Assessment of Fitness Effects Associated with Phosphine Resistance in<i>Rhyzopertha dominica</i>(F.) (Coleoptera: Bostrichidae) and<i>Tribolium castaneum</i>(Herbst) (Coleoptera: Tenebrionidae)

Bajracharya, N.S.; Opit, George P.; Talley, Justin L.; Gautam, S. G.; Payton, Mark E.

doi:10.4001/003.024.0039

Cited by 9 publications

(6 citation statements)

References 23 publications

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“…The earliest reports of resistance to phosphine in the field were made in 1972-1973 7 , but since that time, phosphine resistance has been reported in a whole host of the most common stored-product insect pests, including Tribolium castaneum 8 – 12 , Rhyzopertha dominica 8 , 9 , 11 , 12 , Sitophilus oryzae 13 – 15 , Lasioderma serricorne 16 , Sitophilus zeamais 17 , Oryzaephilus surinamensis 12 , 18 , C. ferrugineus 4 , 5 and Liposcelis bostrychophila 19 . Despite several decades of study, the biological mechanisms underlying resistance to phosphine and its toxicity are not well understood 20 .…”

Section: Introductionmentioning

confidence: 99%

Reference gene selection to determine differences in mitochondrial gene expressions in phosphine-susceptible and phosphine-resistant strains of Cryptolestes ferrugineus, using qRT-PCR

Tang

Duan

et al. 2017

Sci Rep

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Cryptolestes ferrugineus is a serious pest of stored grain and has developed high levels of resistance to phosphine fumigants in many countries. Measuring differences in expression levels of certain ‘resistant’ genes by quantitative real-time PCR (qRT-PCR) may provide insights into molecular mechanisms underlying resistance to phosphine in C. ferrugineus, but reliable qRT-PCR results depend on suitable reference genes (RGs). We evaluated the stability of nine candidate RGs across different developmental stages and phosphine strains of C. ferrugineus, using four softwares. The results showed that RPS13 and EF1α were the most stable RGs, whereas α-TUB was the least under developmental stages. Across the different strains, RPS13 and γ-TUB were the most stable RGs, whereas CycA and GAPDH were the least. We confirmed the reliability of the selected RGs by qRT-PCR analyses of the mitochondrial cox1 gene. Expression of cox1 was not significantly different in the phosphine-resistant strain compared with the phosphine-susceptible strain, but three mitochondrial genes (nad3, atp6 and cob) were significantly down-regulated. These results suggest that alterations in the expressions of these three genes may be associated with phosphine resistance in C. ferrugineus. The findings will facilitate future functional genomics studies on the development and phosphine resistance in C. ferrugineus.

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Section: Introductionmentioning

confidence: 99%

Reference gene selection to determine differences in mitochondrial gene expressions in phosphine-susceptible and phosphine-resistant strains of Cryptolestes ferrugineus, using qRT-PCR

Tang

Duan

et al. 2017

Sci Rep

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“…Taken these reports into consideration, it can be concluded that establishing a near-isogenic resistant insect strain is crucial in investigating fitness costs associated with an insecticide resistance trait. Several earlier studies reported significant fitness cost associated with phosphine resistance in T. castaneum, R. dominica and O. surinamensis (Pimentel et al 2007;Sousa et al 2009;Bajracharya et al 2016) but without controlling for genetic background. Based on our present results, we believe that such results need to be viewed with caution and suggest that future investigations on fitness should include near-isogenic resistant lines.…”

Section: Discussionmentioning

confidence: 94%

“…Fitness costs associated with phosphine resistance in stored product insects have been well researched and involved different approaches. Several studies established fitness cost associated with phosphine resistance in major pest species by comparing their biological parameters, such as reproduction and developmental time, particularly in the resistant and susceptible populations of the red flour beetle Tribolium castaneum (Herbst), the lesser grain borer, Rhyzopertha dominica (F.), and the saw-toothed grain beetle, Oryzaephilus surinamensis (L.) (Pimentel et al 2007;Sousa et al 2009;Bajracharya et al 2016). In these studies, the researchers looked for significant relationships between the biological parameters and the resistance factor of the population.…”

