Assessment of donor T‐cell function in cellular blood components by the CD69 induction assay: effects of storage, γ radiation, and photochemical treatment

Abstract

Flow cytometric measurement of CD69 induction can be adapted to provide quantitative assessment of T-cell function in blood components. Results obtained by the CD69 assay are in general agreement with those previously reported by use of proliferation methods; the assay may be useful for special applications in transfusion medicine.

Novel processes for inactivation of leukocytes to prevent transfusion-associated graft-versus-host disease

“…41,42 Using a mixed lymphocyte culture (MLC) assay, PEN 110-treated leukocytes were unable to proliferate; however, this assay system has a limited dynamic range (10 1 -10 2 ) compared to the larger dynamic range (10 5 -10 6 ) of the clonal T-cell expansion assay with LDA. 23 Leukocyte inactivation as measured by DNA modification Measurement of DNA modification by methods designed to inactivate T cells provides direct physical evidence of covalent binding of compounds or physical damage to leukocyte nucleic acid.…”

Quality control of riboflavin-treated platelet concentrates using Mirasol® PRT system: Polish experience

“…Clinical trials have proved the safety of Mirasol ® PRT system-treated blood components and lack of neoantigen formation. [8][9][10][11] Nowadays, the Mirasol ® PRT system is in routine use in many European countries. In Poland, the system has been used since 2009 for pathogen inactivation in plasma, but in some regional blood transfusion centers also for PCs.…”

Post-Transfusion Engraftment Syndromes: Microchimerism and TA-GVHD

“…150 The latter approach, which has been developed for pathogen inactivation in platelet and plasma units and is being evaluated in clinical trials, may be at least equally effective in preventing lymphocyte response to immunologic stimulation and proliferation. 150,151 …”