Assessment of genetic and epigenetic variation in hop plants regenerated from sequential subcultures of organogenic calli

Peredo, Elena L.; Revilla, M. A.; Arroyo-García, Rosa

doi:10.1016/j.jplph.2005.09.010

Cited by 84 publications

(77 citation statements)

References 30 publications

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“…This mode of methylation dynamics is consistent with a gradual and perhaps stochastic process leading to denser methylation in advanced stages. In this context, methylation changes were more pronounced in plants regenerated from advanced stages of callus growth compared with those of earlier passages (Peredo et al, 2006).…”

Section: Possible Mechanism Of Callus-induced Methylation Changesmentioning

confidence: 88%

Cell Culture-Induced Gradual and Frequent Epigenetic Reprogramming of Invertedly Repeated Tobacco Transgene Epialleles

et al. 2009

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Using a two-component transgene system involving two epiallelic variants of the invertedly repeated transgenes in locus 1 (Lo1) and a homologous single-copy transgene locus 2 (Lo2), we have studied the stability of the methylation patterns and trans-silencing interactions in cell culture and regenerated tobacco (Nicotiana tabacum) plants. The posttranscriptionally silenced (PTGS) epiallele of the Lo1 trans-silences and trans-methylates the target Lo2 in a hybrid (Lo1/Lo2 line), while its transcriptionally silenced variant (Lo1E) does not. This pattern was stable over several generations in plants. However, in early Lo1E/Lo2 callus, decreased transgene expression and partial loss of Lo1E promoter methylation compared with leaf tissue in the parental plant were observed. Analysis of small RNA species and coding region methylation suggested that the transgenes were silenced by a PTGS mechanism. The Lo1/Lo2 line remained silenced, but the nonmethylated Lo1 promoter acquired partial methylation in later callus stages. These data indicate that a cell culture process has brought both epialleles to a similar epigenetic ground. Bisulfite sequencing of the 35S promoter within the Lo1 silencer revealed molecules with no, intermediate, and high levels of methylation, demonstrating, to our knowledge for the first time, cell-to-cell methylation diversity of callus. Regenerated plants showed high interindividual but low intraindividual epigenetic variability, indicating that the callusinduced epiallelic variants were transmitted to plants and became fixed. We propose that epigenetic changes associated with dedifferentiation might influence regulatory pathways mediated by trans-PTGS processes.

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Section: Possible Mechanism Of Callus-induced Methylation Changesmentioning

confidence: 88%

Cell Culture-Induced Gradual and Frequent Epigenetic Reprogramming of Invertedly Repeated Tobacco Transgene Epialleles

et al. 2009

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“…Bisulfite sequencing of the promoter in callus revealed molecules with no, intermediate, and high levels of methylation, demonstrating cell-to-cell methylation diversity in callus. Among regenerated plants the DNA methylation pattern was highly variable, but within a regenerated plant most cells were very similar in DNA methylation, indicating that the callus-induced epiallelic variants were transmitted to plants and had become fixed (Peredo et al 2006). Dedifferentiation in cell suspensions and calluses of Arabidopsis thaliana led to hypermethylation of promoters of various genes.…”

Section: Detection Of Epigenetic Statusmentioning

confidence: 99%

Epigenetics in plant tissue culture

Smulders¹,

Klerk²

2010

Plant Growth Regul

220

136

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Plants produced vegetatively in tissue culture may differ from the plants from which they have been derived. Two major classes of off-types occur: genetic ones and epigenetic ones. This review is about epigenetic aberrations. We discuss recent studies that have uncovered epigenetic modifications at the molecular level, viz., changes in DNA methylation and alterations of histone methylation or acetylation. Various studies have been carried out with animals, and with plant cells or tissues that have grown in tissue culture but only little work has been done with shoots generated by axillary branching. We present various molecular methods that are being used to measure epigenetic variation. In micropropagated plants mostly differences in DNA methylation have been examined. Epigenetic changes are thought to underlie various well-known tissue-culture phenomena including rejuvenation, habituation, and morphological changes such as flower abnormalities, bushiness, and tumorous outgrowths in, among others, oil palm, gerbera, Zantedeschia and rhododendron.

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“…However, changes at several levels, including morphological, biochemical, genetic and/or epigenetic, have been observed during in vitro plant propagation (Rahman and Rajora 2001;Etienne and Bertrand 2003;Peredo et al 2006;El-Dougdoug et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster)

et al. 2009

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Somatic embryogenesis (SE) is a propagation tool of particular interest for accelerating the deployment of new high-performance planting stock in multivarietal forestry. However, genetic conformity in in vitro propagated plants should be assessed as early as possible, especially in long-living trees such as conifers. The main objective of this work was to study such conformity based on genetic stability at simple sequence repeat (SSR) loci during somatic embryogenesis in maritime pine (Pinus pinaster Ait.). Embryogenic cell lines (ECLs) subjected to tissue proliferation during 6, 14 or 22 months, as well as emblings regenerated from several ECLs, were analyzed. Genetic variation at seven SSR loci was detected in ECLs under proliferation conditions for all time points, and in 5 out of 52 emblings recovered from somatic embryos. Three of these five emblings showed an abnormal phenotype consisting mainly of plagiotropism and loss of apical dominance. Despite the variation found in somatic embryogenesis-derived plant material, no correlation was established between genetic stability at the analyzed loci and abnormal embling phenotype, present in 64% of the emblings. The use of microsatellites in this work was efficient for monitoring mutation events during the somatic embryogenesis in P. pinaster. These molecular markers should be useful in the implementation of new breeding and deployment strategies for improved trees using SE.

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Assessment of genetic and epigenetic variation in hop plants regenerated from sequential subcultures of organogenic calli

Cited by 84 publications

References 30 publications

Cell Culture-Induced Gradual and Frequent Epigenetic Reprogramming of Invertedly Repeated Tobacco Transgene Epialleles

Cell Culture-Induced Gradual and Frequent Epigenetic Reprogramming of Invertedly Repeated Tobacco Transgene Epialleles

Epigenetics in plant tissue culture

Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster)

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