Assessment of extracellular antibiotic resistance genes (eARGs) in typical environmental samples and the transforming ability of eARG

Dong, Peiyan; Wang, Hui; Fang, Tingting; Wang, Yun; Ye, Quanhui

doi:10.1016/j.envint.2019.01.050

Cited by 203 publications

(82 citation statements)

References 41 publications

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“…The presence of antibiotic residues, even at subinhibitory concentrations, can stimulate HGT events and ARG transfer [105]. Dong et al showed that extracellular ARGs, such as those detected in manure, maintain their transforming ability [106]. Animal manure represents a major source of antibiotics and ARGs in the environment [107], creating a favorable environment for HGT events [37,48].…”

Section: Args Spread By Hgt Mechanismsmentioning

confidence: 99%

Manure as a Potential Hotspot for Antibiotic Resistance Dissemination by Horizontal Gene Transfer Events

Lima

Domingues

Silva

2020

Veterinary Sciences

137

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The increasing demand for animal-derived foods has led to intensive and large-scale livestock production with the consequent formation of large amounts of manure. Livestock manure is widely used in agricultural practices as soil fertilizer worldwide. However, several antibiotic residues, antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria are frequently detected in manure and manure-amended soils. This review explores the role of manure in the persistence and dissemination of ARGs in the environment, analyzes the procedures used to decrease antimicrobial resistance in manure and the potential impact of manure application in public health. We highlight that manure shows unique features as a hotspot for antimicrobial gene dissemination by horizontal transfer events: richness in nutrients, a high abundance and diversity of bacteria populations and antibiotic residues that may exert a selective pressure on bacteria and trigger gene mobilization; reduction methodologies are able to reduce the concentrations of some, but not all, antimicrobials and microorganisms. Conjugation events are often seen in the manure environment, even after composting. Antibiotic resistance is considered a growing threat to human, animal and environmental health. Therefore, it is crucial to reduce the amount of antimicrobials and the load of antimicrobial resistant bacteria that end up in soil.

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Section: Args Spread By Hgt Mechanismsmentioning

confidence: 99%

Manure as a Potential Hotspot for Antibiotic Resistance Dissemination by Horizontal Gene Transfer Events

Lima

Domingues

Silva

2020

Veterinary Sciences

137

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“…The quantification of ARGs in soils amended with livestock and swine manure (Brooks et al, 2014;Tao et al, 2014) was performed with high-throughput qPCR assay (Rocha et al, 2018;Blau et al, 2019). In a recent study, both intracellular and extracellular DNA containing ARGs were quantified in sludge at about 10 10 and 10 12 copies per gram, respectively (Dong et al, 2019). Here, the intracellular ARGs were assessed through conjugation with cell-cell contact, whereas the extracellular ARGs were assessed through natural transformation.…”

Section: Different Approaches To Resistome Profiling Studymentioning

confidence: 99%

Exploring the Animal Waste Resistome: The Spread of Antimicrobial Resistance Genes Through the Use of Livestock Manure

et al. 2020

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Antibiotic resistance is a public health problem of growing concern. Animal manure application to soil is considered to be a main cause of the propagation and dissemination of antibiotic residues, antibiotic-resistant bacteria (ARB), and antibiotic resistance genes (ARGs) in the soil-water system. In recent decades, studies on the impact of antibioticcontaminated manure on soil microbiomes have increased exponentially, in particular for taxonomical diversity and ARGs' diffusion. Antibiotic resistance genes are often located on mobile genetic elements (MGEs). Horizontal transfer of MGEs toward a broad range of bacteria (pathogens and human commensals included) has been identified as the main cause for their persistence and dissemination. Chemical and bio-sanitizing treatments reduce the antibiotic load and ARB. Nevertheless, effects of these treatments on the persistence of resistance genes must be carefully considered. This review analyzed the most recent research on antibiotic and ARG environmental dissemination conveyed by livestock waste. Strategies to control ARG dissemination and antibiotic persistence were reviewed with the aim to identify methods for monitoring DNA transferability and environmental conditions promoting such diffusion.

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“…Consequently, the occurrence of eDNA has been studied in various habitats, including soil, sediments, freshwater and marine ecosystems [ 17 ]. Both live and dead cells contribute to the release of eDNA as fraction to the environment in the different processes of secretion and lysis, respectively [ 18 , 19 ]. Given the long persistence of eDNA in sediment and soil samples for years, it is of utmost importance to study the role of eDNA in the spread of ARGs in the environmental matrices [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Extracellular DNA (eDNA): Neglected and Potential Sources of Antibiotic Resistant Genes (ARGs) in the Aquatic Environments

Sivalingam

Poté

Prabakar³

2020

Pathogens

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Over the past decades, the rising antibiotic resistance bacteria (ARB) are continuing to emerge as a global threat due to potential public health risk. Rapidly evolving antibiotic resistance and its persistence in the environment, have underpinned the need for more studies to identify the possible sources and limit the spread. In this context, not commonly studied and a neglected genetic material called extracellular DNA (eDNA) is gaining increased attention as it can be one of the significant drivers for transmission of extracellular ARGS (eARGs) via horizontal gene transfer (HGT) to competent environmental bacteria and diverse sources of antibiotic-resistance genes (ARGs) in the environment. Consequently, this review highlights the studies that address the environmental occurrence of eDNA and encoding eARGs and its impact on the environmental resistome. In this review, we also brief the recent dedicated technological advancements that are accelerating extraction of eDNA and the efficiency of treatment technologies in reducing eDNA that focuses on environmental antibiotic resistance and potential ecological health risk.

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Assessment of extracellular antibiotic resistance genes (eARGs) in typical environmental samples and the transforming ability of eARG

Cited by 203 publications

References 41 publications

Manure as a Potential Hotspot for Antibiotic Resistance Dissemination by Horizontal Gene Transfer Events

Manure as a Potential Hotspot for Antibiotic Resistance Dissemination by Horizontal Gene Transfer Events

Exploring the Animal Waste Resistome: The Spread of Antimicrobial Resistance Genes Through the Use of Livestock Manure

Extracellular DNA (eDNA): Neglected and Potential Sources of Antibiotic Resistant Genes (ARGs) in the Aquatic Environments

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