Assessment of genetic diversity and relatedness among Tunisian almond germplasm using SSR markers

Gouta, Hassouna; Ksia, E.; Buhner, T.; Sánchez, María Ángeles Moreno; Zarrouk, Mokhtar; Mliki, Ahmed; Gogorcena, Yolanda

doi:10.1111/j.1601-5223.2009.02147.x

Cited by 30 publications

(16 citation statements)

References 39 publications

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“…For Tunisian almonds, a study by Gouta et al (2010) using ten almond SSR primers led to very similar conclusions to those drawn in an earlier RAPD analysis (Gouta et al, 2008). The mean observed heterozygosity was 0.68.…”

supporting

confidence: 67%

Review of genetic diversity studies in almond (Prunus dulcis)

Szikriszt

Hegedűs

Halász

2011

Acta Agronomica Hungarica

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Almond [Prunus dulcis (Mill.) D.A. Webb.] is cultivated for its nuts and is one of the oldest domesticated plants. Due to the genetically controlled self-incompatibility system that operates in this species, almond is characterized by great genetic diversity, the estimation of which has benefited from a range of marker techniques, including morphological characteristics, isoenzyme detection and molecular markers. Among the DNA-based molecular markers, simple sequence repeats (SSR) have been used most widely, although analyses have ranged from restriction fragment length polymorphism to the most recent single nucleotide polymorphism detection methods. Molecular markers have also been used to trace specific agronomic traits, e.g. self-(in)compatibility or kernel bitterness. Genetic diversity studies in almond have not revealed a direct relationship between the level of diversity and the origin of the germplasm. This might be explained by the relatively recent occurrence of self-compatibility in almond, which has not yet caused a serious loss of genetic diversity. The markers reviewed will be useful in monitoring and maintaining genetic diversity in almond breeding programmes, while others may permit marker-assisted selection for favourable agronomic traits. The cultivation, breeding and conservation of wild-growing almonds may equally benefit from the genetic diversity studies (especially those applying molecular markers).

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supporting

confidence: 67%

Review of genetic diversity studies in almond (Prunus dulcis)

Szikriszt

Hegedűs

Halász

2011

Acta Agronomica Hungarica

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“…Because there were only 13 cultivar denominations, clonal variation among cultivars or even homonyms may exist, i.e., same name for different cultivars. Homonyms are very common in traditional plant material and have been detected by microsatellites in several fruit species such as almond (Gouta et al, 2010), grape (Fernández-González et al, 2007;Boz et al, 2011), pear (Brini et al, 2008), olive (Isik et al, 2011), and chestnut (Gobbin et al, 2007). Different allelic profiles have been detected among samples of 'Maguillo', 'Pero Minguela', and 'Castellana' cultivars.…”

Section: Discussionmentioning

confidence: 99%

Molecular and morphological characterization of local apple cultivars in Southern Spain

Pérez-Romero¹,

Suárez²,

Fuente³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The number of local and traditional fruit cultivars in Andalusia (Southern Spain) has decreased dramatically since the 1970s when new commercial cultivars from breeding programs were introduced, replacing old varieties, and thus decreasing genetic diversity. The present study was included in a genetic resources project with the objective of identifying and preserving traditional fruit tree cultivars in Southern Spain. The goal of this study was to begin the characterization of 29 apple accessions (Malus x domestica Borkh) belonging to 13 traditional cultivar denominations. For molecular characterization studies, 12 simple sequence repeat markers previously developed for apple species were used. Morphological characterization was performed using 33 fruit traits. A total of 115 alleles were amplified for the 12 loci, ranging from 7 (CH01h01, CH01h10, and GD 12) to 13 alleles per locus (CH02c11). Forty-one alleles were unique to specific genotypes. The locus with the highest number of detected unique alleles was CH01f03b with 6 alleles. Expected heterozygosity

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“…The clear distinction between the northern from one side and the central and southern populations from the other side is definitely due to the natural selection. These results, reveal the high diversity and the distinct origin of the Tunisian almond germplasm and can be considered as another statement in favor of the hypothesis advanced by Gouta et al (2010Gouta et al ( , 2012) regarding a distinct parental and origin of our local cultivars. Thus, prospecting new sites and helping farmers to preserve on farm large diversity will guaranty a sustainable and valueble source of traits for future breeding goals at an international level especially with the actual threats of global warming and its negative effects on biodiversity.…”

Section: Locusmentioning

confidence: 56%

“…Amplification reactions were carried out in a final volume of 15 l containing 10 ng of template DNA and PCR reagents as described in Gouta et al (2010) with a Gene Amp 2700™ thermocycler (Applied Biosystems, CA, USA) using the following temperature cycles: 1 cycle of 3 min at 95°C; 35 cycles of 1 min at 94°C, 45 s at the corresponding annealing temperature (Table 2) and 1 min at 72°C. The last cycle was followed by a final incubation for 7 min at 72°C and the PCR products were stored at 4°C before analysis.…”

Section: Dna Amplificationmentioning

confidence: 99%

“…These markers have been used for the molecular characterization and estimation of genetic diversity among different Prunus species, including peach Bouhadida et al, 2007Bouhadida et al, , 2011Dirlewanger et al, 2002 andMartínez-Gómez et al, 2003a), sweet cherry (Wunsch and Hormaza, 2002) and apricot cultivars (Hormaza, 2002;Maghuly et al, 2005). Moreover, SSRs are currently being employed for molecular characterization, estimation of genetic diversity and genetic relationships among almond cultivars (Gouta et al, 2010) and other related species (Martínez-Gómez et al, 2003b;Sánchez-Pérez et al, 2006;Shiran et al, 2007;Xu et al, 2004;Zeinalabedini et al, 2008), but to our knowledge, no population identification of the Tunisian almond germplasm was carried out and molecular variance (AMOVA) analysis is still lacking.…”

Section: Introductionmentioning

confidence: 99%

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Molecular variance of the Tunisian almond germplasm assessed by simple sequence repeat (SSR) markers

Gouta¹,

Ksia²,

Ahmed³

et al. 2013

Afr. J. Biotechnol.

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The genetic variance analysis of 82 almond (Prunus dulcis Mill.) genotypes was performed using ten genomic simple sequence repeats (SSRs). A total of 50 genotypes from Tunisia including local landraces identified while prospecting the different sites of Bizerte and Sidi Bouzid (Northern and central parts) which are the most important locations of almond diversity in Tunisia were included. Analysis of molecular variance (AMOVA) was performed for 11 populations from these different regions and foreign countries to examine the distribution of genetic variation of the accessions studied. Results show that the major variation occurred within populations in each geographic site. Additionally, this analysis demonstrates that the genetic diversity within local almond cultivars was important, with a clear geographic distinction between the Northern and the Southern Tunisian cultivars. The value of prospecting new sites, preserving genetic diversity and encouraging on farmers almond collections is also discussed.

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Assessment of genetic diversity and relatedness among Tunisian almond germplasm using SSR markers

Cited by 30 publications

References 39 publications

Review of genetic diversity studies in almond (Prunus dulcis)

Review of genetic diversity studies in almond (Prunus dulcis)

Molecular and morphological characterization of local apple cultivars in Southern Spain

Molecular variance of the Tunisian almond germplasm assessed by simple sequence repeat (SSR) markers

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