Assessment of Four Commonly Employed in Vitro Arsenic Bioaccessibility Assays for Predicting in Vivo Relative Arsenic Bioavailability in Contaminated Soils

Juhasz, Albert L.; Weber, John; Smith, Euan; Naidu, Ravi; Rees, Matthew; Rofe, Allan M.; Kuchel, Tim; Sansom, Lloyd

doi:10.1021/es902427y

Cited by 175 publications

(227 citation statements)

References 28 publications

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“…In addition, organic constituents in the gastric phase can influence the solubility of As and Fe . Nevertheless, soil properties (i.e., total As concentration, particle size, and mineral phases) may also affect the As bioaccessibility, as reported in previous studies (Cui and Chen, 2011;Juhasz et al, 2009;Smith et al, 2014;Yang et al, 2003;Yin et al, 2015a). Presumably, the differences in As bioaccessibility are a result of synergistic effects between the in vitro parameters and soil properties.…”

Section: Arsenic Speciation Analysismentioning

confidence: 70%

“…The disparity in the As bioaccessibility may be in part reflective of the differences in parameters (i.e., gastric pH, and constituents) for the in vitro methods. The lower pH in the gastric phase with higher As bioaccessibility leads to an increase in the dissolution of As and Fe (Juhasz et al, 2009;Ruby et al, 1996). In addition, organic constituents in the gastric phase can influence the solubility of As and Fe .…”

Section: Arsenic Speciation Analysismentioning

confidence: 99%

“…Human health risk assessment depends mainly on in vivo bioavailability methods and in vitro bioaccessibility methods. Generally, bioaccessibility is defined as the fraction of As that is soluble in the gastrointestinal environment of human being and available for absorption, whereas bioavailability is determined as the fraction of As that is absorbed into the systemic circulation (Juhasz et al, 2009;Ruby et al, 1999). In view of the time, cost and ethical considerations associated with in vivo methods, in vitro methods are widely used in assessing human health risk.…”

Section: Introductionmentioning

confidence: 99%

“…In view of the time, cost and ethical considerations associated with in vivo methods, in vitro methods are widely used in assessing human health risk. For As contaminated soils, a strong linear relationship between As relative bioavailability (RBA) and As bioaccessibility has been reported Juhasz et al, 2009Juhasz et al, , 2014Rodriguez and Basta, 1999;Ruby et al, 1996). By establishing good in vivo-in vitro correlations, these in vitro bioaccessibility methods have been proposed as surrogates to predict the RBA for refining the risk to human health of inadvertent oral soil ingestion as an important exposure route.…”

Section: Introductionmentioning

confidence: 99%

“…Within the studies undertaken, researchers reported that As bioaccessibility varied between in vitro methods, in soils and household dust respectively Li et al, 2014;Oomen et al, 2002). Moreover, variable As bioaccessibility has been observed with differences in the compositions of the gastric and small intestinal phases, in vitro pH and other parameters (e.g., soil/ solution ratio, residence time) (Juhasz et al, 2009Smith et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Variability of arsenic bioaccessibility and metabolism in soils by human gut microbiota using different in vitro methods combined with SHIME

Yin

Zhang

et al. 2016

Science of The Total Environment

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• Five in vitro gastrointestinal methods combined with SHIME (colon phase) were used.• As bioaccessibility varied in the colon phase among these methods.• Plenty of As(III) and methylated arsenicals by microbial transformation were observed.• As bioaccessibility in PBET/SBRC-SHIME colon phase were closer to in vivo results for NIST 2710a. Arsenic (As) speciation analysis is essential when evaluating the risks upon oral exposure to As-contaminated soils. In this study, we first investigated the variability in the As bioaccessibility and speciation using a combination of five common in vitro methods (SBRC, PBET, DIN, UBM and IVG) (gastric and small intestinal phases) and the SHIME model (colon phase). Our results indicate that the As bioaccessibility varies in the colon phase. An increase in the As bioaccessibility for SBRC and PBET, and a decrease for UBM and IVG were observed in the colon phase. In addition, we found different extents of methylation and large amounts of arsenite [As(III)] due to microbial reduction in the colon digests. The UBM-SHIME method displayed a higher methylation percentage of 13.5-82.1%, but a lower methylation percentage of 0.2-21.8% was observed in the SBRC-SHIME method. Besides, The MMA V levels in the colon digests were positively correlated with those of As(III) and DMA V , so DMA V can be considered an indicator to evaluate the As metabolic speed of in vitro cultured human gut microbiota. Based on the standard reference soil of NIST 2710a, the As bioaccessibility in the colon phase of PBET-SHIME and SBRC-SHIME were the closest to the in vivo results. Combining in vitro methods and SHIME will remarkably affect the accurate assessment of potential risks to human health associated with oral exposure to soil As.

