Assessment of ecotoxicological effects of ciprofloxacin in Daphnia magna: life-history traits, biochemical and genotoxic effects

Nunes, Bruno; Leal, Cátia; Rodrigues, Sara; Antunes, Sara C.

doi:10.2166/wst.2018.255

Cited by 45 publications

(23 citation statements)

References 34 publications

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“…This result suggested that more evident genotoxic damage could be revealed by longer haemocyte exposure time, as recently observed in clam haemocytes exposed in vitro the new PFAS C6O4 (Fabrello et al, 2021). A significant increase in genetic damage index was detected in Daphnia magna starting from 0.013 mg/L of ciprofloxacin (Nunes et al, 2017).…”

Section: Discussionsupporting

confidence: 77%

Effects of Three Widely Used Antibiotics and Their Mixture on the Haemocytes of the Clam Ruditapes philippinarum

Nicolussi

Fabrello

Asnicar

et al. 2022

Front. Environ. Sci.

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Although the presence of pharmaceutical and personal care products in aquatic ecosystems is well documented, little information is available about their sublethal effects, on aquatic invertebrates. From an ecotoxicological point of view, the use of in vitro approaches has been recommended as a tool to assess adverse effects and to understand the mechanisms of action of chemicals at the cellular level. In the present in vitro study, the effects of Amoxicillin (AMX), Trimethoprim (TMP) and Ciprofloxacin (CIP) (1 µg/L, each) were tested alone and—for the first time—as a mixture (MIX) on haemocytes of the clam Ruditapes philippinarum. After the exposure, a battery of cellular parameters was evaluated, such as haemocyte viability, lysosomal membrane stability, superoxide anion production, acid phosphatase activity, the frequency of micronuclei and chromosomal aberrations. The results demonstrated that AMX, TMP, CIP and MIX affected lysosomal membrane stability, as well as superoxide anion and acid phosphatase production, and promoted chromosomal aberrations. This study highlighted that Manila clam haemocytes are a sensitive cell model to assess the effects of exposure to pharmaceutical products on non-target species. Our study demonstrated that the effects of pharmaceutical mixtures on marine species should be experimentally evaluated because they are not predictable from single exposures as the compounds can interact in different ways on the various biological endpoints considered.

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Section: Discussionsupporting

confidence: 77%

Effects of Three Widely Used Antibiotics and Their Mixture on the Haemocytes of the Clam Ruditapes philippinarum

Nicolussi

Fabrello

Asnicar

et al. 2022

Front. Environ. Sci.

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“…In addition, Dionísio et al (2020) highlighted that low levels of ciprofloxacin (0.0125–0.0625 mg/L) produced damage to the neuronal membrane due to an increase in thiobarbituric acid reactive substances (TBARS) in a chronic study (21 days) of D. magna . Similarly, Nunes et al (2018) report an increase in TBARS levels in adults exposed to 0.078 mg/L of ciprofloxacin (concentration, which is within the range 0.056–3.600 mg/L, Table 2, of this work) causing a decrease in the antioxidant defense of D. magna .…”

Section: Discussionsupporting

confidence: 74%

“…The toxicity of FQs on D. magna is due to the inhibition of the eukaryotic topoisomerase II enzyme that participates in the cellular replication of this cladocero (Hooper & Jacoby, 2016). In this regard, Nunes et al (2018) demonstrated that concentrations of 0.013 mg/L of ciprofloxacin induce genotoxic effects on D. magna .…”

Section: Discussionmentioning

confidence: 99%

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Toxicity of fluoroquinolones on the cladoceran Daphnia magna

