Assessment of genetic diversity among soybean genotypes differing in response to aerial blight (Rhizoctonia solani Kuhn) using SSR markers

Abstract

Forty-seven genotypes and one wild relative of soybean, Glycine soja, were screened for resistance against aerial blight under epiphytotic conditions in the field during the Kharif season of two consecutive years viz., 2016 and 2017. Out of the 48 genotypes screened, only 18 genotypes exhibited a moderately resistant response to aerial blight during both the years of study. In order to perform molecular screening of the genotypes for aerial blight resistance, the genomic DNA obtained from the seedlings of the forty-eight soybean genotypes was subjected to PCR amplification with 12

Population Genetics and Anastomosis Group’s Geographical Distribution of Rhizoctonia solani Associated with Soybean

“…R. solani is a soil borne pathogen that affects soybean worldwide and has a significant economic impact in all soybean growing countries [ 1 , 7 , 11 , 19 , 21 , 59 , 60 , 61 ]. All AG associated with soybean reported in this study belonged to R. solani .…”

“…In Canada, root rot ranked fourth among 22 diseases causing severe losses in soybean [ 65 , 66 ]. In India, foliar blight caused by AG-1 caused an average yield loss of 40% to 50% [ 1 , 43 , 67 , 68 ]. Besides, AG-2 and AG-5 have been reported to cause hypocotyl rot of soybean in Japan [ 44 ].…”

“…Our study revealed that the rDNA-ITS sequences were clustered consistently according to their known AG and not according to geographical origin. Cluster analyses based on rDNA-ITS on sequences of R. solani AG and AG subgroups associated with a soybean of a specific geographical origin have already been reported [ 1 , 7 , 19 , 21 , 61 ].…”

“…Soybean ( Glycine max L.) is one of the world’s most significant oilseed crops, accounting for 25% of all edible oil production [ 1 ]. About 176.6 million tons of soybeans are produced over 75.5 million hectares of fertile land each year [ 2 , 3 ].…”

“…Information on the genetic diversity and distribution of R. solani AG associated with soybean of a particular country is available [ 1 , 42 , 43 , 44 ]. However, there was no attempt to investigate the global genetic diversity and distribution of R. solani AG associated with soybean.…”