Assessment of Drug‐Induced Cardiotoxicity in Zebrafish

D’Amico, Louis J.; Seng, Wen Lin; Yang, Yi; Suter, Willi

doi:10.1002/9781118102138.ch4

Cited by 5 publications

(8 citation statements)

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“…The alterations observed in our study, namely pericardial oedema and heart beat reduction, are the same variations that were seen previously in other similar studies on zebrafish embryos and larvae affected by other toxic compounds, cardiotoxic also in humans and other mammals (D'Amico et al . ). These few reports together with our results suggest the need for further experiments to elucidate the mechanisms underlying this toxicity, using zebrafish as a model system, given that this fish now provides an excellent complement to the mouse to study the factors regulating both cardiac development and function (Chien , ; Christensen, Wang & Chien ).…”

Section: Discussionmentioning

confidence: 97%

“…The results from this study were in agreement with others published in zebrafish, in other vertebrate models and in humans (D'Amico et al . ). Previous work has already been undertaken (Anandhan et al .…”

Section: Introductionmentioning

confidence: 97%

“…Moreover, zebrafish is also useful in toxicological studies because small amounts of the compounds are required for the experiments on embryos and larvae of this little fish. Recently, a study was conducted (D'Amico et al 2012) to assess the effects on zebrafish of short-term exposure to 10 cardiotoxic drugs by LC 50 calculation, monitoring rhythmicity circulation and morphology. The results from this study were in agreement with others published in zebrafish, in other vertebrate models and in humans (D'Amico et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, a study was conducted (D'Amico et al 2012) to assess the effects on zebrafish of short-term exposure to 10 cardiotoxic drugs by LC 50 calculation, monitoring rhythmicity circulation and morphology. The results from this study were in agreement with others published in zebrafish, in other vertebrate models and in humans (D'Amico et al 2012). Previous work has already been undertaken (Anandhan et al 2013) to expose zebrafish embryos to different concentrations of Al for several hours.…”

Section: Introductionmentioning

confidence: 99%

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Aluminium chloride‐induced toxicity in zebrafish larvae

Antonio

Grimaldi

Ferrandino

2016

Journal of Fish Diseases

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Embryos at shield stage and larvae at protruding mouth stage were exposed to different concentrations of aluminium chloride (AlCl ) for 72 h with the purpose to analyse their phenotype and lethality. After 24, 48 and 72 h of treatment, higher toxicity of the metal was observed on larvae with minimal lethal concentration of 0.25, 0.20 and 0.08 mm, respectively, while for embryos the corresponding values were 40, 25 and 16 mm. We observed pericardial oedema and alteration of heart rate in 50% of larvae after 48 h of exposure to 100 μm. In larvae exposed to the same concentration, there was also a neurological injury at the level of glial cells, with the number of glial fibrillary acidic protein-positive cells being significantly reduced. This study confirms the toxic nature of this metal and shows that aluminium could also interestingly represent a cardiotoxin in addition to its neurotoxic ability.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Aluminium chloride‐induced toxicity in zebrafish larvae

Antonio

Grimaldi

Ferrandino

2016

Journal of Fish Diseases

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“…Furthermore, Louis J. D'Amico et al described methods that are used on the zebrafish for evaluating drug-induced cardiotoxicity [ 60 , 66 ]. Several drugs from different categories (vancer drugs, antiarrhythmic, anticonvulsant, and beta-blockers) are known to affect the heart function in patients were tested.…”

Section: Zebrafish Cardiotoxicity Studiesmentioning

confidence: 99%

Using Zebrafish for Investigating the Molecular Mechanisms of Drug-Induced Cardiotoxicity

Zakaria

Benslimane

Nasrallah

et al. 2018

BioMed Research International

104

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Over the last decade, the zebrafish (Danio rerio) has emerged as a model organism for cardiovascular research. Zebrafish have several advantages over mammalian models. For instance, the experimental cost of using zebrafish is comparatively low; the embryos are transparent, develop externally, and have high fecundity making them suitable for large-scale genetic screening. More recently, zebrafish embryos have been used for the screening of a variety of toxic agents, particularly for cardiotoxicity testing. Zebrafish has been shown to exhibit physiological responses that are similar to mammals after exposure to medicinal drugs including xenobiotics, hormones, cancer drugs, and also environmental pollutants, including pesticides and heavy metals. In this review, we provided a summary for recent studies that have used zebrafish to investigate the molecular mechanisms of drug-induced cardiotoxicity. More specifically, we focused on the techniques that were exploited by us and others for cardiovascular toxicity assessment and described several microscopic imaging and analysis protocols that are being used for the estimation of a variety of cardiac hemodynamic parameters.

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