Assessment of effectiveness of oral administration of collagen peptide on bone metabolism in growing and mature rats

Abstract

The purpose of this study was to examine the effectiveness of collagen peptide intake on bone metabolism in growing (G) and calcium-deficient mature (M) rats. As for the dosages used, they were amounts equal to the recommended supplements for humans (0.166 g/kg body weight (BW) per day: Coll-1G and Coll-1M groups), 10-fold higher (1.66 g/kg BW per day: Coll-10G and Coll-10M groups), and 100-fold higher (16.6 g/kg BW per day: Coll-100G group). In growing male rats, bone mineral density (BMD) of the femur in the Coll-100G group was significantly higher than that in the other groups after the 4-week experimental period. On the other hand, kidneys in the rats from the Coll-100G group exhibited hypertrophy. To examine the effects of collagen peptide on bone metabolism in a calcium-deficient status, mature female rats were fed a 0.01% Ca diet for 9 weeks and then fed a diet with 0.2% calcium with or without collagen peptide (control, Coll-1M, and Coll-10M groups) or a 0.5% calcium diet (normal Ca) for 8 weeks. BMD of the whole femur in the Coll-10M group was significantly higher than that in the control and Coll-1M groups, and the level was similar to that in the normal Ca group. BMD of the lumbar spine in the Coll-10M group was significantly higher than their baseline value, as well as being significantly higher than that in the control and Coll-1M groups. These results suggest that orally administered collagen peptide may provide beneficial effects on bone metabolism, especially in the calcium-deficient condition, without obvious undesirable effects.

Effects of Collagen Peptide Ingestion on Blood Lipids in Rats Fed a High-Lipid and High-Sucrose Diet

“…Oliveira et al (2001) reported that TG, but not TC, decreased significantly when mice were fed a 10% casein + 10% gelatin diet, compared with a 20% casein diet. Similar results were obtained when rats were fed a diet containing 0.166 g/kg body weight CP (Wu et al, 2004) or 0.17% CP (Saito et al, 2009). In contrast, blood TC levels were lower in rabbits fed a 12% casein + 8% gelatin diet than in rabbits fed TESTWako, respectively; Wako Pure Chemicals Co., Osaka, Japan).…”

“…In addition to their use as amino acid sources for protein synthesis in the body, ingested gelatin or CP affects various body functions. For example, gelatin ingestion results in increased bone mineral density and bone strength (Koyama et al, 2001;Wu et al, 2004). Meanwhile, CP supplementation increases the diameter and density of collagen fibrils, the major components of the dermis and tendons (Minaguchi et al, 2005;Matsuda et al, 2006), and the effects of CP are superior to those of lactalbumin for increasing bone mineral density (Koyama et al, 2001).…”

“…It has also been reported that ingestion of diets containing gelatin or CP affects the blood levels of triglycerides (TG) and/or total cholesterol (TC) in rats (Ratnayake et al, 1997;Wu et al, 2004;Saito et al, 2009), mice (Oliveira et al, 2001), and rabbits (Terpstra et al, 1983). TG and TC both decreased more markedly in rats fed a gelatincontaining diet than in rats fed a casein diet when a high protein content diet (300 g/kg diet) was used (Ratnayake et al, 1997).…”

Effects of Collagen-Derived Oligopeptide Prolylhydroxyproline on Differentiation of Mouse 3T3-L1 Preadipocytes

“…Wu et al (2004) reported that the ingestion of CP at a dose higher than 0.166 g/kg body weight for 4 weeks resulted in a significantly lower concentration of plasma triacylglycerides in rats. Saito et al (2009) reported that the ingestion of 0.4 g CP in combination with 0.5 mL soybean oil induced significantly lower serum triacylglyceride levels 2 h after ingestion.…”

“…Brittle nails are improved (Rosenburg et al, 1957), and hair diameter increases following the ingestion of gelatin (Scala et al, 1976). In addition, it was reported recently that serum triacylglycerides are decreased following ingestion of CP (Wu et al, 2004;Saito et al, 2009), suggesting the possibility that ingested CP modulates lipid metabolism by altering the function of the liver and/or adipocytes.…”