Assessment of DNA damage in children exposed to indoor tobacco smoke

Beyoğlu, Diren; Özkozaci, Tamay; Akıcı, Narin; Omurtag, Gülden Zehra; Akıcı, Ahmet; Ceran, Ömer; Şardaş, Semra

doi:10.1016/j.ijheh.2009.10.001

Cited by 10 publications

(5 citation statements)

References 22 publications

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“…A similar observation was described in another study, in workers from an elevator manufacturing factory in China, potentially exposed to benzene, were passive smoking at home, but not at the workplace, was significantly associated with DNA damage (37). In children, a significant increase in DNA damage has also been reported after exposure to ETS (41)(42)(43). No influence of age or gender in the DNA damage was observed in our study either in the total population or in the studied groups, although women presented a slightly lower level of DNA damage, as assessed by the percentage of DNA in tail.…”

Section: Discussionsupporting

confidence: 80%

“…A more evident genotoxic effect of ETS exposure appears to happen in children. A marginally significant increases of sister chromatid exchange (27), micronucleus (25,40) and DNA damage measured with the comet assay (41)(42)(43) was reported. In the occupational settings, the impact of ETS on the genotoxicity biomarkers remains to be clarified.…”

Section: Introductionmentioning

confidence: 99%

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Environmental Tobacco Smoke in Occupational Settings: Effect and Susceptibility Biomarkers in Workers From Lisbon Restaurants and Bars

Vital

Antunes

Louro

et al. 2021

Front. Public Health

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Environmental tobacco smoke (ETS) has been recognized as a major health hazard by environmental and public health authorities worldwide. In Portugal, smoke-free laws are in force for some years, banning smoking in most indoor public spaces. However, in hospitality venues such as restaurants and bars, owners can still choose between a total smoke-free policy or a partial smoking restriction with designated smoking areas, if adequate reinforced ventilation systems are implemented. Despite that, a previous study showed that workers remained continuously exposed to higher ETS pollution in Lisbon restaurants and bars where smoking was still allowed, comparatively to total smoke-free venues. This was assessed by measurements of indoor PM2.5 and urinary cotinine, a biomarkers of tobacco smoke exposure, demonstrating that partial smoking restrictions do not effectively protect workers from ETS. The aim of the present work was to characterize effect and susceptibility biomarkers in non-smokers from those hospitality venues occupationally exposed to ETS comparatively to non-exposed ones. A group of smokers was also included for comparison. The sister chromatid exchange (SCE), micronucleus (MN) and comet assays in whole peripheral blood lymphocytes (PBLs) and the micronucleus assay in exfoliated buccal cells, were used as biomarkers of genotoxicity. Furthermore, a comet assay after ex vivo challenge of leukocytes with an alkylating agent, ethyl methanesulfonate (EMS), was used to analyze the repair capacity of those cells. Genetic polymorphisms in genes associated with metabolism and DNA repair were also included. The results showed no clear association between occupational exposure to ETS and the induction of genotoxicity. Interestingly, the leukocytes from non-smoking ETS-exposed individuals displayed lower DNA damage levels in response to the ex vivo EMS challenge, in comparison to those from non-exposed workers, suggesting a possible adaptive response. The contribution of individual susceptibility to the effect biomarkers studied was unclear, deserving further investigation.

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Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Environmental Tobacco Smoke in Occupational Settings: Effect and Susceptibility Biomarkers in Workers From Lisbon Restaurants and Bars

Vital

Antunes

Louro

et al. 2021

Front. Public Health

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“…In the present study, children who exposed to environmental tobacco smoke (ETS) had a higher tail moment (3.01 ± 0.57) than those children who did not exposed to ETS (2.95 ± 0.70); however, no significant difference detected from the mean comparison. Studies by Zalata et al [ 70 ] and Beyoglu et al [ 71 ] demonstrated that exposure to ETS among children was positively proven can increase DNA damage. School-aged children are particularly more exposed to secondhand smoke compared with other age groups.…”

