Assessment of Extrapulmonary Toxicity Induced by Carbon Nanomaterials Following Intra-Tracheal Instillation in Rats

Angoth, Bhikku; Lingabathula, Harikiran; Yellu, Narsimha Reddy

doi:10.22159/ajpcr.2017.v10i5.17152

Cited by 2 publications

(2 citation statements)

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“…Irregular polygonal shape hepatocytes of liver cells of Heteropneustes fossilis exposed to Cypermethrin [59]. Dosedependent toxicity of liver and kidney tissues was observed in the intratracheal installation of carbon nanomaterials in rats [60]. Vacuoles, Pyknosis, and Necrosis were evident in the experimental groups.…”

Section: Discussionmentioning

confidence: 99%

Ecotoxicology of Green Synthesized Silver Nanoparticles on Fresh Water Fish Mystus Gulio

Abirami

Jose

Govindarajulu

et al. 2017

Int J Pharm Pharm Sci

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Objective: Nanotechnology an advanced tool to synthesis atomic level particles. Increased application of silver nanoparticles results in the bioaccumulation of these particles in the environment. The biological effect of the green synthesized silver nanoparticles on the fresh water cat fish ws studied in the present study.Methods: Asystasia gangetica leaf extract was used to synthesize silver nanoparticles, the particles were characterized by scanning electron microscopy. Fifteen days after the introduction of fishes into the medium containing AgNPs, the fishes were sacrificed and the tissues were processed for biochemical and histological studies. Na + -K + Results:The results observed in the present study reveals a marked difference in the level of carbohydrate in the muscle tissue from 48.338±0.320 to 22.747±1.837 µg/100 mg wet tissue followed by gills and liver with 41.21% and 25.48% decrease. Total protein content was decreased from 649.094±1.429 to 491.56±0.925 µg/mg wet tissues. The drastic increase in the total lipids was observed in liver tissue of treated fishes from 0.240±0.004 to 0.408±0.005 mg/gm wet tissue. Na -ATPase was estimated using LeBel method. Estimation of carbohydrate was done by the Phenol-sulphuric acid method. Total protein was estimated using Lowry's method, Sulpho-Vanilline method was used to estimate the total lipids. + -K + Conclusion:The plant-mediated synthesized AgNPs shows a potential toxic effect on all the tissues studied; changes were observed in the normal architecture of tissues as well as in the biochemical parameters. To understand the mechanism of toxicity of these particles further studies at the molecular level has to be carried out.-ATPase activity of liver tissue increased from 0.153±0.001 to 0.225±0.003 in the liver tissue of treated fishes, followed by muscles and gills with 33.61% and 35% of the increase in the activity. Marked changes in the structure of gills with degenerated primary gill lamellae, necrosis, hyperplasia and fused primary lamellae were evident in experimental group. Necrosis, vacuolization, disintegrated nucleus and blood sinusoids were observed in liver tissues. Disintegration of myofibrils was evident in AgNP treated group of fishes.

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Section: Discussionmentioning

confidence: 99%

Ecotoxicology of Green Synthesized Silver Nanoparticles on Fresh Water Fish Mystus Gulio

Abirami

Jose

Govindarajulu

et al. 2017

Int J Pharm Pharm Sci

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“…After 1 day, 1 week, 1 month, and 3 months after exposure, the characteristics of CNM were tested by dripping into the rat trachea, by using serum biochemical parameters (such as alanine aminotransferase (ALT) and creatinine) and diagnostic assays kit to assess extrapulmonary toxicity. His research process lacks actual control [4]. Carbon nanomaterials (CNMs), including carbon nanotubes (CNTs) and their derivatives, have a variety of technical and biomedical applications.…”

Section: Introductionmentioning

confidence: 99%

Plane Animation Simulation of the Interaction between Carbon Nanomaterials and Cell Lysosomes

Wen

Qiu

Zeng

2020

Journal of Chemistry

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With the continuous development of cell dynamics, Raman scattering has become more and more common in the application of cell imaging and dynamic changes in chemical substances. This research mainly discusses the plane animation simulation process of the interaction between carbon nanomaterials and cell lysosomes. Twenty parallel HeLa cells were seeded on 40 mm imaging scaffolds. The 15% bovine placental serum cell culture cells are placed in a thermostat for 12 hours at a CO2 concentration of 6%. After washing 4 times, 20 μL of the dual control system is added to the confocal dish, and cycle optimization culture is performed at 2, 4, 6, and 8 hours. It is incubated for 10 hours, 20 hours, and 30 hours (35°C, 6% CO2). Next, the HeLa cells were taken out and seeded on three 30 mm confocal cell culture dishes. CCl4 is added to the initial confocal Petri dish. After heating for 30 minutes, the nanoparticle system is added to the two confocal Petri dishes and circulated within an appropriate time. After washing with PBS, the SERS signal in the cells was imaged with a laser confocal Raman microscope, and the excitation channels were GFP 475 and Cy3 channels. LysoTrackerRed (2p μM) was used for local experiments of cell isotope. Deoxygen (2p μM) was used to induce cell death, and the pH changes in lysosomes in cells were imaged in real time with a confocal microscope. Two distinct peaks in the Raman spectrum were observed at 1246 cm−1 and 1543 cm−1. The research results show that the carbon nanomaterial synthesized by a simple method at room temperature has high stability and is suitable for analysis and detection of imaging with cells as the target.

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Assessment of Extrapulmonary Toxicity Induced by Carbon Nanomaterials Following Intra-Tracheal Instillation in Rats

Cited by 2 publications

References 20 publications

Ecotoxicology of Green Synthesized Silver Nanoparticles on Fresh Water Fish Mystus Gulio

Ecotoxicology of Green Synthesized Silver Nanoparticles on Fresh Water Fish Mystus Gulio

Plane Animation Simulation of the Interaction between Carbon Nanomaterials and Cell Lysosomes

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