Assessment of Effects of Laser Light Combining Three Wavelengths (450, 520 and 640 nm) on Temperature Increase and Depth of Tissue Lesions in an Ex Vivo Study

Jurczyszyn, Kamil; Trzeciakowski, Witold; Woźniak, Zdzisław; Ziółkowski, Piotr; Trafalski, Mateusz

doi:10.3390/ma13235340

Cited by 4 publications

(3 citation statements)

References 35 publications

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“…The exposition of BJ-5ta cells to 520 nm irradiation, which represents green light spectra, decreased the viability of the cells only at the maximal fluence of 468.4 J/cm 2 . The decrease in viability was caused by necrosis ( Supplementary Data ), which was probably caused by the thermal effect, as shown by Jurczyszyn et al [ 31 ]. The green light exposition up to 468.4 J/cm 2 did not affect the cells in any other way.…”

Section: Discussionmentioning

confidence: 85%

Effects of 445 nm, 520 nm, and 638 nm Laser Irradiation on the Dermal Cells

Szymański

Ciepielak

Cios

et al. 2021

IJMS

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Background: The invention of non-ionizing emission devices revolutionized science, medicine, industry, and the military. Currently, different laser systems are commonly used, generating the potential threat of excessive radiation exposure, which can lead to adverse health effects. Skin is the organ most exposed to laser irradiation; therefore, this study aims to evaluate the effects of 445 nm, 520 nm, and 638 nm non-ionizing irradiation on keratinocytes and fibroblasts. Methods: Keratinocytes and fibroblasts were exposed to a different fluency of 445 nm, 520 nm, and 638 nm laser irradiation. In addition, viability, type of cell death, cell cycle distribution, and proliferation rates were investigated. Results: The 445 nm irradiation was cytotoxic to BJ-5ta (≥58.7 J/cm2) but not to Ker-CT cells. Exposure influenced the cell cycle distribution of Ker-CT (≥61.2 J/cm2) and BJ-5ta (≥27.6 J/cm2) cells, as well as the Bj-5ta proliferation rate (≥50.5 J/cm2). The 520 nm irradiation was cytotoxic to BJ-5ta (≥468.4 J/cm2) and Ker-CT (≥385.7 J/cm2) cells. Cell cycle distribution (≥27.6 J/cm2) of Ker-CT cells was also affected. The 638 nm irradiation was cytotoxic to BJ-5ta and Ker-CT cells (≥151.5 J/cm2). The proliferation rate and cell cycle distribution of BJ-5ta (≥192.9 J/cm2) and Ker-CT (13.8 and 41.3 J/cm2) cells were also affected. Conclusions: At high fluences, 455 nm, 520 nm, and 638 nm irradiation, representing blue, green, and red light spectra, are hazardous to keratinocytes and fibroblasts. However, laser irradiation may benefit the cells at low fluences by modulating the cell cycle and proliferation rate.

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Section: Discussionmentioning

confidence: 85%

Effects of 445 nm, 520 nm, and 638 nm Laser Irradiation on the Dermal Cells

Szymański

Ciepielak

Cios

et al. 2021

IJMS

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“…Synergistic effect of two or three wavelengths is very interesting. In our previously study we revealed synergistic effect of two wavelengths 450 and 520 nm on increase in tissue temperature [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Fractal Dimension and Texture Analysis of Lesion Autofluorescence in the Evaluation of Oral Lichen Planus Treatment Effectiveness

et al. 2021

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Background: Oral Lichen planus (OLP) is a chronic inflammatory disease. Topical steroids are used as the treatment of choice. The alternative is photodynamic therapy (PDT). The study aimed to fabricate optimal biodegradable matrices for methylene blue or triamcinolone acetonide because of a lack of currently commercially available carriers that could adhere to the mucous. Methods: The study was designed as a 12-week single-blind prospective randomized clinical trial with 30 patients, full contralateral split-mouth design. Matrices for steroid and photosensitizer and laser device were fabricated. Fractal and texture analysis of photographs, taken in 405, 450, 405 + 450 nm wavelength, of lesions was performed to increase the objectivity of the assessment of treatment. Results: We achieved two total responses for treatment in case of steroid therapy and one in the case of PDT. Partial response was noted in 17 lesions treated using local steroid therapy and 21 in the case of PDT. No statistically significant differences were found between the effectiveness of both used methods. Statistically significant differences in fractal dimension before and after treatment were observed only in the analysis of photographs taken in 405 + 450 nm wavelength. Conclusions: Photodynamic therapy and topical steroid therapy are effective methods for treating OLP. Using a carrier offers the possibility of a more predictable and effective method of drug delivery into the mucous membrane. Autofluorescence enables the detection of lesions especially at the early stage of their development.

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“…We also developed a similar laser at 520 nm (green emission), but with lower power (5 W). The setup shown in Figure 1 allows for the mixing of up to eight different wavelengths in the fiber and for simultaneous illumination with a few colors [ 8 ]. In the present study, we focused on clinical tests using the pure blue source.…”

Section: Methodsmentioning

confidence: 99%

Blue Laser (450 nm) in the Treatment of Port Wine Stains and Telangiectasia

Szymańczyk

Trzeciakowski

Ivonyak

et al. 2021

JCM

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This study aimed to test a blue light source for the treatment of port wine stains (PWS) and telangiectasia and to compare this with the application of green and yellow lasers based on data in the literature. A total of 22 patients with PWS were treated with radiation from a novel, high-power 450 nm blue laser that was created for this project. The group contained 15 patients with red PWS and 7 with pink PWS. The best results were achieved for red PWS, using 15–20 ms light pulses and 47 W power. For patients with pink malformations, the results were unsatisfactory. The group with telangiectasia consisted of six patients with facial lesions and three with leg lesions. The recovery was completed for all patients with facial telangiectasia, while the blue laser therapy was ineffective for patients with leg telangiectasia. This study shows that, in some cases, the use of a blue laser may be an alternative to the use of green and yellow lasers.

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Assessment of Effects of Laser Light Combining Three Wavelengths (450, 520 and 640 nm) on Temperature Increase and Depth of Tissue Lesions in an Ex Vivo Study

Cited by 4 publications

References 35 publications

Effects of 445 nm, 520 nm, and 638 nm Laser Irradiation on the Dermal Cells

Effects of 445 nm, 520 nm, and 638 nm Laser Irradiation on the Dermal Cells

Fractal Dimension and Texture Analysis of Lesion Autofluorescence in the Evaluation of Oral Lichen Planus Treatment Effectiveness

Blue Laser (450 nm) in the Treatment of Port Wine Stains and Telangiectasia

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