Urban agglomerations are actively developing concentrations of populated areas, which sometimes consist of ten's or even hundreds of populated areas, including rural settlements. Within the agglomeration, strong economic, social, labor and other connections are formed. At present, the trends of consolidation of cities into large agglomerated spaces are the subject of discussions at various forums, congresses and conferences on global scale. The processes of urbanization, expansion of settlements, population increase are always closely connected with the conservation and use of agricultural land, which makes the topic of research relevant. The article describes the practice of use of agricultural land of the largest agglomerations: the principles of using especially valuable land are presented, methods of preserving the territories used for agricultural purposes are analysed. Details of conservation and use of agricultural land are discussed in the article on the example of the Samara-Tolyatti agglomeration -the largest polycentric agglomeration of the Russian Federation. On the territory of this agglomeration, there is especially valuable agricultural land, significant area elements of the natural-ecological framework, which should be protected. Economic assessment of the use of agricultural land within the boundaries of agglomeration has been made and comparative analysis of use of agricultural land located within the boundaries of settlements has been presented. Also the features of the use of agricultural land depending on distance up to agglomeration centre have been discussed. The result of investigation is the development of conceptual proposals, on the basis of which it is possible to create an integrated methodology for evaluating alternatives of use of agricultural land for the purpose of rational land management in the agglomeration.