Assessment of expression of NF-κB-related genes in periodontitis

Ghafouri‐Fard, Soudeh; Gholami, Leila; Nazer, Naghme; Hussen, Bashdar Mahmud; Shadnoush, Mahdi; Sayad, Arezou; Taheri, Mohammad

doi:10.1016/j.genrep.2021.101454

Cited by 4 publications

(3 citation statements)

References 18 publications

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“…The results of the current study are in line with other studies reporting abnormal expressions of immune-related genes in the blood of patients with periodontitis. For instance, two NF-κB-related genes, namely DILC and FBXL19-AS have been shown to be down-regulated both in blood samples of these patients compared with control samples [ 22 ]. A number of other long non-coding RNAs, namely Linc0116, Linc00667, CDK6-AS1, FENDRR and DIRC3 have been found to be down-regulated in the blood samples of patients with periodontitis [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

Altered expression of SOCS genes periodontitis

et al. 2022

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Suppressor of cytokine signalling (SOCS) family comprises a group of proteins that impede JAK/STAT signalling, thus being involved in the pathogenesis of immune-related conditions. In the present work, we aimed at identification of the role of SOCS genes in the pathogenesis of periodontitis through evaluation of their expression levels both in the circulation and in the affected tissues of patients. Thus, we measured expression levels of SOCS1-3 and SOCS5 transcripts in the blood and gingival samples of patients with periodontitis in comparison with control samples obtained during dental crown lengthening. Expressions of SOCS1, SOCS2, SOCS3 and SOCS5 genes were similar between gingival tissues of patients and controls. However, our results demonstrated under-expression of SOCS1 in blood samples of patients compared with controls (Ratio of mean expression (RME) = 0.47, P value = 0.04). The same pattern was observed among female subjects (RME = 0.38, P value = 0.04). SOCS2 was down-regulated in blood samples of female patients compared with female controls (RME = 0.22, P value = 0.04). SOCS3 was also under-expressed in the circulation of total cases versus total controls (RME = 0.29, P value = 0.02) and in female patients compared with female controls (RME = 0.19, P value = 0.04). Expression of SOCS5 was not different between blood samples two study groups. SOCS2 had the best function in separation of affected tissues from unaffected ones (AUC = 0.66, sensitivity = 0.39, specificity = 0.83). SOCS3 was superior to other transcripts in differentiation of blood samples of patients from normal blood samples (AUC = 0.69, sensitivity = 0.81, specificity = 0.68). Combination of transcript levels of SOCS1, SOCS2, SOCS3 and SOCS5 genes enhanced the AUC values to 0.64 and 0.67 in tissue and blood specimens, respectively. Taken together, certain SOCS genes have been found to be dysregulated in the circulation of patients with periodontitis.

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Section: Discussionmentioning

confidence: 99%

Altered expression of SOCS genes periodontitis

et al. 2022

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“…Consistent with our finding, CEBPA, an NF- κ B-related associated gene, has been previously reported in a bioinformatics study as a key TF involved in H. pylori-mediated immune deregulation [ 71 , 72 ] and validated as downregulated in infected gastric tissues [ 73 ]. In periodontitis, lower serum levels of CEBPA have been reported [ 74 ]. The RUNX genes are important regulators of cellular development, and reduced RUX1 expression in gastric cancer cell lines is documented [ 75 ].…”

Section: Discussionmentioning

confidence: 99%

Integrative Analysis of Deregulated miRNAs Reveals Candidate Molecular Mechanisms Linking H. pylori Infected Peptic Ulcer Disease with Periodontitis

