxodGuo (8-oxo-7,8-dihydro-2′-deoxyguanosine) and 8-oxoGuo (8-oxo-7,8-dihydroguanosine), and cytokines release on serum. We found induction of cyto-genotoxicity by BMCyt assay and inflammatory effects (IL-6 and TNFα) in roller painters exposed to lower VOC concentrations than spray painters. In contrast, in both worker groups, we found direct and oxidative DNA damage by comet assay (with slightly higher oxidative DNA damage in roller) and significant increase of 8-oxoGuo and decrease of 8-oxodGuo and 8-oxoGua in respect to controls. The cyto-genotoxicity observed only on buccal cells of roller painters could be related to the task’s specificity and the different used protective equipment. Although limited by the small number of subjects, the study shows the usefulness of all the used biomarkers in the risk assessment of painters workers exposed to complex mixtures.

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Assessment of DNA damage in car spray painters exposed to organic solvents by the high-throughput comet assay

Cited by 13 publications

References 31 publications

Using an alternatives assessment framework to evaluate waterborne versus solventborne basecoats used in automotive refinishing

Using an alternatives assessment framework to evaluate waterborne versus solventborne basecoats used in automotive refinishing

High-solution emission patterns and health risks of volatile organic compounds from automobile repair industries in Beijing

Occupational Exposure in Industrial Painters: Sensitive and Noninvasive Biomarkers to Evaluate Early Cytotoxicity, Genotoxicity and Oxidative Stress

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