Section: Resultsmentioning

confidence: 99%

The Influence of Environmental Polycyclic Aromatic Hydrocarbons (PAHs) Exposure on DNA Damage among School Children in Urban Traffic Area, Malaysia

Hisamuddin

Jalaludin

Bakar

et al. 2022

IJERPH

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This study aimed to investigate the association between particulate PAHs exposure and DNA damage in Malaysian schoolchildren in heavy traffic (HT) and low traffic (LT) areas. PAH samples at eight schools were collected using a low volume sampler for 24 h and quantified using Gas Chromatography-Mass Spectrometry. Two hundred and twenty-eight buccal cells of children were assessed for DNA damage using Comet Assay. Monte-Carlo simulation was performed to determine incremental lifetime cancer risk (ILCR) and to check the uncertainty and sensitivity of the estimated risk. Total PAH concentrations in the schools in HT area were higher than LT area ranging from 4.4 to 5.76 ng m−3 and 1.36 to 3.79 ng m−3, respectively. The source diagnostic ratio showed that PAHs in the HT area is pyrogenic, mainly from diesel emission. The 95th percentile of the ILCR for children in HT and LT area were 2.80 × 10−7 and 1.43 × 10−7, respectively. The degree of DNA damage was significantly more severe in children in the HT group compared to LT group. This study shows that total indoor PAH exposure was the most significant factor that influenced the DNA damage among children. Further investigation of the relationship between PAH exposure and genomic integrity in children is required to shed additional light on potential health risks.

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“…Three studies addressed involuntary exposure to tobacco smoke, namely indoor tobacco smoke [ 122 ], second-hand cigarette smoking in children [ 155 ], and environmental tobacco smoking [ 161 ]. Two studies assessed exposure to ozone [ 147 , 153 ], one investigated the effects of formaldehyde under experimental conditions [ 164 ], and others looked at hair dye fumes [ 124 ] and coal mining residues [ 141 ].…”

Section: Resultsmentioning

confidence: 99%

The Comet Assay as a Tool in Human Biomonitoring Studies of Environmental and Occupational Exposure to Chemicals—A Systematic Scoping Review

Ladeira,

Møller,

Giovannelli

et al. 2024

Toxics

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Biomonitoring of human populations exposed to chemical substances that can act as potential mutagens or carcinogens, may enable the detection of damage and early disease prevention. In recent years, the comet assay has become an important tool for assessing DNA damage, both in environmental and occupational exposure contexts. To evidence the role of the comet assay in human biomonitoring, we have analysed original research studies of environmental or occupational exposure that used the comet assay in their assessments, following the PRISMA-ScR method (preferred reporting items for systematic reviews and meta-analyses extension for scoping reviews). Groups of chemicals were designated according to a broad classification, and the results obtained from over 300 original studies (n = 123 on air pollutants, n = 14 on anaesthetics, n = 18 on antineoplastic drugs, n = 57 on heavy metals, n = 59 on pesticides, and n = 49 on solvents) showed overall higher values of DNA strand breaks in the exposed subjects in comparison with the unexposed. In summary, our systematic scoping review strengthens the relevance of the use of the comet assay in assessing DNA damage in human biomonitoring studies.

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Assessment of DNA damage in children exposed to indoor tobacco smoke

Cited by 10 publications

References 22 publications

Environmental Tobacco Smoke in Occupational Settings: Effect and Susceptibility Biomarkers in Workers From Lisbon Restaurants and Bars

Environmental Tobacco Smoke in Occupational Settings: Effect and Susceptibility Biomarkers in Workers From Lisbon Restaurants and Bars

The Influence of Environmental Polycyclic Aromatic Hydrocarbons (PAHs) Exposure on DNA Damage among School Children in Urban Traffic Area, Malaysia

The Comet Assay as a Tool in Human Biomonitoring Studies of Environmental and Occupational Exposure to Chemicals—A Systematic Scoping Review

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