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Section: Arsenic Speciation Analysismentioning

confidence: 70%

Section: Arsenic Speciation Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Variability of arsenic bioaccessibility and metabolism in soils by human gut microbiota using different in vitro methods combined with SHIME

Yin

Zhang

et al. 2016

Science of The Total Environment

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In vitro bioaccessibility round robin testing for arsenic and lead in standard reference materials and soil samples

Dodd,

Lee,

Nelson

et al. 2024

Integr Envir Assess & Manag

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In this study, we assessed the suitability of using a standard reference material (SRM) other than National Institute of Standards and Technology (NIST) 2710a or NIST 2711a in USEPA Method 1340 to determine arsenic (As) and lead (Pb) in vitro bioaccessibility (IVBA) and the capabilities of Canadian‐based laboratories to perform the method. Five laboratories participated in an initial round robin study and analyzed NIST 2710a, NIST 2711a, BGS119, and Enviromat SS‐2. Intra‐ and inter‐laboratory variability were generally acceptable with percentage relative standard deviations (RSD) of less than 20%. The mean total As and Pb concentrations obtained for BGS119 (332 and 936 mg/kg, respectively) and the mean IVBA values (As = 14.3% and Pb = 78.1%) suggested it may be a suitable and acceptable SRM, whereas the concentration of As in Enviromat SS‐2 as received (3.2 mg/kg) was deemed too low. Ten soil samples from sites with varying land use were analyzed in a follow‐up round robin study using the modified IVBA method that included BGS119 as SRM. The concentrations of As and Pb in the IVBA extracts reported by the participating laboratories were comparable. The mean As IVBA values for the field‐collected samples ranged from 0.1% to 56.4%; for Pb, they ranged from 7.0% to 121%. The lowest IVBA values were measured in mine site samples; the highest values were associated with smelter‐affected soils. The low IVBA values correlated with high iron content. Intra‐ and interlaboratory reproducibility were acceptable (RSD < 30%). Based on the findings of the study, laboratories can use the modified method to provide reproducible and comparable As and Pb IVBA data. The use of BGS119 as an alternative SRM to assess contaminated sites in the province of British Columbia for regulatory purposes is recommended, as it is representative of As and Pb concentrations in contaminated soils in British Columbia. Integr Environ Assess Manag 2024;00:1–10. © 2024 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).

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Arsenic immobilization in soil affected by mining waste using waste‐derived functional hydrochar and iron‐encapsulated materials

Haris

Netherway²,

Eshtiaghi

et al. 2023

J of Env Quality

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Arsenic (As) contamination is a widespread problem. Continued and concerted effort in exploring sustainable remediation strategies is required, with in situ immobilization emerging as a promising option. This work valorized a waste by‐product from olive (Olea europaea L.) milling into functional hydrochar (HC). The HC was then transformed into iron oxide–encapsulated carbon with three different iron loading rates (10, 25, and 50% w/w of iron chloride hexahydrate added to the olive mill waste feedstock). The HC and the three iron oxide–encapsulated carbon materials were then tested in a pot trial using a 3% w/w application rate as a means to immobilize As in a mining‐contaminated soil (2,580 ± 110 mg kg−1 As). After a 45‐d incubation period, the effect of adding the amendments on As mobility and bioaccessibility compared with an untreated control was measured using a sequential extraction procedure and in vitro bioaccessibility, respectively. All four treatments resulted in a decrease in mobility and in vitro bioaccessibility as compared with the control. Specifically, As in the mobile phases was up to 35% less than the in control, whereas bioaccessibility was 21.8% in the control and ranged from 17.5 to 12.3% in the treatments. The efficiency of amendments to immobilize As increased with the iron content of the developed materials. This work positions HCs and iron oxide–encapsulated carbon materials produced from olive mill waste as promising options to immobilize As in situ.

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Assessment of Four Commonly Employed in Vitro Arsenic Bioaccessibility Assays for Predicting in Vivo Relative Arsenic Bioavailability in Contaminated Soils

Cited by 175 publications

References 28 publications

Variability of arsenic bioaccessibility and metabolism in soils by human gut microbiota using different in vitro methods combined with SHIME

Variability of arsenic bioaccessibility and metabolism in soils by human gut microbiota using different in vitro methods combined with SHIME

In vitro bioaccessibility round robin testing for arsenic and lead in standard reference materials and soil samples

Arsenic immobilization in soil affected by mining waste using waste‐derived functional hydrochar and iron‐encapsulated materials

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