Assessment of environmental stress by the micronucleus test and the Comet assay on the genome of teleost populations from two natural environments

Russo, Consiglia; Rocco, Lucia; Morescalchi, M. A.; Stingo, Vincenzo

doi:10.1016/s0147-6513(03)00027-7

Cited by 137 publications

(72 citation statements)

References 48 publications

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“…The DNA damage detected in this study could have originated from DNA single strand breaks, DNA double strand breaks, DNA adducts formation and DNA-DNA and DNA-protein cross links (Mitchelmore and Chipman, 1998), resulting from the interaction of pesticides or their metabolites with DNA (Fairbairn et al, 1995). The DNA damage can be correlated with the clastogenecity of the mutagen because an increase in both micronuclei and DNA migration due to environmental mutagen has been reported in fish (Russo et al, 2004). There are few previous studies which showed dimethoate to be toxic particularly in fish (Dogan et al, 2011).…”

Section: Discussionmentioning

confidence: 64%

Evaluation of genotoxic and oxidative stress response to dimethoate in freshwater fish Channa punctatus (Bloch)

Ali

Kumar

et al. 2014

Chemical Speciation & Bioavailability

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Dimethoate is one of the organophosphate insecticides widely used in agriculture throughout the world and is an inhibitor of cholinesterase in animals. The objective of the present study was to detect oxidative stress and DNA damage induced by dimethoate in the freshwater fish Channa punctatus (C. punctatus). The LC 50 -96 h value of technical grade dimethoate was estimated at 19.10 µg L -1 in a semi-static system and, on the basis of the LC 50 value, three concentrations were determined. The fish were exposed to these concentrations of dimethoate for 96 h and samplings were done at 24 and 96 h for assessment of oxidative stress and DNA damage. After exposure to dimethoate, the level of superoxide dismutase declined while lipid peroxidation, glutathione, induction of micronucleus and DNA damage were increased in C. punctatus as the concentration and exposure time increased. Thus our results suggest that dimethoate induces genotoxic effects which invariably accompanied and correlated with oxidative stress.

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Section: Discussionmentioning

confidence: 64%

Evaluation of genotoxic and oxidative stress response to dimethoate in freshwater fish Channa punctatus (Bloch)

Ali

Kumar

et al. 2014

Chemical Speciation & Bioavailability

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“…Eritrócitos de sangue periférico são muito comumente usados para a aplicação do Ensaio do Cometa em conjunto com o Teste do MN, utilizando peixes como bioindicadores para avaliação ambiental de contaminação genotoxicológica (Belpaeme, et al 1996;Da Silva et al 2002;Russo et al 2004;Buschini, et al;.…”

Section: Discussionunclassified

Avaliação da mutagênese e genotoxicidade em Eigenmannia virescens (Teleostei: Gymnotiformes) expostos ao benzeno

2006

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RESUMOOs efeitos de substâncias genotóxicas sobre o genoma de peixes tem sido objeto de muitos estudos, sobretudo daqueles que buscam estabelecer a resposta dos genes aos estímulos ambientais. O presente trabalho teve como objetivo realizar um estudo sobre mutagenicidade e genotoxicidade em peixes elétricos da espécie Eingenmannia virescens, pela exposição ao benzeno (50ppm), utilizando as técnicas da Freqüência de Micronúcleos (MNs) e o Ensaio do Cometa. Foram coletadas amostras do sangue de dez peixes em diferentes tempos de exposição: T 0 , 24h, 48h, 72h, 96h e 360h (15 dias). Para a análise das lâminas no Teste do MN, foram contadas 1.000 células e estipulada a freqüência de ocorrência de MNs. Para análise do Ensaio do Cometa a contagem foi feita estipulando quatro classes de danos: I -II -III -IV, e para a análise estatística foram atribuídos valores numéricos (ranques) de 0 a 3, respectivamente, verificando diferenças significativas para a soma dos ranques em todos os tempos de exposição em relação ao T 0 . No Teste do Micronúcleo não foi possível detectar efeitos mutagênicos significativos nos eritrócitos analisados. No entanto, para o Ensaio do Cometa os resultados sugerem ação genotóxica do benzeno, devido a um aumento gradual no número de células com maiores classes de danos de acordo com maior tempo de exposição, indicando um efeito tempo-dependente. Estes resultados sugerem maior sensibilidade do Ensaio do Cometa que o Teste do MN. PALAVRAS-CHAVETeste do Micronúcleo; Ensaio do Cometa; peixes elétricos; eritrócitos Eingenmannia virescens, under to benzene exposure (50ppm), utilizing Micronuclei Test (MNs) and Comet Assay techniques. Ten fish blood samples were collected in different times of exposure : T 0 , 24h, 48h, 72h, 96h and 360h (15 days Avaliation of mutagenicity and gentotoxicity in Eigenmannia virescens (Teleostei: Gymnotiformes) exposed to benzene ABSTRACT The effects of genotoxic substances on fishes genome have been object of many studies, especially those which attempt to establish the responses of genes to environmental stimulus. The objective of this study was to evaluate the mutagenicity and genotoxicity in electric fishes of species

