Assessment of Embryo-Induced Transcriptomic Changes in Hamster Uterus Using RNA-Seq

Huang, Mingyu; Zhang, Wenqian; Zhao, Miao; Zhu, Can; He, Jia-Peng; Liu, Ji‐Long

doi:10.1159/000489371

Cited by 7 publications

(3 citation statements)

References 59 publications

(57 reference statements)

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“…The top 20 down- and up-regulated DEGs in the CA endometrium have been identified in several studies. The SLC2A4, SLC24A2, SLC26A10, PLC1, PTGIS, MYLK, IL23A, WNT3 , and WNT11 genes in the maternal placenta (the chorion-attached endometrium) have been detected in the placenta-attached decidua of horses ( 27 ), rats ( 28 ), humans ( 22 ) and hamsters ( 29 ). The solute carrier (SLC) superfamily is the largest group of membrane transporters, comprising 65 families (SLC1-65) with more than 400 identified genes, and SLC2A4 is in the glucose/glucosamine transporter family ( 30 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Comparison of Chorion-Attached and Non-chorion-attached Endometrium in Mid-gestation of Rabbit

Mei

Ren

et al. 2022

Front. Vet. Sci.

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BackgroundThe chorion from the placenta is directly attached to the endometrium (CA) after embryo implantation while some parts of the endometrium are not chorion-attached (NCA). The differences in gene expression between the CA and NCA endometrium mid-gestation are unknown. Our objective was to compare the gene expression profiles of the CA and NCA endometrium of rabbit, to identify the differentially expressed genes (DEGs), and correlate the differences with the physiological state of the endometrium at mid-gestation of rabbit.MethodsWe used transcriptome sequencing to reveal the differences in gene expression between CA and NCA endometrium (n = 3), and then determined the concentration of inflammatory cytokines in CA and NCA tissue and serum by ELISA.ResultsSix Hundred and Forty-Six DEGs were identified between the CA and NCA endometrium [p < 0.05, |log2 (fold change) |≥ 2], The expression levels of 590 DEGs were higher in the NCA endometrium than in the CA endometrium, while the expression level of only 56 DEGs were higher in CA than in NCA. The DEGs were enriched in gene ontology (GO) terms and pathways related to immune regulation and cellular adhesions. Six hub-genes related to inflammatory mediator regulation of transient receptor potential (TRP) channels and chemokine signaling pathways had a lower expression level in the CA endometrium compared to the NCA endometrium, and the expression levels of genes related to focal adhesion and extracellular matrix (ECM)-receptors were significantly higher in NCA endometrium than in CA endometrium. The level of pro-inflammatory cytokines accumulated in the CA endometrium, and high abundance of integrin-β and THBS1 were localized in the luminal epithelium of the NCA endometrium, but not in the CA endometrium.ConclusionsOur study reveals differences in gene expression between the CA and NCA endometrium at mid-gestation of rabbit, and suggests implications for endometrial physiological function. The CA endometrium showed relative low-level gene expression compared to the NCA endometrium, while the NCA endometrium performed physiological functions related to focal adhesion and ECM-receptor interaction.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Comparison of Chorion-Attached and Non-chorion-attached Endometrium in Mid-gestation of Rabbit

Mei

Ren

et al. 2022

Front. Vet. Sci.

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“…High-throughput sequencing was run on an Illumina HiSeq 2500 system. Raw RNA-seq data were processed with an in-house computational pipeline as described previously [ 13 ]. Briefly, clean reads were mapped to the mouse genome (UCSC mm9) with the TopHat software v2.0.4 [ 14 ] and then assembled at the gene level with the Cufflinks software v2.2.1 [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

Comparative Analysis of Mouse Decidualization Models at the Molecular Level

Wang

Zhao

Zhang

et al. 2020

Genes

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The mouse is widely used to study decidualization and there are three well-established mouse models of decidualization, namely natural pregnancy decidualization (NPD), artificial decidualization (AD), and in vitro decidualization (IVD). However, the extent of similarity and difference between these models at the molecular level remains largely unknown. Here, we performed a comparative analysis using the RNA-seq approach. In the NPD model, which is thought to be the golden standard of mouse decidualization, we found a total of 5277 differentially expressed genes, with 3158 genes being up-regulated and 2119 genes being down-regulated. A total of 4294 differentially expressed genes were identified in the AD model: 1127 up-regulated genes and 3167 down-regulated genes. In comparison to NPD, 1977 genes were consistently expressed, whereas only 217 genes were inconsistently expressed, indicating that AD is a reliable model for mouse decidualization. In the IVD model, RNA-seq analysis revealed that 513 genes were up-regulated and 988 genes were down-regulated. Compared to NPD, 310 genes were consistently expressed, whereas 456 genes were inconsistently expressed. Moreover, although the decidualization marker Prl8a2 (prolactin family 8 subfamily a member 2) was up-regulated, the widely-used marker Alpl (alkaline phosphatase liver/bone/kidney) was down-regulated in the IVD model. Therefore, we suggest that the IVD model should be optimized to mimic NPD at the transcriptomic level. Our study contributes to an increase in the knowledge about mouse models of decidualization.

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“…High-throughput sequencing was performed using the Illumina HiSeq 2500 system. After sequencing, raw data were processed by a computational pipeline as described previously (Huang et al, 2018). Raw data were first aligned to mouse genome (UCSC mm9) using TopHat v2.0.4 with default options (Trapnell et al, 2009) and then assembled using Cufflinks v2.2.1 (Trapnell et al, 2010).…”

Section: Methodsmentioning

confidence: 99%

Identification of Gene Expression Changes Associated With Uterine Receptivity in Mice

He¹,

Zhao²,

Zhang³

et al. 2019

Front. Physiol.

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The mouse is a widely used animal model for studying human reproduction. Although global gene expression changes associated with human uterine receptivity have been determined by independent groups, the same studies in the mouse are scarce. The extent of similarities/differences between mice and humans on uterine receptivity at the molecular level remains to be determined. In the present study, we analyzed global gene expression changes in receptive uterus on day 4 of pregnancy compared to non-receptive uterus on day 3 of pregnancy in mice. A total of 541 differentially expressed genes were identified, of which 316 genes were up-regulated and 225 genes were down-regulated in receptive uterus compared to non-receptive uterus. Gene ontology and gene network analysis highlighted the activation of inflammatory response in the receptive uterus. By analyzing the promoter sequences of differentially expressed genes, we identified 12 causal transcription factors. Through connectivity map (CMap) analysis, we revealed several compounds with potential anti-receptivity activity. Finally, we performed a cross-species comparison against human uterine receptivity from a published dataset. Our study provides a valuable resource for understanding the molecular mechanism underlying uterine receptivity in mice.

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Assessment of Embryo-Induced Transcriptomic Changes in Hamster Uterus Using RNA-Seq

Cited by 7 publications

References 59 publications

Transcriptome Comparison of Chorion-Attached and Non-chorion-attached Endometrium in Mid-gestation of Rabbit

Transcriptome Comparison of Chorion-Attached and Non-chorion-attached Endometrium in Mid-gestation of Rabbit

Comparative Analysis of Mouse Decidualization Models at the Molecular Level

Identification of Gene Expression Changes Associated With Uterine Receptivity in Mice

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