Assessment of environmental pollution at Balearic Islands applying oxidative stress biomarkers in the mussel Mytilus galloprovincialis

Box, Antonio; Sureda, Antoni; Galgani, François; Pons, Antoni; Deudero, Salud

doi:10.1016/j.cbpc.2007.06.006

Cited by 87 publications

(54 citation statements)

References 45 publications

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“…This may be a reflection either of a decrease in contaminants inducing LPO or an adaptive antioxidant response over time to counteract ROS generation leading to a lower lipid peroxidation. M. galloprovincialis from low to high impacted sites on the NW coast of Portugal (Lima et al 2007) and Balearic Islands in Spain (Box et al 2007) showed slightly higher LPO values (9-12 and 7.7-12.1 μmol g -1 Prot, respectively) compared to the ones obtained in this study, which are indicative of the low or moderate levels of contamination found in mussels from the Ria Formosa Lagoon (Cravo et al 2009). As for clams, LPO values were similar to those found for the same species in the same lagoon in previous years (0.4-0.6 μmol g -1 Prot, Géret and Bebianno 2004;Bebianno and Barreira, 2009) and relatively lower than in Thau lagoon in France (0.643 μmol g -1 Prot, Bebianno et al 2004).…”

Section: Mytilus Galloprovincialiscontrasting

confidence: 66%

Interspecific variability of endocrine disruption and oxidative stress in two bivalve species from the Ria Formosa Lagoon (south coast of Portugal)

Pereira¹,

Gomes²,

Cardoso³

et al. 2013

Sci. Mar.

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SUMMARY: Estuaries are highly productive ecosystems that have been increasingly affected by anthropogenic activities. In this study, the presence of endocrine disrupting compounds (EDCs) and their relation to oxidative stress were assessed in two bivalve species (mussels Mytilus galloprovincialis and clams Ruditapes decussatus) from the Ria Formosa Lagoon (south Portugal). We applied the alkali-labile phosphate (ALP) assay to determine vitellogenin-like protein levels, and the lipid peroxidation (LPO) method to assess oxidative damage. Mussels and clams exhibited the same pattern of ALP concentrations, with no differences between genders and higher levels in winter. R. decussatus showed higher ALP values than M. galloprovincialis, possibly associated with a stronger responsiveness of clams to EDCs or due to the influence of the reproductive cycle. Mussels exhibited a more evident seasonal effect for LPO, indicating that these organisms are more influenced by seasonal fluctuations in the water column compared to the sediment-associated clams. Both species appear to be under the influence of EDC exposure (which could induce Vg-like protein synthesis) and oxidative stressors (leading to lipid peroxidation) in the Ria Formosa Lagoon, particularly in winter, reflecting the presence of different contaminants in Ria Formosa and environmental changes, as well as complex interactions between them.Keywords: Ria Formosa Lagoon, Mytilus galloprovincialis, Ruditapes decussatus, endocrine disruption, vitellogenin-like proteins, lipid peroxidation. RESUMEN: Variabilidad interespecífica de disruptores endocrinos y estrés oxidatiVo en dos especies de biValVos de la ría formosa (costa sur de portugal). -Los estuarios son ecosistemas altamente productivos afectados por actividades antropogénicas. En este trabajo, la presencia de compuestos disruptores endocrinos (EDCs) y su relación con estrés oxidativo se ha evaluado en los bivalvos Mytilus galloprovincialis y Ruditapes decussatus en la laguna Ría Formosa (Portugal). Se utilizó el ensayo de fosfatos lábil alcalinos (ALP) para determinar los niveles de proteína tipo-vitelogenina y el método de peroxidación lipídica (LPO) para evaluar los daños oxidativos. Ambas especies exhibieron el mismo patrón de concentración de ALP: no hubo diferencias entre sexos pero si niveles más altos en invierno. R. decussatus presentó valores mayores de ALP que M. galloprovincialis posiblemente asociados a una capacidad de respuesta más elevada a los EDCs o al estado del ciclo reproductor. Los mejillones, al no estar asociados al sedimento como las almejas, mostraron variaciones estacionales en las concentraciones de LPO, indicando mayor sensibilidad a fluctuaciones ambientales en la columna de agua. Ambas especies parecen estar influenciadas (principalmente en invierno) por EDCs (posiblemente induciendo la síntesis de proteína tipo-vitelogenina) y factores de estrés oxidativo (activando la peroxidación lipídica); lo que refleja la presencia de diferentes contaminantes en la Ría Formosa y los cambios a...

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Section: Mytilus Galloprovincialiscontrasting

confidence: 66%

Interspecific variability of endocrine disruption and oxidative stress in two bivalve species from the Ria Formosa Lagoon (south coast of Portugal)

Pereira¹,

Gomes²,

Cardoso³

et al. 2013

Sci. Mar.

