Assessment of flavonoids contents and in vitro antioxidant activity of Launaea procumbens

Khan, Rahmat Ali; Khan, Muhammad Rashid; Sahreen, Sumaira; Ahmed, Mushtaq

doi:10.1186/1752-153x-6-43

Cited by 169 publications

(111 citation statements)

References 49 publications

Supporting

Mentioning

100

Contrasting

Order By: Relevance

“…In both leaf extracts, there was a dose-dependent increase in total antioxidant activity. Many flavonoids and related polyphenols contribute significantly to the phosphomolybdate scavenging activity of medicinal plants [52,53]. The present study was in agreement with the above findings and indicated that the presence of significant levels of non-enzymic antioxidants, namely, flavonoids and polyphenols in leaf extracts of Alternanthera plants might contribute to the phosphomolybdate scavenging activity which was measured as TAC.…”

Section: Tacsupporting

confidence: 80%

Phytochemical Screening and in Vitro Antioxidant Activities of Leaf Extracts of Alternanthera Brasiliana (L). Kuntze and Alternanthera Bettzickiana Regel.

Kasthuri¹,

Ramesh²

2018

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Objectives: The present study was carried out to determine the phytochemical constituents and in vitro antioxidant activities of leaf extracts of Alternanthera brasiliana (L). Kuntze (A. brasiliana) and Alternanthera bettzickiana regel (A. bettzickiana).Methods: Preliminary phytochemical screening was performed in petroleum ether, chloroform, acetone, ethanol, hydroethanolic and water extracts of leaves of A. brasiliana and A. bettzickiana. The level of alkaloids, flavonoids, total phenolic content, tannins, Vitamin C, Vitamin E, GSH, and total proteins were determined in hydroethanolic, ethanol, and water extracts. Antioxidant activity of the hydroethanolic leaf extracts of A. brasiliana and A. bettzickiana were determined by 2,2-diphenyl-1-picryl-hydrazyl-hydrate free radical scavenging assay, nitric oxide scavenging assay, superoxide anion scavenging assay, ferric reducing antioxidant power assay, total antioxidant capacity, and reducing power assay. Results:The phytochemical screening of six different extracts of A. brasiliana and A. bettzickiana revealed the presence of various phytonutrients. Quantitative analysis of secondary metabolites in ethanol, hydroethanolic and water extracts of leaves of A. brasiliana and A. bettzickiana showed the presence of high amount of secondary metabolites in the hydroethanolic extract. In vitro, antioxidant assay of two plant extracts revealed that A. bettzickiana was more potent than A. brasiliana in scavenging free radicals. Conclusion:The different extracts from A. brasiliana and A. bettzickiana and specifically the hydroethanolic extract of A. bettzickiana revealed several properties such as rich source of phytonutrients, higher free radical scavenging properties, and significant antioxidant capabilities. Therefore, the bioactive compound should be isolated in future studies and could be used as a safe and serve as a potential source of natural antioxidants.

show abstract

Section: Tacsupporting

confidence: 80%

Phytochemical Screening and in Vitro Antioxidant Activities of Leaf Extracts of Alternanthera Brasiliana (L). Kuntze and Alternanthera Bettzickiana Regel.

Kasthuri¹,

Ramesh²

2018

Asian J Pharm Clin Res

View full text Add to dashboard Cite

show abstract

“…Flavonoids are the chief of phenolic compounds and they also have potent antioxidant activities with positive effects on human health [33]. The total flavonoid content in leaves of Q. salicina and Q. serrata were much higher than that of Launaea procumbens which as a food supplement in Pakistan (4 -13 mg RE/g DW) [34]. From the results, this study concluded that leaves of Q. salicina and bark of Q. serrata may obtain rich phenolics and should be further elaborated to exploit their biological properties.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Capacity and Phenolic Contents of Three <i>Quercus</i> Species

