Assessment of exopolysaccharides, bacteriocins and in vitro and in vivo hypocholesterolemic potential of some Egyptian Lactobacillus spp.

Characterization of C-phycocyanin antioxidant, anti-inflammatory, anti-tumour, and anti-HCoV-229E activities and encapsulation for implementation in an innovative functional yogurt

“…The anti-proliferative activity on the HepG-2 (human liver carcinoma), HCT-116 (Human colorectal carcinoma), MCF-7 (human breast adenocarcinoma) and BJ-1 (normal human skin fibroblast) was estimated by the 3-[4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay. This test is based on MTT cleavage by mitochondrial dehydrogenases from viable cells [ 42 ]. Cells were placed in a 96-well sterile microplate (5 × 10 4 cells well −1 ) and incubated at 37 °C in serum-free media containing dimethyl sulfoxide (DMSO) and either a series of various concentrations of each sample or Doxorubicin (positive control) for 48 h before the MTT assay.…”

Optimization, Probiotic Characteristics, and Rheological Properties of Exopolysaccharides from Lactiplantibacillus plantarum MC5

“…The supernatant was collected, mixed with 95% alcohol (3:1 v / v ), and allowed to stand again at 4 °C for 24 h. The mixture was centrifuged, and the pellet was suspended in deionized water and dialyzed at 4 °C for 2 days using dialysis bags (molecular weights of 8–14 kDa). The total sugar content was detected using phenol-sulfate acid method [ 28 ]. Briefly, 0.1 g EPS was dissolved in 100 mL of distilled water, and 1 mL EPS solution was mixed well with 1 mL 6% ( w / v ) phenol solution and 5 mL 98% ( w / v ) H 2 SO 4 , shaken for 10 min, and the absorbance was determined at 490 nm.…”

“…RSR of DPPH free radical was assayed by El-Dein’ method [ 28 ]. A total of 2 mL of EPS solution (2, 4, 6, 8, and 10 mg/mL) was mixed with 2 mL 0.1 mMol/L DPPH solution.…”

Genome Analysis for Cholesterol-Lowing Action and Bacteriocin Production of Lactiplantibacillus plantarum WLPL21 and ZDY04 from Traditional Chinese Fermented Foods

“…Besides their cholesterol-lowering ability, their antagonistic capacity against pathogens is one of the important indexes for strengthening the probiotic properties of LABE strains. This capacity is mostly related to the production of organic acids and partially related to the yield of bacteriocin, one of the key secondary metabolites for partial LABE strains [ 13 , 14 ]. As a common species in TFFs, Lactobacillus spp.’s gene cluster and yield amount of bacteriocins depend on different niches.…”