Assessment of genetic diversity and phylogenetic relationships of Korean native chicken breeds using microsatellite markers

Seo, Joo Hee; Lee, Jun Heon; Kong, Hong Sik

doi:10.5713/ajas.16.0514

Cited by 16 publications

(12 citation statements)

References 13 publications

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“…Several studies, using DNA fingerprinting, polymorphic microsatellite markers, and 600k SNP chip data, found evidence of such genetic introgressions into the KNC since 1960. Based on the previous results, KNC lines are closely related to Cornish, New Hampshire, and RIR breeds and genetically more distant from the white Leghorn breed ( Lee et al., 1996a , b ; Kong et al., 2006 ; Oh et al., 2008 ; Suh et al., 2014 ; Seo et al., 2017 ; Seo et al., 2018 ). Seo et al.…”

Section: Resultsmentioning

confidence: 91%

“…As the demand for native chicken meat has increased owing to its unique taste and meat quality, the original KNC breeds and commercial breeds have been widely used for diverse crossbreeding since 2000 ( Park et al., 2010 , 2011 ; Kang et al., 2010 ; Lee et al., 2013 , 2014 ). As a result, one of the famous hybrids called “Woorimatdag” produced by the NIAS and “Hanhyup-3-ho” of Hanhyup private company are available on the current market ( Choo and Chung, 2014 ; Seo et al., 2017 ).…”

Section: Introductionmentioning

confidence: 99%

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Major histocompatibility complex B variability in Korean native chicken breeds

et al. 2020

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Adaptive genetic variations have direct influences on the fitness traits of the animal. The major histocompatibility complex B ( MHC-B ) region is responsible for adaptive and innate immune responses in chickens. In native Korean chicken breeds, no information on serologically defined B haplotypes is available. We investigated the MHC-B diversity in 5 restored lines of Korean native chicken and Ogye chicken breeds using a recently described MHC-B single-nucleotide polymorphism ( SNP ) panel and the MHC-linked LEI0258 variable number of tandem repeat marker. High SNP haplotype diversity was observed in Korean native chicken breeds with an average of 9.7 MHC-B SNP haplotypes per line. The total number of haplotypes ranged from 6 to 12 per line, and population-specific haplotypes ranged from 3 to 4. A total of 41 BSNP haplotypes, including 26 novel population-specific haplotypes and 15 common haplotypes, were reported over all populations. The 15 common haplotypes included 7 novel and 8 previously reported standard haplotypes. Selection and breeding evidence supports the observation of common haplotypes between the Korean native chicken and exotic breeds. Similarly, the LEI0258 marker showed allele variation, between 193 bp and 474 bp having 5 to 8 alleles per population. Some of these alleles (193, 249, 309, and 443 bp) were shared and more frequently observed. Comparison between SNP haplotypes and LEI0258 allele sizes for the same samples showed that some LEI0258 allele sizes correspond to more than one BSNP haplotype. The use of the MHC-B SNP panel greatly enhances the identification of MHC diversity compared with the sole use of the LEI0258 marker in native chicken populations.

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Section: Resultsmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Major histocompatibility complex B variability in Korean native chicken breeds

et al. 2020

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“…The primers were LEI0258-F: 5′-CAC GCA GCA GAA CTT GGT AAGG-3′ and LEI0258-R: 5′-AGC TGT GCTC AGT CCT CAG TGC -3′ [30,31]. BF2 sequence-based typing (SBT) was performed by sequencing each product obtained from the exon 2 region of the chicken BF2 gene using the forward primer 5′-GCA GAG CTC CAT ACC CTG CGGTA-3′ and the reverse primer 5′-GCC GGT CTG GTT GTA GCG CCG-3′ [32,33]. All primers were synthesized by Bioneer Inc. PCR products were electrophoresed on a 1.5% agarose gel with 0.5 X TBE buffer.…”

Section: Genotypingmentioning

confidence: 99%

Characterization of splenic MRC1hiMHCIIlo and MRC1loMHCIIhi cells from the monocyte/macrophage lineage of White Leghorn chickens

Pyung

et al. 2020

Vet Res

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Monocytes/macrophages, which are found in a variety of organs, maintain tissue homeostasis at a steady state and act as the first line of defence during pathogen-induced inflammation in the host. Most monocyte/macrophage lineage studies in chickens have been largely performed using cell lines, while few studies using primary cells have been conducted. In the present study, the phenotypic and functional characteristics of splenic monocyte/macrophage lineage cells during steady state and inflammatory conditions were examined. Splenic monocyte/macrophage lineage cells could be identified as MRC1 lo MHCII hi and MRC1 hi MHCII lo cells based on their surface expression of MRC1 and MHCII. In the steady state, MRC1 lo MHCII hi cells were more frequently found among MRC1 + cells. MRC1 lo MHCII hi cells expressed a higher number of antigen-presenting molecules (MHCII, MHCI, and CD80) than MRC1 hi MHCII lo cells. In contrast, MRC1 hi MHCII lo cells showed better phagocytic and CCR5-dependent migratory properties than MRC1 lo MHCII hi cells. Furthermore, MRC1 hi MHCII lo cells infiltrated the spleen in vivo and then became MRC1 lo MHCII hi cells. During lipopolysaccharide (LPS)-induced inflammatory conditions that were produced via intraperitoneal (i.p.) injection, the proportion and absolute number of MRC1 hi MHCII lo cells were increased in the spleen. Uniquely, inflammation induced the downregulation of MHCII expression in MRC1 hi MHCII lo cells. The major source of inflammatory cytokines (IL-1β, IL-6, and IL-12) was MRC1 lo MHCII hi cells. Furthermore, MRC1 hi MHCII lo cells showed greater bactericidal activity than MRC1 lo MHCII hi cells during LPS-induced inflammation. Collectively, these results suggest that two subsets of monocyte/macrophage lineage cells exist in the chicken spleen that have functional differences.

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“…Microsatellite population studies are more frequently applied on local breeds e.g. in China [7], Oman [8], Korea [9] to clarify origin or position of these breeds relative to other ones. Population study can also be performed within a breed [10] to clarify herd position and highlight those populations which require attention by the management.…”

Section: Introductionmentioning

confidence: 99%