Assessment of genetic diversity by simple sequence repeat markers among forty elite varieties in the germplasm for malting barley breeding

Abstract

Abstract:The genetic diversity and relationship among 40 elite barley varieties were analyzed based on simple sequence repeat (SSR) genotyping data. The amplified fragments from SSR primers were highly polymorphic in the barley accessions investigated. A total of 85 alleles were detected at 35 SSR loci, and allelic variations existed at 29 SSR loci. The allele number per locus ranged from 1 to 5 with an average of 2.4 alleles per locus detected from the 40 barley accessions. A cluster analysis based on the genetic similarity coefficients was conducted and the 40 varieties were classified into two groups. Seven malting barley varieties from China fell into the same subgroup. It was found that the genetic diversity within the Chinese malting barley varieties was narrower than that in other barley germplasm sources, suggesting the importance and feasibility of introducing elite genotypes from different origins for malting barley breeding in China.

Comparative Analysis of Agro-Morphological and Molecular Variations in Huskless Barley (Hordeum vulgare L.) under Central Agro-Climatic Zone of India

“…subgroup A with 14 accessions and subgroup B with 6 accessions. Similar results of clustering of genotypes of common ancestor in single group were observed by Wang et al, (2010). Zakova et al, (2006) got similar results for spring barley accessions.…”

Fungal diversity in adult date palm (Phoenix dactylifera L.) revealed by culture-dependent and culture-independent approaches

“…The literature presents various methods for the characterization of fungal flora. These include the determination of the growth parameters (Steinberg et al, 1997a), analysis of the diversity of β-tubulin sequences (Watanabe et al, 2011), ribosomal genes sequencing (Kodsueb et al, 2006;Crous et al, 2007;Schroers et al, 2009), intergenic 18S-28S internal transcribed spacer (ITS) sequencing (Camara et al, 2002;Braun et al, 2003;Pryor and Bigelow, 2003;Kwasna et al, 2006;Manamgoda et al, 2012), multilocus analysis (Zhang et al, 2009a), and single sequence repeat (SSR) genotyping (Wang et al, 2010). Further methods include terminal restriction fragment length polymorphism (T-RFLP) (Dickie and FitzJohn, 2007), amplified ribosomal DNA restriction analysis (ARDRA) (Sutthisa et al, 2010), as well as ribosomal intergenic spacer analysis (RISA) (Sigler and Zeyer, 2002).…”

Assessment of genetic diversity among released and elite Ethiopian barley genotypes using simple sequence repeat (SSR) markers

“…Accordingly, thirty five SSR markers were f ound f rom Wang et al (2010). All of them were screened for amplification and usefulness and 22 of them were found to be polymorphic ( Table 2).…”

“…Polymerase chain reaction was optimized starting from the reaction set up described in Wang et al (2010). Accordingly, PCR was carried out in a 25-μL final volume containing 2 μL of 20 ng/μL genomic DNA templates, 2.5 μL of 1X PCR buffer containing 15 mM Mg 2+ , 0.5 μL of 15 mM dNTP mixture (2.5 mM of each), 1.25 μL of 5 u/μL of Taq DNA polymerase, and 0.25 μL of 10 μM forward and reverse primers and 1.6 ng/μL of gDNA (20 ng/μL of stock) for amplification.…”

“…Ramsay et al (2000) developed SSR markers for molecular characterization and linkage mapping of barley. Molecular diversity of H. vulgare L. was studied using SSR markers (Wang et al, 2010;Hadado et al, 2010) and the primers were designed by Ramsay et al (2000). Chaabane et al (2009) also characterized barley collections of Tunisia, Syria and Denmark by SSR markers.…”