Assessment of DNA damage in Ardea cinerea and Ciconia ciconia: A 5-year study in Portuguese birds retrieved for rehabilitation

Santos, C.; Brandão, Ricardo; Monteiro, Marta S.; Bastos, Ana Catarina; Soares, Amadeu M.V.M.; Loureiro, Susana

doi:10.1016/j.ecoenv.2016.10.039

Cited by 17 publications

(15 citation statements)

References 30 publications

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“…(Shugart et al 2003; Baos et al 2006). More recently, the frequency of ENAs has been used as a marker to detect genotoxic damage in birds (De Mas et al 2015; Santos et al 2017b). Although its genotoxic effects in birds have been scarcely addressed in the literature, Hg has been suggested to have genotoxic effects (e.g., Fenstad et al 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have demonstrated the usefulness of assessing nuclear abnormalities as a complementary tool together with micronuclei frequency to diagnose the exposure to genotoxic agents (Braham et al 2017; López González et al 2017). However, ENA frequency seems to be a more sensitive marker of genotoxic damage than micronuclei alone (Santos et al 2017b). Moreover, the mechanisms underlying the development of nuclear abnormalities remain unclear (Oliveira et al 2010; Braham et al 2017), and it is not yet possible to discern whether these abnormalities are linked to loss of gene expression and, by extension, to fitness effects such as survival and reproduction.…”

Section: Discussionmentioning

confidence: 99%

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Mercury Uptake Affects the Development of Larus fuscus Chicks

Santos

Sotillo

Gupta

et al. 2020

Enviro Toxic and Chemistry

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Current emission and mobilization rates of mercury (Hg) in the environment pose extensive threats to both wildlife and human health. Assessing the exposure risk and effects of Hg contamination in model species such as seabirds is essential to understand Hg risks at the population and ecosystem levels. The lesser black-backed gull (Larus fuscus), a generalist seabird species, is an excellent model species because it forages in both marine and terrestrial habitats, which in turn differ in their Hg exposure risk. To identify possible deleterious effects of Hg exposure on developing L. fuscus chicks, a dietary experiment was carried out and chicks were provided a marine, terrestrial, or mixed diet. The effects of embryonic and dietary Hg exposure on chick body condition and physiological state were assessed at different developmental stages until fledging age (30 d). Overall physiological condition was lower in chicks fed a predominantly marine diet, which coincided with higher Hg loads in blood and primary feathers. However, no effect of dietary uptake of Hg was observed on body condition or in terms of genotoxic damage. Body condition and genotoxic damage correlated instead with Hg exposure during embryonic development, which seems to indicate that embryonic exposure to Hg may result in carry-over effects on later chick development.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Mercury Uptake Affects the Development of Larus fuscus Chicks

Santos

Sotillo

Gupta

et al. 2020

Enviro Toxic and Chemistry

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“…Therefore, the use of these two techniques together allows a broader view of the performance of genotoxic stressors (Martins and Costa, 2015). It is also important to highlight that the use of the sum of all abnormalities, or ENA, is recommended as it is noticeable, after a long time of empirical experience, that they are more efficient than micronuclei alone (Ayllon and Garcia-Vazquez, 2001;Van der Oost et al, 2003;Strunjak-Perovic et al, 2009;Santos et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Citogenotoxic response of juvenile cobia Rachycentron canadum (Linnaeus, 1766) reared in two different systems

et al. 2018

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Aquaculture production is continuously growing worldwide, and marine fish farming in Brazil is still in its infancy. Intensive farming conditions may cause physiological stress to the cultured organism, which can be evaluated by citogenotoxic biomarkers. The aim of this study was to assess the genotoxic effect of the rearing conditions in red blood cells of juvenile cobia Rachycentron canadum by using comet assay and micronucleus and other nuclear abnormalities assay. Juvenile cobia were reared for 13 weeks in indoor tank with open water circulation and in near shore cage. The comet assay and the nuclear abnormalities assay detected higher DNA damage and higher nuclear abnormalities frequency in erythrocytes of fish reared in the indoor tank. Results showed that two methods are complementary. Additionally, cobia were injected with ß-naphthoflavone (BNF) at concentrations of 2mgkg-1 and 10mgkg-1 in laboratory controlled conditions, and maintained for 7 days in separate tanks to better understand the response mechanisms of this species to a toxic substance. The comet assay did not detect any significant differences between BNF injected and control fish, whereas nuclear abnormalities assay showed significant differences between BNF injected and the control groups. The damages identified by the comet assay are repairable breaks in the DNA strands, whereas nuclear abnormalities may be permanent. Possibly the period of maintenance after injection was enough to clean BNF from the organisms and to repair the breaks in the DNA strands. As cobia seems to respond very well to genotoxic elements, comet assay and nuclear abnormalities assay would be useful tools to monitor farming conditions.

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“…These alterations represent impacts over DNA (Baesse et al 2015) and are indicatives of recent exposure to contaminants (Santos et al 2017 (Fritsch et al 2012;Quero et al 2016) are some of the factors responsible for metal contamination and alterations of the numbers of MN and NA in birds. Birds in higher trophic levels present higher concentrations of metals (Zolfaghari et al 2007;Lodenius and Solonen 2013;Abbasi et al 2015) due to the biomagni cation process (Burger and Gochfeld 1995;Tasneem et al 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Age is also an important factor for MN an NA. Different generations might present different levels of these alterations (Santos et al 2017;Tsarpali et al 2020). MN and NA increased with the reduction of the age classes in aquatic birds (Santos et al 2017), however, few studies tried to establish this relation.…”

Section: Introductionmentioning

confidence: 99%

Potential Use of Distinct Biomarkers (Trace Metals, Micronuclei and Nuclear Abnormalities) in a Heterogeneous Sample of Birds in Southern Brazil.

Tomazelli

Rodrigues

Franco

et al. 2021

Preprint

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The analysis of metal concentrations in feathers and the micronuclei (MN) and other nuclear abnormalities (NA) test in birds are tools used for evaluating the impacts that anthropogenic actions have been causing to the environment and organisms. Here we used these biomarkers to investigate the response of birds to disturbances observed in three areas with different environmental characteristics (natural, agricultural and urban) in southern Brazil. We obtained a diverse sample (108 individuals from 25 species and 17 families) without significant differences in metal concentrations, frequency of MN and AN between sampling points. The concentrations of zinc (Zn) and copper (Cu) were significantly different between trophic guilds (Zn: p = 0.0006, Cu: p = 0.04) and age classes (Zn: p = 0.01, Cu: p = 0.03). Omnivore birds contributed to the increase in the number of MN (∆AICc: 0.00; w = 0.40) and NA, which was also influenced by age classes and body condition index (BCI) (∆AICc: 0.00; w = 0.34; ∆AICc: 0.89; w = 0.22; ∆AICc: 1.15; w = 0.19; ∆AICc: 1.33; w = 0.18). We showed that in a diverse sample of birds, the variables analyzed affected each biomarker in distinct ways.

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Assessment of DNA damage in Ardea cinerea and Ciconia ciconia: A 5-year study in Portuguese birds retrieved for rehabilitation

Cited by 17 publications

References 30 publications

Mercury Uptake Affects the Development of Larus fuscus Chicks

Mercury Uptake Affects the Development of Larus fuscus Chicks

Citogenotoxic response of juvenile cobia Rachycentron canadum (Linnaeus, 1766) reared in two different systems

Potential Use of Distinct Biomarkers (Trace Metals, Micronuclei and Nuclear Abnormalities) in a Heterogeneous Sample of Birds in Southern Brazil.

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