Section: Introductionmentioning

confidence: 99%

Gene introgression in assessing fitness costs associated with phosphine resistance in the rusty grain beetle

et al. 2021

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The current study investigates the fitness cost associated with phosphine resistance in the rusty grain beetle, Cryptolestes ferrugineus (Stephens), a problematic pest in the stored commodities that has developed strong resistance to fumigant phosphine. Three characterised insect strains: the susceptible (Ref-S), the strongly resistant (Ref-R), the introgressed resistant (Intro-R) and a segregating population (F 25 ) derived from crossing the Ref-S and Ref-R strains were used in this study. Intro-R was developed by introgressing two phosphine resistance genes, cf_rph1 and cf_rph2 into Ref-S, aimed to reduce the influence of background genetic factors. Intro-R exhibited 592 × resistance to phosphine and homozygous for strong resistance allele, cf_rph2 (L73N). Two key fitness cost criteria, developmental time and fecundity, were assessed under optimal and suboptimal conditions (less favourable diet and low temperature). There was no significant difference in developmental time and fecundity between Ref-S and either Intro-R strain or F 25 under optimal conditions. When challenged with a less favourable diet, cracked wheat + cracked sorghum (CW + CS), or exposed to a low temperature (22 °C), both Intro-R and Ref-S had similar developmental time and total numbers of F 1 progeny, confirming the absence of significant fitness effects expressed in these conditions. Therefore, we conclude that strongly phosphine resistant C. ferrugineus are unlikely to incur potential fitness costs. This finding will have implications towards developing strategies to manage this pest.

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“…A common way to control stored product pests is to use gaseous chemical insecticides, such as phosphine (Bajracharya et al . 2016; Collins et al . 2017), and broad‐spectrum organophosphorus insecticides (Rumbos et al .…”

Section: Introductionmentioning

confidence: 99%

Population development ofRhyzopertha dominica(F.) (Coleoptera: Bostrichidae) on different stored products

Ren,

Wang,

Wang

et al. 2023

Entomological Research

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The lesser grain borer, Rhyzopertha dominica, is an important pest of various stored products around the world. In this study, the development, survival, reproduction, and life table parameters of R. dominica were investigated on six stored products (angelica, jujube, maize, rice, soybean, and wheat). The developmental time of the immature stage of R. dominica was shortest on wheat (40.20 days) and longest on angelica (67.04 days). The survival rate of the immature stage was highest on wheat (76.33%) and lowest on angelica (41.00%). The fecundity level of R. dominica was highest on wheat (246.05 eggs/female) and lowest on angelica (69.38 eggs/female). The net reproductive rate (R0) and intrinsic rate of increase (rm) of R. dominica differed significantly among the six stored products. The highest R0 of R. dominica was on wheat (68.50), followed by rice (41.28), maize (32.32), soybean (27.17), jujube (23.16), and angelica (20.18); the rm values showed a similar trend, with values of 0.059, 0.046, 0.042, 0.039, 0.036, and 0.033, respectively. Our results indicate that wheat was the most suitable stored product, whereas angelica was the least suitable, for the feeding, development, and population increase of R. dominica. These findings provide basic information about the occurrence trends and characteristics of R. dominica that will be useful for the control of this pest on different stored products. The physicochemical properties of angelica should be further explored for potential application in the control or integrated management of R. dominica.

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Assessment of Fitness Effects Associated with Phosphine Resistance inRhyzopertha dominica(F.) (Coleoptera: Bostrichidae) andTribolium castaneum(Herbst) (Coleoptera: Tenebrionidae)

Cited by 9 publications

References 23 publications

Reference gene selection to determine differences in mitochondrial gene expressions in phosphine-susceptible and phosphine-resistant strains of Cryptolestes ferrugineus, using qRT-PCR

Reference gene selection to determine differences in mitochondrial gene expressions in phosphine-susceptible and phosphine-resistant strains of Cryptolestes ferrugineus, using qRT-PCR

Gene introgression in assessing fitness costs associated with phosphine resistance in the rusty grain beetle

Population development ofRhyzopertha dominica(F.) (Coleoptera: Bostrichidae) on different stored products

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