Assessment of Evolutionary Affinities in Gossypium by Protein Electrophoresis

Johnson, B. Lennart; Thein, Maung Myint

doi:10.2307/2441274

Cited by 36 publications

(25 citation statements)

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“…The strains of Achromobacter, Chromobacterium, Erwinia, Mycoplana, Pseudomonas, Xanthomonas and Agrobacterium used are mentioned in previous papers (Kersters & De Ley, 1968;Kersters et al 1973;De Ley et al 1973) Culture conditions. All bacteria, except Zymomonas mobilis, were grown for 38 to 42 h at 28 "C in three Roux bottles on a solid medium containing (g/l tap water): glucose, 10; yeast extract (Nederlandse Gist en Spiritusfabriek, Brugge, Belgium), 10 ; (NH,)2S0,, I ; KH2P0,, 0.25; agar, 25.…”

Section: Methodsmentioning

confidence: 99%

“…Secondly, the patterns of closely related organisms were made completely reproducible by a mathematical procedure that we caIl the ' compensation method'. Johnson & Thein (1970) used a somewhat similar computerized treatment successfully with crude seed extracts of Gossypium. Increased sharpness and narrowness of protein bands could decrease the reproducibility if not corrected properly.…”

Section: Introductionmentioning

confidence: 99%

“…features (De Ley, 1972;Kersters, De Ley, Sneath & Sackin, 1973;De Ley, Tytgat, De Smedt & Michiels, 1973).…”

Section: Introductionmentioning

confidence: 99%

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Identification and Grouping of Bacteria Numerical Analysis of their Electrophoretic Protein Patterns

Kersters

Ley

1975

Journal of General Microbiology

217

121

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Improved methods for the identification and grouping of bacteria by polyacrylamide gel electrophoresis of soluble proteins are described. Electrophoretic protein patterns were obtained in rigorously standardized conditions. The results were much more reproducible than any described previously. Some of the factors affecting reproducibility were : growth conditions, time and speed of centrifugation of extracts, and conditions of gel electrophoresis. Protein patterns were compared by computing correlation coefficients from normalized densitometric tracings and clustering the strains by the unweighted average pair group method. As model systems, both Agrobacterium and Zymomonas were used because of differences in the sharpness of the peaks. The method was applied to 42 Agrobacterium strains. The agreement with the results of clustering by either phenotypic tests or DNA : DNA hybridization was excellent. Computerized comparisons of electrophoretic protein patterns can be a fast, easy and powerful tool for classification and identification of bacteria.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification and Grouping of Bacteria Numerical Analysis of their Electrophoretic Protein Patterns

Kersters

Ley

1975

Journal of General Microbiology

217

121

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“…In other words, zymographic difference tells us only about a limited aspect of genome differentiation. In this regard, electrophoretic study of bulk proteins, such as the seed protein studied by Johnson (1968) and Johnson et al (1970) in Triticum and Gossypium, is suitable for determining the genomic relationships among related species.…”

Section: Genomie Relationships Of Zymogramsmentioning

confidence: 99%

ISOZYME VARIATIONS IN AEGILOPS AND TRITICUM. I. ESTERASE ISOZYMES IN AEGILOPS STUDIED USING THE GEL ISOELECTROFOCUSING METHOD

Nakai

Tsunewaki

1971

The Japanese journal of genetics

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The study of isozyme variations has become a useful tool for investigating phylogenetic relationships among related species. Yoshitake (1969) reported the isozyme variation of acid phosphatase in the silkworm in relation to its speciation, while Chu (1967) and Shahi et al. (1969) compared isozymes of several enzymes among various species of the genus Oryza. Bhatia (1968) and Jaaska (1969Jaaska ( , 1970) studied esterase, acid phosphatase and alcohol dehydrogenase in some Triticum and Aegilops species. The gel isoelectrof ocusing method developed by Leaback and Rutter (1968) and others, gives a sharp zymogram, because carrier ampholites added to the gel form a pH gradient after electrophoresis and each isozyme is concentrated at a specific site, whose pH value corresponds to the isoelectric point of the respective isozyme. Consequently, this method effectively separates individual isozyme fractions by their specific isoelectric points along the pH gradient of the gel. Using this method, we previously investigated esterase isozymes in wheat and four Aegilops species (Nakai et al. 1969). Results encouraged us to extend the work to all species of Aegilops, whose genomic relationships have been thoroughly investigated (Kihara 1954(Kihara , 1957. Thus, the present experiment was carried out, in which a carrier ampholite with a pH range of 5-8 (a narrower range than that used in the previous work) was used, because most esterase isozymes in this genus have isoelectric points within this pH range.

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“…1975) and electrophero gram of seed protein (Johnson and Thein 1970), classifying G. bickii as a new G genome in Gossypium had a lot of scientific foundation.…”

Section: Results and Disscussionmentioning

confidence: 99%

Karyotype analysis of gossypium bickii prokh.

Ping¹

1990

CYTOLOGIA

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Assessment of Evolutionary Affinities in Gossypium by Protein Electrophoresis

Cited by 36 publications

References 0 publications

Identification and Grouping of Bacteria Numerical Analysis of their Electrophoretic Protein Patterns

Identification and Grouping of Bacteria Numerical Analysis of their Electrophoretic Protein Patterns

ISOZYME VARIATIONS IN <i>AEGILOPS</i> AND <i>TRITICUM</i>. I. ESTERASE ISOZYMES IN <i>AEGILOPS</i> STUDIED USING THE GEL ISOELECTROFOCUSING METHOD

Karyotype analysis of gossypium bickii prokh.

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