Assessment of genetic diversity among alfalfa (Medicago sativa L.) genotypes by morphometry, seed storage proteins and RAPD analysis

Živković, Bogovid; Radovic, J.; Sokolović, D.; Šiler, Branislav; Banjanac, Tijana; Štrbanović, Ratibor

doi:10.1016/j.indcrop.2012.03.027

Cited by 27 publications

(17 citation statements)

References 25 publications

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“…In response to the huge genetic diversity in alfalfa (Chandra and Pandey, 2011;Živković et al, 2012) and in respect to differences in environmental and ecological conditions, H. postica itself shows large geographic variation with different local adaptations. Accordingly, the alfalfa weevil with its wide distribution in the world (Holarctic region) (Skuhrovec, 2013) can be separated into several genetic lineages (Böttger et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Developmental differences of local populations of alfalfa weevil (Hypera postica) (Coleoptera: Curculionidae)

Sanaei¹,

Seiedy²

2016

Turk J Zool

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Many invasive insect pests show phenotypic variation between local populations across their geographical range. Understanding and integrating such differences will help to improve pest management. Population differences are the result of local adaptation to environmental conditions and may be detected using ecological analyses. Immature stages may represent especially interesting study objects as they represent important stages in the life cycle of insects and in many cases may be the most destructive stage. The alfalfa weevil, Hypera postica (Gyllenhal, 1813), is a variable worldwide pest that heavily damages alfalfa fields and is widely distributed in Iran. The lack of information about Iranian populations of H. postica has motivated us to examine immature life stages of three western populations (Karaj, Hamedan, Tuyserkan) and one eastern population (Jovein) under laboratory conditions. We measured the percentage of survival and the developmental times of each immature stage. The results suggested that the mean duration of the pupa stage significantly differs among the eastern population and all western populations (P < 0.005). Egg survivorship was lower in the eastern population (72.5%). The importance of differences in development times between populations is discussed in regards of pest management strategies.

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Section: Introductionmentioning

confidence: 99%

Developmental differences of local populations of alfalfa weevil (Hypera postica) (Coleoptera: Curculionidae)

Sanaei¹,

Seiedy²

2016

Turk J Zool

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“…Constantly maintenance genetic diversity and variability of alfalfa breeding pool during the selection through introductions of distances materials as a new source of genetic diversity is a prerequisite for successful breeding and developing of new cultivars/populations with high potential for yield and forage quality (Tucak et al, 2011;Živković et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Agro-Morphological and Forage Quality Traits of Selected Alfalfa Populations and Their Application in Breeding

Tucak¹,

Popoviã²,

Ãupiã³

et al. 2014

Turkish Journal of Field Crops

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“…The random amplified polymorphic DNA‐polymerase chain reaction (RAPD‐PCR) technique shows extensive divergence among, but little variation within, species. Therefore, it is extensively used to distinguish biotypes and/or germplasm accessions within weed species and crops (Volenberg et al ., ; Zivkovic et al ., ), as well as to differentiate races or isolates of plant pathogens (Gómez‐Lama Cabanás et al ., ; Villarino et al ., ). Genetic diversity based on RAPD markers has been also assessed among Orobanche spp.…”

Section: Introductionmentioning

confidence: 99%

Pathogenic and molecular diversity in highly virulent populations of the parasitic weedOrobanche cumana(sunflower broomrape) fromEurope

et al. 2013

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Summary The parasitic weed Orobanche cumana (sunflower broomrape) constrains sunflower production in eastern and southern Europe and in the Middle East. Although genetic resistance is the most effective control method, new parasite races evolve overcoming sunflower resistance. In this work, highly virulent populations of O. cumana were analysed for pathogenicity and genetic diversity. The virulence of 11 populations from Hungary, Romania, Spain and Turkey was assessed and compared after infection of sunflower inbred lines to differentiate races of the parasite under glasshouse conditions. Molecular diversity among and within 27 parasite populations was studied by RAPD‐PCR, UPGMA and amova analyses. Highly virulent race F was identified in Hungary, Spain and Turkey. The most virulent race (G) was also found in Turkey. The molecular analysis among highly virulent populations of O. cumana identified four molecular clusters, respectively, grouping populations from Central Spain, Hungary, South Spain and Turkey. The genetic homogeneity within parasite populations was confirmed, since no molecular divergences were found within them. This work constitutes the first geographical study of O. cumana together with pathogenicity and molecular traits inherent to each geographical group, and provides useful information for possible phylogenetic analyses of O. cumana. In addition, molecular markers associated with geographical origin could be developed and used as diagnostic tools to track new broomrape introductions into areas free of virulent races where they might represent a threat to sunflower production.

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Assessment of genetic diversity among alfalfa (Medicago sativa L.) genotypes by morphometry, seed storage proteins and RAPD analysis

Cited by 27 publications

References 25 publications

Developmental differences of local populations of alfalfa weevil (Hypera postica) (Coleoptera: Curculionidae)

Developmental differences of local populations of alfalfa weevil (Hypera postica) (Coleoptera: Curculionidae)

Agro-Morphological and Forage Quality Traits of Selected Alfalfa Populations and Their Application in Breeding

Pathogenic and molecular diversity in highly virulent populations of the parasitic weedOrobanche cumana(sunflower broomrape) fromEurope

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