Assessment of Genetic Diversity and Population Structure of Tunisian Barley Accessions (Hordeum vulgare L.) Using SSR Markers

Marzougui, Salem; Kharrat, Mohamed; Younes, M. Ben

doi:10.5586/aa.7343

Cited by 6 publications

(5 citation statements)

References 23 publications

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“…The 32 barley used in this study were evaluated for morphological traits, grain yield, yield components and physiological traits. These traits are targeted to increase the genetic variability and thus selection in barely breeding programs [31]. In addition, assessing genetic diversities is important in identifying genotypes with good phenotypic traits [32] and distinct genetic groups [33].…”

Section: Discussionmentioning

confidence: 99%

Characterization Barley Genotypes Adaptability to Semi-Arid Environment

Ghazy,

Motawei,

Ali

et al. 2024

Preprint

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Barley is the fourth largest grain crop globally and used for food and feed.It is necessary to evaluate those germplasm responses and stabilities under agricultural practices of respected geographical regions before. A set of 32 new barley genotypes were evaluated under desert environments to identify superior genotypes based on their agro-morphological characters and molecular markers diversity. In general, the developed Saudi barley lines were superior to the landraces and checks cultivars for several traits. The KSU-BR-G121/L-4 line had the highest grain yield and harvest index in the second season, while the inbred line KSU-BR-C/G-2 had the highest grain yield and harvest index in the first season. The line KSU-BR-40-18-4 had the highest grain protein content 15.24 and 15.12 (six row). The two-row ICARDA line (Carina/Moroc9-75) had the highest grain and flour protein, 17.63 and 13.74 respectively. The barley inbred lines revealed a considerable diversity level based on agro-phenotypic and molecular characterization indicating the possibility to improve for semi-arid conditions. The introduction of novel Saudi barley lines and more comprehensive use of genetic resources could be important tool to increase the genetic variability within Saudi barley breeding programs.

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Section: Discussionmentioning

confidence: 99%

Characterization Barley Genotypes Adaptability to Semi-Arid Environment

Ghazy,

Motawei,

Ali

et al. 2024

Preprint

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“…Moreover, systematic relationships between barley families were examined using ISSR, SRAP, and ISTR data to determine molecular phylogeny, with ISTR and SRAP markers showing good discriminating power for defining genetic variation [ 36 ]. Similarly, TRAP markers distinguished agricultural types effectively, while the SRAP marker dendrogram classified Egyptian barley cultivars into distinct groups based on genetic similarity coefficients [ 53 – 57 ].…”

Section: Discussionmentioning

confidence: 99%

“…The clusters consisted of distinct cultivars that were found to be unevenly distributed. Cluster 1 was mainly composed of cultivars like Rihane and Lemsi, while Cluster 2 featured cultivars like Kounouz and Manel [ 53 ]. Similarly, analysis by Mohammadi et al indicated a peak delta K value at K = 3, supporting categorizing populations into three major subpopulations corresponding to Iranian landraces, foreign landraces, and varieties and advanced breeding lines [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular and agro-morphological characterization of new barley genotypes in arid environments

Elshafei,

Ibrahim,

Abdellatif

et al. 2024

BMC Biotechnol

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Background Genetic diversity, population structure, agro-morphological traits, and molecular characteristics, are crucial for either preserving genetic resources or developing new cultivars. Due to climate change, water availability for agricultural use is progressively diminishing. This study used 100 molecular markers (25 TRAP, 22 SRAP, 23 ISTR, and 30 SSR). Additionally, 15 morphological characteristics were utilized to evaluate the optimal agronomic traits of 12 different barley genotypes under arid conditions. Results Substantial variations, ranging from significant to highly significant, were observed in the 15 agromorphological parameters evaluated among the 12 genotypes. The KSU-B101 barley genotype demonstrated superior performance in five specific traits: spike number per plant, 100-grain weight, spike number per square meter, harvest index, and grain yield. These results indicate its potential for achieving high yields in arid regions. The Sahrawy barley genotype exhibited the highest values across five parameters, namely leaf area, spike weight per plant, spike length, spike weight per square meter, and biological yield, making it a promising candidate for animal feed. The KSU-B105 genotype exhibited early maturity and a high grain count per spike, which reflects its early maturity and ability to produce a high number of grains per spike. This suggests its suitability for both animal feed and human food in arid areas. Based on marker data, the molecular study found that the similarity coefficients between the barley genotypes ranged from 0.48 to 0.80, with an average of 0.64. The dendrogram constructed from these data revealed three distinct clusters with a similarity coefficient of 0.80. Notably, the correlation between the dendrogram and its similarity matrix was high (0.903), indicating its accuracy in depicting the genetic relationships. The combined analysis revealed a moderate correlation between the morphological and molecular analysis, suggesting alignment between the two characterization methods. Conclusions The morphological and molecular analyses of the 12 barley genotypes in this study effectively revealed the varied genetic characteristics of their agro-performance in arid conditions. KSU-B101, Sahrawy, and KSU-B105 have emerged as promising candidates for different agricultural applications in arid regions. Further research on these genotypes could reveal their full potential for breeding programs.

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“…This suggested that the higher gene flow across geographic regions and the variation among accessions was more important than looking for differences in geographic regions as a source of genetic distance among accessions to use in barley breeding programs. Other researchers also studied the population structure of barley genotypes and reported that there was more genetic variation among individuals within a population than among populations [ 13 , 37 , 63 ].…”

Section: Discussionmentioning

confidence: 99%

Analysis of genetic diversity and population structure of some Ethiopian barley (Hordeum vulgare L.) accessions using SSR markers

Zewodu,

Mohammed,

Shiferaw

2024

PLoS ONE

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Understanding the genetic diversity of existing genetic resources at the DNA level is an effective approach for germplasm conservation and utilization in breeding programs. However, the patterns of genetic diversity and population structure remain poorly characterized, making germplasm conservation and breeding efforts difficult to succeed. Thus, this study is aimed to evaluate the genetic diversity and population structure of 49 barley accessions collected from different geographic origins in Ethiopia. Twelve SSR markers were used to analyze all accessions and a total of 61 alleles were found, with a mean of 5.08 alleles per locus. The analysis pointed out the existence of moderate to high values of polymorphic information content ranging from 0.39 to 0.91 and the mean Shannon diversity index(I) was 1.25, indicating that they were highly informative markers. The highest Euclidean distance (1.32) was computed between accession 9950 and two accessions (247011 and 9949), while the lowest Euclidean distance (0.00) was estimated between accessions 243191 and 243192. The result of molecular variance analysis revealed that the highest variation was found among accessions (47) relative to within accessions (44) and among geographic origins (9). Cluster analysis grouped the 49 barley accessions into three major clusters regardless of their geographic origin which could be due to the presence of considerable gene flow (2.72). The result of the STRUCTURE analysis was consistent with neighbor-joining clustering and principal coordinate analysis. Generally, this study concluded that the variation among accessions was more important than the difference in geographical regions to develop an appropriate conservation strategy and for parental selection to use in breeding programs. This information will be helpful for barley conservation and breeding, and it may speed up the development of new competing barley varieties.

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Assessment of Genetic Diversity and Population Structure of Tunisian Barley Accessions (Hordeum vulgare L.) Using SSR Markers

Cited by 6 publications

References 23 publications

Characterization Barley Genotypes Adaptability to Semi-Arid Environment

Characterization Barley Genotypes Adaptability to Semi-Arid Environment

Molecular and agro-morphological characterization of new barley genotypes in arid environments

Analysis of genetic diversity and population structure of some Ethiopian barley (Hordeum vulgare L.) accessions using SSR markers

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