Tuyen

Khang

et al. 2016

ILNS

View full text Add to dashboard Cite

ABSTRACT. The antioxidant capability and phenolic contents of ethanol extracts (free phenolics) and ethyl acetate extracts (bound phenolics) of three Quercus species were estimated in this work. The antioxidant activities were examined by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) free radical, reducing power and -carotene bleaching methods. HPLC was employed to detect major phenolic acids. The leaf extract of Q. salicina contained maximum total phenolics while the highest total flavonoid content was found in the leaf extract of Q. serrata. The antioxidant activities varied among three species. Bark extract of Q. salicina was the most potential and it was closed to levels of the standard antioxidative dibutyl hydroxytoluene (BHT). The bark extract of Q. serrata also showed promising antioxidant activities despite their eminence was negligibly lower than Q. salicina. Stronger antioxidant activities of free phenolics than those of the bound phenolics may be attributed to higher quantities of free phenolics in the barks of Quercus species, however total flavonoids may not contribute a critical role. By HPLC analysis, thirteen phenolic acids were detected in the leaf and bark extracts. Of them, Q. salicina showed maximum in number (ten compounds) and quantities of detected phenolic acids. Ellagic, chlorogenic and benzoic acids were dominant in Quercus species. Findings of this study revealed that leaves and barks of three Quercus species are rich source of antioxidants, and Q. salicina is the most promising and should be elaborated to exploit its pharmaceutical properties.

show abstract

“…The antioxidant capacity of the fractions was measured spectrophotometrically through phosphomolybdenum method, based on the reduction of Mo (VI) to Mo (V) by the test sample and the consequent formation of green phosphate/Mo (V) compounds. Recent studies have shown that numerous flavonoid and related polyphenols contribute significantly to the phosphomolybdate scavenging activity of medicinal plants (Sharififar et al, 2009;Khan et al, 2012). Regarding the result of total antioxidant activity, the methanol and chloroform fractions of R. dumetorum contain antioxidant compounds as equivalents of ascorbic acid to efficiently decrease the oxidant in the reaction matrix.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant potential and cytotoxicity of Randia dumetorum Lam. leaf extract

Abdullah-Al-Ragib¹,

Hossain²,

Hossain³

et al. 2017

J. Pharmacognosy Phytother.

View full text Add to dashboard Cite

The aim of the current study was to explore the antioxidant potential and cytotoxicity of different 'fractions from the leaf extract of Randia dumetorum. Proximate analysis and phytochemical screening were done with standard protocol. The antioxidant activity was evaluated by using reducing power assay, total antioxidant capacity determination, determination of total phenolic content, determination of total flavonoid content and reduction of ferric ions by ortho-phenanthroline color method. In the antioxidant studies, ascorbic acid, gallic acid and butylated hydroxy toluene (BHT) were used as standard antioxidant compound. The brine shrimp lethality test was used to examine cytotoxicity. In the proximate analysis, moisture content, total ash value, acid insoluble ash and water-soluble ash value were found in the leaf of R. dumetorum. Concerning the phytochemical screening, there was the presence of glycosides, flavonoids, reducing sugars, saponins, phenolic compounds, tannins on different fractions, but the absence of gums and mucilage, alkaloid, protein, and amino acid. The results show that all the extracts of R. dumetorum leaf possess significant antioxidant activity. 'The methanol and water extracts of the leaves showed significant cytotoxic activity as compared to dimethyl sulfoxide (DMSO) (LC 50 1.07 µg/ml). These findings suggest that R. dumetorum leaves might be a good antioxidants source and possess mild cytotoxic effect.

show abstract

Assessment of flavonoids contents and in vitro antioxidant activity of Launaea procumbens

Cited by 169 publications

References 49 publications

Phytochemical Screening and in Vitro Antioxidant Activities of Leaf Extracts of Alternanthera Brasiliana (L). Kuntze and Alternanthera Bettzickiana Regel.

Phytochemical Screening and in Vitro Antioxidant Activities of Leaf Extracts of Alternanthera Brasiliana (L). Kuntze and Alternanthera Bettzickiana Regel.

Antioxidant Capacity and Phenolic Contents of Three <i>Quercus</i> Species

Antioxidant potential and cytotoxicity of Randia dumetorum Lam. leaf extract

Contact Info

Product

Resources

About