Eluk

Nagel

Gagnetén

et al. 2021

Water Environment Research

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This study evaluates the acute and chronic toxicological effects of six fluoroquinolones on the mortality and growth of Daphnia magna. The NOECs calculated with the multivariate Probit regression model for the chronic study were 56 μg/L ciprofloxacin, 63 μg/L enrofloxacin, 78 μg/L levofloxacin, 85 μg/L marbofloxacin, 69 μg/L norfloxacin, and 141 μg/L ofloxacin. The risk quotients were determined using the measure environmental concentrations reported in water sources from different countries. The risks were low and moderate in water samples from rivers and lakes, although concentrations of ciprofloxacin, norfloxacin, and ofloxacin reported in some countries can cause toxicological damage to D. magna. In addition, urban wastewater and hospital wastewater samples constitute a threat to D. magna (high and moderate risks), requiring the treatment of these wastewater. Practitioner points The NOECs calculated with the multivariate Probit model for the six fluoroquinolonas are between 56 μg/L ciprofloxacin and 141 μg/L ofloxacin. The levels of ciprofloxacin, norfloxacin, and ofloxacin in urban wastewater and hospital wastewater produce moderate and high risks for D. magna. Water and river samples from some countries containing ciprofloxacin, norlfoxacin, and ofloxacin present high risks for D. magna.

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“…Furthermore, Daphnia is recognized as a sentinel species of freshwater ecosystems because its decline is an indicator of environmental impacts [3]. This characteristic associated with parthenogenic reproduction, short life cycle, high fecundity, and easy laboratory maintenance, makes them commonly used tool in toxicity assessments [11][12][13][14][15][16][17]. Normally, these organisms are maintained in laboratory conditions during several generations for use in biological research.…”

Section: Introductionmentioning

confidence: 99%

Does a Diet Rich in the Bacterium Rhodopirellula rubra Improve Daphnia magna Performance?

Marinho¹,

Jesus²,

Spencer³

et al. 2022

Front. Biosci. (Elite Ed)

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Background: In the wild various organisms contribute to daphnids diet. This study, intendeds to evaluate the potential of the concentration of Rhodopirellula rubra as a single or supplementary food source for Daphnia magna. Methods: Feeding assays were performed according to standard guidelines for chronic assays (21 days), and life-history parameters and several biomarkers (protein content, oxidative stress, energetic reserves and pigments) were measured. Five food regimens were conducted with 20 individual replicates (A -R. subcapitata; 0.2 -suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 -suspension of R. rubra at 0.4 AU; 0.2+A -suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga). Additionally, the effects of three diets (A, 0.2, and 0.2+A) on the longevity of D. magna were assessed. Results: The five diets showed a different C, N, and carotenoids composition, with an increase in the mixed diets. The results confirmed that the mixed diets improved D. magna life-history parameters. A decrease in glycogen, and the increase of haemoglobin, protein, and gluthione-S-transferase (GST) were observed. Furthermore, D. magna fed with bacterial single diets, presented worsen life history parameters and a decrease in the protein content. An induction of oxidative stress response (increased catalase and GST), and a significant decrease in lipid peroxidation and an accumulation of glycogen and carotenoids were observed. Overall, an increase in the amount of R. rubra provided to D. magna, from 0.2 AU to 0.4 AU, negatively impacted daphnid performance. No significant effects on Daphnia longevity (a 110-day assay) were observed among the three diets tested. However, a significant survival percentage and fertility (cumulative offspring is more than twice) was observed when D. magna was fed with the mixed diet. Conclusions: Results demonstrated that different diets provided a nutritional diversified food to the daphnids that induced differences in D. magna performance. The mixed diets proved to be beneficial (with increase in offspring) on D. magna performance, independently of the bacterial concentration tested. When in single diet, bacterial concentration is not nutritionally sufficient to raise D. magna even when in increased concentration.

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Assessment of ecotoxicological effects of ciprofloxacin in Daphnia magna: life-history traits, biochemical and genotoxic effects

Cited by 45 publications

References 34 publications

Effects of Three Widely Used Antibiotics and Their Mixture on the Haemocytes of the Clam Ruditapes philippinarum

Effects of Three Widely Used Antibiotics and Their Mixture on the Haemocytes of the Clam Ruditapes philippinarum

Toxicity of fluoroquinolones on the cladoceran Daphnia magna

Does a Diet Rich in the Bacterium Rhodopirellula rubra Improve Daphnia magna Performance?

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