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“…Therefore the use of sensitive biomarkers in sentinel species has become a major issue in environmental genotoxicity monitoring (6,7).…”

mentioning

confidence: 99%

“…Fish are often organisms of choice in environmental biomonitoring because of their role in biotic communities and because of their sensitivity to mutagens and environmental pollutants, even at low concentrations (6,8,9). Several studies have investigated the genotoxic effects of polluted aquatic environments on fish species, either through the sampling of native populations (6,(9)(10)(11)(12)(13) or by cage exposure in situ (14)(15)(16).…”

mentioning

confidence: 99%

“…Several studies have investigated the genotoxic effects of polluted aquatic environments on fish species, either through the sampling of native populations (6,(9)(10)(11)(12)(13) or by cage exposure in situ (14)(15)(16). When assessing the impact of pollution in aquatic environments using native fi sh populations, research may be compromised by migration of fi sh for feeding and breeding, availability of certain species at a particular site of interest, or diffi culty to obtain enough specimens for desired analyses.…”

mentioning

confidence: 99%

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Cage Exposure of European Sea Bass (Dicentrarchus Labrax) for in Situ Assessment of Pollution-Related Genotoxicity

Šrut

Štambuk

Pavlica

et al. 2010

Archives of Industrial Hygiene and Toxicology

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Genotoxic effects are often the earliest signs of pollution-related environmental disturbance. In this study, we used the comet assay and micronucleus test to assess DNA damage in the erythrocytes of the European sea bass (Dicentrarchus labrax) exposed to environmental pollution in situ. Fish were collected from a fi sh farm in the Trogir Bay and their cages placed at an unpolluted reference site Šolta (Nečujam Bay) and a polluted site Vranjic (Kaštela Bay) for four weeks. A group of fi sh which remained at the fi sh farm Trogir Bay were used as the second control group. Fish exposed at the Vranjic site showed a signifi cantly higher erythrocyte DNA damage, measured by the comet assay, than either control group. Micronucleus induction showed a similar gradient of DNA damage, but did not reach statistical signifi cance. Our results show that cage exposure of a marine fi sh D. labrax can be useful in environmental biomonitoring and confi rm the comet assay as a suitable tool for detecting pollution-related genotoxicity.

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Assessment of environmental stress by the micronucleus test and the Comet assay on the genome of teleost populations from two natural environments

Cited by 137 publications

References 48 publications

Evaluation of genotoxic and oxidative stress response to dimethoate in freshwater fish Channa punctatus (Bloch)

Evaluation of genotoxic and oxidative stress response to dimethoate in freshwater fish Channa punctatus (Bloch)

Avaliação da mutagênese e genotoxicidade em Eigenmannia virescens (Teleostei: Gymnotiformes) expostos ao benzeno

Cage Exposure of European Sea Bass (Dicentrarchus Labrax) for in Situ Assessment of Pollution-Related Genotoxicity

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