Ning

Wang

2022

Disease Markers

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Objective. Periodontitis is a highly prevalent oral infectious disease and has been increasingly associated with H. pylori infection, gastric inflammation, and gastric cancer but little is known about epigenetic machinery underlying this potentially bidirectional association. The present study is aimed at identifying key deregulated miRNA, their associated genes, signaling pathways, and compounds linking periodontitis with H. pylori-associated peptic ulcer disease. Methods. miRNA expression datasets for periodontitis-affected and H. pylori-associated peptic ulcer disease-affected tissues were sought from the GEO database. Differentially expressed miRNA (DEmiRNAs) were identified and the overlapping, shared-DEmiRNA between both datasets were determined. Shared-DEmiRNA-target networks construction and functional analyses were constructed using miRNet 2.0, including shared-DEmiRNA-gene, shared-DEmiRNA-transcription factor (TF), and shared-DEmiRNA-compound networks. Functional enrichment analysis for shared DEmiRNA-gene and shared DEmiRNA-TF networks was performed using the KEGG, Reactome, and Geno Ontology (GO) pathways. Results. 11 shared-DEmiRNAs were identified, among which 9 showed similar expression patterns in both diseases, and 7 were overexpressed. miRNA hsa-hsa-mir-155-5p and hsa-mir-29a-3p were top miRNA nodes in both gene and TF networks. The topmost candidate miRNA-deregulated genes were PTEN, CCND1, MDM2, TNRC6A, and SCD while topmost deregulated TFs included STAT3, HIF1A, EZH2, CEBPA, and RUNX1. Curcumin, 5-fluorouracil, and the gallotanin 1,2,6-Tri-O-galloyl-beta-D-glucopyranose emerged as the most relevant linkage compound targets. Functional analyses revealed multiple cancer-associated pathways, PI3K pathways, kinase binding, and transcription factor binding among as enriched by the network-associated genes and TFs. Conclusion. Integrative analysis of deregulated miRNAs revealed candidate molecular mechanisms comprising of top miRNA, their gene, and TF targets linking H. pylori-infected peptic ulcer disease with periodontitis and highlighted compounds targeting both diseases. These findings provide basis for directing future experimental research.

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“…The results showed that MMP-9 is significantly linked to the risk of getting periodontitis. Also, nuclear factor kappa B (NF-κB) is important for the swelling and irritation that affects the gums and alveolar bone around teeth, especially when gum diseases like gingivitis and periodontitis are present ( Ghafouri-Fard et al., 2022 ). In certain conditions, elevated NF-κB expression can exacerbate inflammation ( Arabaci et al., 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Brazilin cream from Caesalpinia sappan inhibit periodontal disease: in vivo study

Sugiaman,

Jeffrey,

Naliani

et al. 2024

PeerJ

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Background Gingivitis is an inflammation of the gums that is the initial cause of the development of periodontal disease by the activity of Nuclear Factor-kappa B (NF-κB), Interleukin-1β (IL-1β), Interleukin-6 (IL-6), p38, and Tumor Necrosis Factor-α (TNF-α). Unaddressed chronic inflammation can lead to persistent disturbances in other parts of the body. Brazilin is a naturally occurring plant chemical that may have antibacterial and anti-inflammatory effects. Treatment based on the natural plant compound, brazilin, is developed in the form of a topical cream for easy application. Objective The aim is to develop the natural compound brazilin in the form of a topical cream as an anti-inflammatory agent to reduce NF-κB expression through Imunohistochemistry (IHC) methods, and the expression of pro-inflammatory genes IL-1β, IL-6, p38, and TNF-α. Methods Male Sprague-Dawley rats were induced with gingivitis using P. gingivalis bacteria. The observed groups included rats treated with a single application of brazilin cream and rats treated with two applications of brazilin cream. The treatment was administered for 15 days. On days 3, 6, 9, 12, and 15, anatomical wound observations and wound histology using hematoxylin-eosin and Masson’s Trichrome staining were performed. NF-κB protein expression was analyzed using the IHC method. Gingival inflammation gene expression of NF-κB, IL-1β, IL-6, p38, and TNF-α was measured using q-RTPCR. Results Single and double applications of brazilin cream increased angiogenesis and decreased NF-κB protein expression, in addition to the IL-1β, IL-6, p38, and TNF-α gene expressions. Conclusion In a rat gingivitis model, Brazilin cream may function as an anti-inflammatory agent in the gingival tissue.

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Assessment of expression of NF-κB-related genes in periodontitis

Cited by 4 publications

References 18 publications

Altered expression of SOCS genes periodontitis

Altered expression of SOCS genes periodontitis

Integrative Analysis of Deregulated miRNAs Reveals Candidate Molecular Mechanisms Linking H. pylori Infected Peptic Ulcer Disease with Periodontitis

Brazilin cream from Caesalpinia sappan inhibit periodontal disease: in vivo study

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