Assessment of Genetic Diversity Based on Issr Markers in Neopestalotiopsis Species Collected From Guava (Psidium Guajava L.) Plants Affected With Canker Disease in Pakistan

Haq, Imran Ul

doi:10.15666/aeer/1705_1180311811

Cited by 3 publications

(2 citation statements)

References 6 publications

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“…Several markers have been documented to scrutinize quinoa genotypes’ genetic variability and phylogenetic associations ( Fuentes et al, 2009 ; Zhang et al, 2017 ; Abd El-Moneim et al, 2021 ; El-Harty et al, 2021 ). Inter simple sequence repeat (ISSR) markers are powerful tools in genetic diversity analysis, as these are considered arbitrarily amplified dominant markers, cost-effective, and actively detect polymorphisms ( Thakur et al, 2018 ; Haq and Ijaz, 2019 ). These markers require a minute quantity of DNA and do not necessitate prior knowledge of DNA sequence.…”

Section: Introductionmentioning

confidence: 99%

Empirical phenotyping and genome-wide association study reveal the association of panicle architecture with yield in Chenopodium quinoa

Habib,

Ijaz,

Haq

et al. 2024

Front. Microbiol.

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Chenopodium quinoa manifests adaptability to grow under varying agro-climatic scenarios. Assessing quinoa germplasm’s phenotypic and genetic variability is a prerequisite for introducing it as a potential candidate in cropping systems. Adaptability is the basic outcome of ecological genomics of crop plants. Adaptive variation predicted with a genome-wide association study provides a valuable basis for marker-assisted breeding. Hence, a panel of 72 quinoa plants was phenotyped for agro morphological attributes and association-mapping for distinct imperative agronomic traits. Inter simple sequence repeat (ISSR) markers were employed to assess genetic relatedness and population structure. Heatmap analysis showed three genotypes were early maturing, and six genotypes were attributed for highest yield. The SD-121-07 exhibited highest yield per plant possessing green, glomerulate shaped, compact density panicle with less leaves. However, SJrecm-03 yielded less exhibiting pink, intermediate shape, intermediate density panicles with less leaves. The phenotyping revealed strong correlation of panicle architecture with yield in quinoa. A genome-wide association study unraveled the associations between ISSR makers and agro-morphological traits. Mixed linear modes analysis yielded nine markers associated with eight traits at p ≤ 0.01. Moreover, ISSR markers significantly associated with panicle shape and leafiness were also associated with yield per plant. These findings contribute to the provision of authenticity for marker-assisted selection that ultimately would support quinoa breeding programs.

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Section: Introductionmentioning

confidence: 99%

Empirical phenotyping and genome-wide association study reveal the association of panicle architecture with yield in Chenopodium quinoa

Habib,

Ijaz,

Haq

et al. 2024

Front. Microbiol.

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“…Primers for intermicrosatellite analysis, i.e., ISSR primers, contain a fragment of a microsatellite locus (most often di-or trinucleotide) and one or two "anchor" nucleotides on one of the repetition flanks. This method started to be developed in 1994 and has now become widely spread, especially in studying the gene pools of various plant species for mapping genomes and marking the agronomically important traits (Nasim et al, 2020;Ul Haq and Ijaz, 2019).…”

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confidence: 99%

Genetic State of Pulsatilla Patens (L.) Mill. Coenopopulations Based on DNA Labeling

Beishov¹,

Sultangazina²,

Бейшова³

et al. 2021

OnLine Journal of Biological Sciences

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In the article, the results of studying the gene pools of the Kazakhstan coenopopulations of a rare plant species Pulsatilla patens (L.) Mill. are shown. The biomaterial taken from five coenopopulations located in Northern Kazakhstan has been used as samples. To identify the multilocus DNA polymorphism, the authors have used ISSR (Inter Simple Sequence Repeats, or intermicrosatellite analysis) labeling. The most effective primers have been selected, which provide reproducible results with a length of 17-19 nucleotides. As a result, multiple genomic labeling of five Pulsatilla patens coenopopulations has been performed. The main indicators of genetic diversity (P95 -0.965, HE -0.162, I -0.373 for the total sample), the structure and differentiation of the populations (HT -0.232, HS -0.162, GST -0.3 for the total sample) and the type and state of the gene pools of the studied coenopopulations have been determined. The specificity of the gene pools has been characterized by the number of rare alleles and the Coefficient of Genetic Originality (CGO). The maximum value of CGO has been noted at Pp5 (2.964) and the minimum one -at Pp4 (2.666). The gene pools of the Pp1 and Pp4 coenopopulations have been characterized as typical and gene pools of the Pp2, Pp3 and Pp5 -as specific. Based on the obtained data, tree diagrams have been made that reflect the degree of similarity of the studied coenopopulations as to the ISSR spectra. Identification of the state of the gene pools of rare plant species will allow recommending scientifically based measures for preserving them.

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Selection and validation of reference genes for normalizing qRT‒PCR gene expression studies in Colletotrichum gloeosporioides and interaction with the guava plants

Haq,

Ijaz,

Hashem

et al. 2023

Front. Plant Sci.

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Quantitative real-time PCR is used to quantify gene expression, even to detect low-level transcripts. It detects and quantifies the inoculum level of fungal pathogens in infected hosts. However, reliable expression profiling data require accurate transcript normalization against a stable reference gene. Hence, using stably expressed reference genes under variable conditions is paramount in gene expression analysis. In the current study, reference genes were selected and validated in Colletotrichum gloeosporioides, a guava canker and dieback pathogen. The reference gene selection and validation in C. gloeosporioides were evaluated for germinated conidia and mycelium (in vitro) and in infected guava (Psidium guajava) (interaction with host plant). The CgCAL gene was determined as a highly stable reference gene, followed by the CgTUB2 in C. gloeosporioides for germinating conidia and mycelium. However, the CgTUB2 gene was determined to be a highly stable reference gene, followed by the CgCAL for expression analysis during its interaction with the plant. Expression profiling revealed stable and constant relative expression patterns of selected reference genes for both PR genes by determining their relative transcript level. This study is the first to describe reference gene selection and validation to quantify target gene expression in C. gloeosporioides.

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Assessment of Genetic Diversity Based on Issr Markers in Neopestalotiopsis Species Collected From Guava (Psidium Guajava L.) Plants Affected With Canker Disease in Pakistan

Cited by 3 publications

References 6 publications

Empirical phenotyping and genome-wide association study reveal the association of panicle architecture with yield in Chenopodium quinoa

Empirical phenotyping and genome-wide association study reveal the association of panicle architecture with yield in Chenopodium quinoa

Genetic State of Pulsatilla Patens (L.) Mill. Coenopopulations Based on DNA Labeling

Selection and validation of reference genes for normalizing qRT‒PCR gene expression studies in Colletotrichum gloeosporioides and interaction with the guava plants

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