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“…Lipid peroxidation products, CoD and TBARS, are widely used as markers of oxidative stress in aquatic organisms caused by pollution [9,33]. In 1999, the content of these products in mussels from Site 1 was 2 times higher compared to that in mussels from Site 2 [10]; while in 2011, only the TBARS content was 85% higher [12].…”

Section: Discussionmentioning

confidence: 99%

“…Induction of antioxidant enzymes is known to be an important compensatory response to stress caused by environmental pollution [8,9]. Therefore it can be assumed that the increased SOD activity prevents lipid peroxidation in mussels from Site 1 and is a consequence of the mussels' adaptation to chronic pollution.…”

Section: Issn: 2471-4879mentioning

confidence: 99%

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Effect of Marine Environment Remediation on Oxidative Stress Indicators and Metals in the Digestive Gland of the Mussel Crenomytilus grayanus

2016

J Environ Stud

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The marine environment quality in Gornostai Bay of the Peter the Great Bay, East Sea/Sea of Japan (Russia's Primorski Krai) was assessed based on oxidative stress biomarkers and the content of heavy metals in the digestive gland of the mussel Crenomytilus grayanus. Mussels were collected in Gornostai Bay in 2013, three years after the remediation of a domestic solid waste dump site located on the shore of the bay and in a relatively clean area at Reineke Island (Peter the Great Bay). The results were compared with the data from the studies performed on C. grayanus from the two locations in 1999 and 2011. It is concluded that the marine environment quality in Gornostai Bay is progressively (gradually) improving three years after the remediation of the domestic solid waste dump site compared to 1999 and 2011. IntroductionAerobic species have evolved the capacity to use oxygen for the efficient release of energy. However, in vitro studies suggest that 1-3% of the oxygen molecules used are converted to reactive oxygen species (ROS), including both radical and non-radical species . Therefore, the ROS are continually produced as toxic bi-products of normal metabolism from various endogenous processes [1]. Key biological molecules, notably DNA, proteins, lipids, can all be adversely affected by ROS [2,3]. Furthermore, the reaction of ROS with these macromolecules generates additional ROS, setting in train a cascade of damage if left unchecked [4]. An antioxidant system (represented by low molecular weight free radical scavengers and specific antioxidant enzymes) is present in the body of all aerobes in order to retard the ROS generation processes and to protect the cellular components from oxidation and to repair the oxidized biomolecules [5].The accumulation of heavy metals, petroleum hydrocarbons and polychlorinated phenols in the tissues promotes the formation of ROS [1,6,7]. Molecular biomarkers of oxidative stress (oxidative degradation products of biological molecules and the levels of antioxidants) have been widely used in recent decades to identify the toxic effect of pollutants [8,9].The aim of this study was to use biochemical indicators to assess changes in the marine environment quality after remediation of the anthropogenically polluted coastal zone. For this purpose, the content of heavy metals (Fe, Zn, Cu, Cd and Pb) and indicators of oxidative stress (activity of antioxidant enzymes and levels of reduced glutathione and lipid peroxidation products -diene conjugates, TBAreactive products) as well as total lipid content were determined in the digestive gland of Crenomytilus grayanus (Dunker 1853). Furthermore, studies focused both on the dynamics of release of heavy metals from digestive gland tissue of mussel and the dynamics of changes in biochemical indicators after remediation of the anthropogenically polluted coastal zone are of significant interest.Therefore, a change in the marine environment quality in the Gornostai Bay in 2013 was evaluated based on the changes in contents of metals and b...

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“…According to Box et al (2007), CAT and GPx play a complementary role in the elimination of H 2 O 2 . CAT is active at rather high H 2 O 2 concentrations, while playing a minor role in the catabolism of H 2 O 2 at low production.…”

Section: Discussionmentioning

confidence: 99%

Environmental impact on the antioxidant responses inCorbicula fluminea(Bivalvia: Veneroida: Corbiculidae) from the Danube River

Vranković

2015

Italian Journal of Zoology

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The present study aims to assess the biological effects of different environmental pollution on the Asian clam Corbicula fluminea. Biomarkers indicative of oxidative stress (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase, GPx; glutathione reductase, GR; and the phase II biotransformation enzyme glutathione-S-transferase, GST) were measured in clams collected within the Serbian sector of the Danube River Basin at three locations: Višnjica, Zemun and Bela stena. Clams from Višnjica exhibited significantly higher SOD, CAT and GST activities than clams from the other two locations (p < 0.05), whereas GPx and GR activities do not have such a marked pattern. An alteration in the activity of SOD, CAT and GST reflects the presence of certain pro-oxidative chemicals that can lead to oxidative stress in clams at the Višnjica site. In conclusion, the noted antioxidant disparities among clam populations from different locations reflected physiological sensitivity of C. fluminea to environmental change, supporting its use as a sentinel organism in biomonitoring investigations.

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Assessment of environmental pollution at Balearic Islands applying oxidative stress biomarkers in the mussel Mytilus galloprovincialis

Cited by 87 publications

References 45 publications

Interspecific variability of endocrine disruption and oxidative stress in two bivalve species from the Ria Formosa Lagoon (south coast of Portugal)

Interspecific variability of endocrine disruption and oxidative stress in two bivalve species from the Ria Formosa Lagoon (south coast of Portugal)

Effect of Marine Environment Remediation on Oxidative Stress Indicators and Metals in the Digestive Gland of the Mussel Crenomytilus grayanus

Environmental impact on the antioxidant responses inCorbicula fluminea(Bivalvia: Veneroida: Corbiculidae) from the Danube River

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